vsv2

Culture and identification of infectious agents Dr. Alvin Fox

Key Terms ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Taxonomy ,[object Object],[object Object],[object Object]

Species versus strains - selecting discriminating features

Classification ,[object Object],[object Object],[object Object],[object Object],[object Object]

Identification of infectious agents in the diagnostic laboratory ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Steps in isolation and identification ,[object Object],[object Object],[object Object],[object Object]

CDC/Dr. James Feeley Sheep blood agar plate culture Bacillus anthracis Bacillus cereus .

Isolation and identification ,[object Object],[object Object]

Gram negative Gram positive Heat/Dry Crystal violet stain Iodine Fix Safranin stain Alcohol de - stain

Gram stain morphology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Step 3: Isolated bacteria are speciated ,[object Object]

Clinical Microbiology Laboratory Bench

Step 4: Antibiotic susceptibility testing

Antibiotic susceptibility testing No growth Susceptible Not susceptible Bacterial lawn Growth Antibiotic disk

Molecular differentiation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

16S rRNA Sequencing ,[object Object],[object Object]

Real-time PCR Cycle one Cycle two Cycle 30 2 30 ds DNA Dye

DNA-DNA hybridization 100% Homology + Heat 0% Homology Strain 1 Strain 2

Profiles ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Protein profiling ,[object Object],[object Object]

Rapid diagnosis without culture ,[object Object],[object Object],[object Object]

Streptococcal Agglutination Test Latex beads Streptococcal antigenic extract Antibody

Bacterial DNA sequences amplified directly from human body fluids ,[object Object],[object Object],[object Object]

Microscopy ,[object Object],[object Object],[object Object]

Serologic identification ,[object Object],[object Object]

vsv2

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