9. Grading on Light Microscopy *record actual number of bacilli seen in 100 fields 100 neg No AFB in 100 oil immersion fields 100 Scanty* pos 1-9 AFB/100 oil immersion fields 100 1+ pos 10-99 AFB/100 oil immersion fields 50 2+ pos 1-10 AFB/ oil immersion field 20 3+ pos More than 10 AFB /oil immersion field No. of fields to be examined Grading Result If the slide has
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12. Processing of Sputum Specimens CONVENTIONAL or MOLECULAR METHODS CONVENTIONAL or MOLECULAR METHODS ID AST POSITIVE NEGATIVE CULTURE SOLID or LIQUID CONCENTRATION (Centrifuge) DIGESTION Decontamination Specimen Sputum Collection System SMEAR (Microscopy)
