Kts Gm Foods Around Us

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Tecnologia Negócios

GM Foods Around Us Kowloon Technical School Team Members Chan Kam Hung Chan Perth Chung Ka Leung Wong Tsun Man Yu Koon Ho CityU Project-based Outreach Programme 2009 19 September 2009

What are GM foods? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

What are the advantages of GM-crops? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

What are the disadvantages of GM-crops? ,[object Object],[object Object],[object Object],[object Object]

What is the objective of our project? ,[object Object]

What are the food samples? Soybeans bought from City’super in Harbour City. (Origin: Canada) Soybeans bought from a market in Sham Shui Po. (Origin: USA) Sample 2 Sample 1

Pakfook tofu Sample 4 San Sui tofu Vitasoy Sample 5 Sample 3

Methodology ,[object Object],[object Object],[object Object],[object Object],[object Object]

1. Protein-based Method - ELISA ,[object Object],[object Object],Eurofins TraitChek RoundupReady TM Bulk Soybean Grain was used for ELISA test.

1. Protein-based Method - ELISA ,[object Object],[object Object],[object Object],[object Object]

Principles of ELISA ,[object Object],[object Object],[object Object]

ELISA Result -ve 5. San Sui tofu -ve 4. Pakfook tofu -ve 3. Vitasoy -ve 2. Soybean (Canada) -ve 1. Soybean (USA) Results Sample

Discussion of ELISA ,[object Object],[object Object],[object Object],[object Object]

2. DNA-based Method ,[object Object],[object Object],[object Object],[object Object],[object Object]

2. DNA-based Method - PCR ,[object Object],[object Object],[object Object]

Detection of soybean lectin (192 bp) ,[object Object],[object Object],GCC CAT CTG CAA GCC TTT TTG GM04 GAC CTC CTC GGG AAA GTT AC lectin 1a Sequence Primer

Detection of Roundup Ready resistance gene (RR) (356 bp) ,[object Object],[object Object],CCC CAA GTT CCT AAA TCT TCA AGT RR04 TGG CGC CCA AAG CTT GCA TGG C RR01 Sequence Primer

2. DNA-based Method – DNA Electrophoresis

Result of DNA Electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Conclusion ,[object Object],[object Object],[object Object],[object Object]

References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Acknowledgement ,[object Object],[object Object],[object Object],[object Object]

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