Invitro Screening of Anti-Cancer Drugs.

1 J.K.K. Munirajah Medical Research Foundation College of Pharmacy, B.Komarapalayam, Nammakal. 2 PGP College Of Pharmaceutical Sciences And Institute Nammakal, Tamilnadu

Cancer is a term used for diseases in which abnormal cells divide without control and are able to invade the adjacent and distant tissues from the normal cells of the body. Due to lack of its pathophysiology, the challenging task at this moment is to identify the novel methods which can identify and develop molecules. Even though in vivo models provide more predictable results, the NCI has planned an in vitro screening prior to in vivo testing of a therapeutic agent for reducing the consumption, usage & death of animals and for testing a large number of compounds in small quantities. Those screened compounds will be recommended for the next stage of testing in animals. ,[object Object],[object Object],[object Object],[object Object],[object Object]

PROPERTIES ASSAYS ENZYMATIC PROPERTY MTT ASSAY MEMBRANE INTEGRITY DYE EXCLUSION TEST DNA CONTENT FLUORESCENT ASSAY PROTEIN CONTENT SRB ASSAY CLOGENIC PROPERTY CLOGENIC ASSAY

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5. Centrifuge the plate, discard the supernatant and precipitated formazan salt was dissolved in 100 μl of isopropenol/DMSO. 6. The plate samples were read at 570 nm microtiter plate reader. IC 50 of drugs can be determined by counting the viable cells. % Cell viability Absorbance of treated cells (%MTT reduction) Absorbance of untreated cells 100

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],No. of viable cells Total no of cells (viable+dead) 100 % Cell viability A fluorescent stain used for labeling DNA in which the replicating DNA will incorporate the dye. Resulting fluorescence is measured by flow cytometry/ florescent microscopy.

[object Object],[object Object],[object Object],[object Object],[object Object],such as CLONOGENIC assay which measures the growth inhibiting activity by counting the Tumour colonies of a single cell suspension grown in Agar/methyl cellulose medium. CELL COUNTING assay , by counting the tumor cells using haemocytometer.

Invitro Screening of Anti-Cancer Drugs.

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Invitro Screening of Anti-Cancer Drugs.