This chapter discusses sterile processing standards and practices in different healthcare facilities. It provides an overview of sterile processing in ambulatory surgical centers, including their history, regulatory standards, and personnel. Standards and practices for dental facilities, including environmental and instrument processing procedures, are also examined. The chapter describes sterile processing in Veterans Affairs facilities and differences from other facilities. Finally, it emphasizes that the core science of cleaning, sterilization and handling sterile items is consistent, regardless of facility type.