Background: Business Process Outsourcing Sector (BPO) sector is one of the fastest growing industrial segments in India. However, the industry is plagued by high attrition rates. Different surveys do indicate musculoskeletal disorders (MSD) and stress related problems by prolonged night shifts and stressful working conditions. To address these issues, there is paucity of information regarding ergonomic design of work place, working conditions, stress and MSD for Indian night shift employees of BPO sector. Secondary data relating the psycho physiological aspects relating to the work is examined to build context for the present study. Research address the issues of impact of violation of circadian rhythm, stressful and anxious work environment, availability of ergonomic work environment design and low job satisfaction of night shift BPO employees. Methods: Survey data collected in 2011 among the workforce of ten organizations in Chennai is used for this study. The sample study covered 106 employees. Results: The output of research emphasis need for multi disciplinary approach consisting of ergonomics, occupational therapy and psychology to improve physical and psychological well being of employees to address issues of attrition in BPO industry.