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The Molecular Basis of
                 Photosynthesis

         Katy Jordan, University of Cambridge
                   klj33@cam.ac.uk



ALT-C
8th September 2010               Background credit:
Nottingham, UK                   Matthew Fang at Flickr
Why is it important to understand
                   photosynthesis?

  • Photosynthesis is fundamental to our past and our
  future
  • The most important biochemical process on Earth!
  • Holds key to sustainable energy?




ALT-C
8th September 2010
Nottingham, UK
How is the concept of photosynthesis taught?

       The process by which carbon
       dioxide is converted to sugar

       6CO2 + 6H20  C6H12O6 + 6O2


ALT-C
8th September 2010
Nottingham, UK
How is the concept of photosynthesis taught?




ALT-C
                     http://www.flickr.com/photos/epsos/3384297473/sizes/m/in/photostream/
8th September 2010
Nottingham, UK                               http://school.edturtle.co.uk/photosynthesis.htm
But: How to bridge the gap between
            morphology and chemistry?

       6CO2 + 6H20  C6H12O6 + 6O2

                            ?


ALT-C
8th September 2010
Nottingham, UK
ALT-C
8th September 2010
Nottingham, UK
ALT-C
8th September 2010
Nottingham, UK
Creating the tutorial
     • eXe eLearning authoring tool
     http://exelearning.org/
     • Jmol 3D molecular browser
     http://jmol.sourceforge.net/
     • Protein Databank source
     http://www.pdb.org/




ALT-C
8th September 2010
Nottingham, UK
Acknowledgments


Keith Johnstone, Fran Tracy, Patrick Carmichael,
Helen Burchmore, Naomi Irvine

                                              Department of
                                              Plant Sciences




  ALT-C
  8th September 2010
  Nottingham, UK               klj33@cam.ac.uk
Acknowledgments


Keith Johnstone, Fran Tracy, Patrick Carmichael,
Helen Burchmore, Naomi Irvine

                                              Department of
                                              Plant Sciences




  ALT-C
  8th September 2010
  Nottingham, UK               klj33@cam.ac.uk

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The Molecular Basis of Photosynthesis

  • 1. The Molecular Basis of Photosynthesis Katy Jordan, University of Cambridge klj33@cam.ac.uk ALT-C 8th September 2010 Background credit: Nottingham, UK Matthew Fang at Flickr
  • 2. Why is it important to understand photosynthesis? • Photosynthesis is fundamental to our past and our future • The most important biochemical process on Earth! • Holds key to sustainable energy? ALT-C 8th September 2010 Nottingham, UK
  • 3. How is the concept of photosynthesis taught? The process by which carbon dioxide is converted to sugar 6CO2 + 6H20  C6H12O6 + 6O2 ALT-C 8th September 2010 Nottingham, UK
  • 4. How is the concept of photosynthesis taught? ALT-C http://www.flickr.com/photos/epsos/3384297473/sizes/m/in/photostream/ 8th September 2010 Nottingham, UK http://school.edturtle.co.uk/photosynthesis.htm
  • 5. But: How to bridge the gap between morphology and chemistry? 6CO2 + 6H20  C6H12O6 + 6O2 ? ALT-C 8th September 2010 Nottingham, UK
  • 8. Creating the tutorial • eXe eLearning authoring tool http://exelearning.org/ • Jmol 3D molecular browser http://jmol.sourceforge.net/ • Protein Databank source http://www.pdb.org/ ALT-C 8th September 2010 Nottingham, UK
  • 9. Acknowledgments Keith Johnstone, Fran Tracy, Patrick Carmichael, Helen Burchmore, Naomi Irvine Department of Plant Sciences ALT-C 8th September 2010 Nottingham, UK klj33@cam.ac.uk
  • 10. Acknowledgments Keith Johnstone, Fran Tracy, Patrick Carmichael, Helen Burchmore, Naomi Irvine Department of Plant Sciences ALT-C 8th September 2010 Nottingham, UK klj33@cam.ac.uk

Notas do Editor

  1. Hello, I’m Katy Jordan, and today I’m going to give a brief introduction to my Jorum competition entry, which is an online tutorial on the molecular basis of photosynthesis.
  2. Why is this important? Photosynthesis is a fundamental process in biology. It’s not really possible to over-state the importance of photosynthesis; it has made life on Earth possible. A deeper understanding of it may also offer potential solutions to reduce atmospheric carbon dioxide, and develop sustainable energy sources.
  3. Teaching photosynthesis typically starts from the general principle, defining it as the process by which carbon dioxide is converted to sugar. This definition can be fleshed out and quantified through the chemical equation of the process; that is, six carbon dioxide and 6 water molecules gives one molecule of glucose and six oxygen molecules.
  4. An understanding of plant morphology is also important; leaves as the site of photosynthesis, and more specifically within them, the specialised organelles called chloroplasts within them containing the all important molecule, chlorophyll.
  5. But, this does not tell the full story. In order to fully understand what is going on, how can we bridge the gap between morphology and chemistry? And by what mechanism does the chemical transformation occur? This is where digital technologies can help.
  6. I’m just going to quickly switch from Powerpoint now to the tool. OPEN SAFARI. The online tutorial aims to give an understanding of what is involved in photosynthesis on the molecular level. This can seem very abstract and intangible as it’s not possible to physically observe in this level of detail in real life. In order to address this, the tool combines a narrative panel to the right, which guides the reader through the process, and embedded radio buttons allow readers to ‘see’ what is happening at the point in an embedded 3D molecule viewer to the left, with the most important structural features highlighted. It is important to be able to view the molecules in 3D because the spatial relationship between them facilitates the process. It is also important to have them set within a narrative to guide the learner; when you approach a new topic you don’t know what it is that you don’t know, so if we had Jmol on its own students may not know where to start.
  7. To give you a quick example of this: here the reader is guide through the transfer of excited electrons through a photosynthetic reaction centre. The light is first absorbed by a pair of chlorophyll molecules, to another, and then on to pheophytin, causing charge separation and creating potential energy, and then demonstrating how the high-energy electron is trasnferred further, and so on.
  8. This format of online tutorial has the potential to be applied to a wide range of teaching scenarios within the biosciences. It was entirely created using Open source and freely available resources; an understanding of writing basic html webpages is required to embed the molecules, but it does not require specialist programming knowledge. Initially I used eXe, which is a tool for creating elearning packages, to create the general structure and navigation system. After this, I manually edited the nest of html pages to embed the 3D molecules using an open-source viewer called Jmol. The files of the 3D molecules themselves which the viewer displays were sourced from the Protein Databank, which is a fantastic online database of 3D crystllographic structures; it currently holds over 67 thousand records and is constantly growing.
  9. Thank-you for listening; if you would like to get in touch about anything relating to the tool or how it was made please do drop me an email, my email address is shown here; and I would just like to take a moment to thank the funders and everyone involved in the project that made this work possible, the Teaching for Learning network funded by the Cambridge-MIT institute. Thank you!