4. 2007 Yamakana iPSC
1. low reprogramming efficiency OSKM factors pluripotent cell
2006 paper 0.01–0.5% 2007
Yamakana
2006 Fbx15 1/10 0.001–0.03%
factors
Oct4, Sox2, Klf4, and
2. genomic alterations due to viral integration
reverse transcribe OSKM DNA DNA c-Myc
3. c-Myc Klf-4 oncogene
telomere iPSC/piPSC
telomerase (~50 cycles)
ES cell telomerase telomere
2007 Yamanaka Nanog selection marker
iPSC hTERT(human
Telomerase Reverse Transcriptase) hES(human ES)cell
line hES iPSC
60
clone cell
doubling time
• 46.9 ± 12.4 (201B2)
• 47.8 ± 6.6 (201B6)
• 43.2 ± 11.5 (201B7)
•
hES doubling time clones
iPSC
telomere
GFP(Green Fluorescent Protein)
Chimera GFP transfection
Yamanaka paper GFP ”
” GFP Lasker
Nobel Prize
4
5. 2007 stem cell
gene targeting in mice
Bibliography
1. Campbell NA, Reece JB. BIOLOGY. 6th ed. Benjamin
iPSC piPSC
Cummings; 2001.
oncogene Literature
letter PNAS 1. , , , (2008) pp
”Distinguishing Cancerous iPSCs from ESCs” 20-26
iPSC ESC 2. Zhou et al., Generation of Induced Pluripotent Stem Cells
iPSC Using Recombinant Proteins, Cell Stem Cell (2009) pp.
1-4
3. JB Gurdon, The Developmental Capacity of Nuclei taken
from Intestinal Epithelium Cells of Feeding Tadpoles, J.
Cancerous iPSC Embryo. exp. Morph., (1962) pp. 622-40
4. JB Gurdon et al., Nuclei of Adult Mammalian Somatic
Cells Are Directly Reprogrammed to oct-4 Stem Cell
Gene Expression by Amphibian Oocytes, Current
Biology (2003) pp. 1206–1213
5. S Yamanaka et al., Induction of Pluripotent Stem Cells
from Mouse Embryonic and Adult Fibroblast Cultures by
Defined Factors, Cell, (2006) pp. 663-676
6. S Yamanaka et al., Generation of germline-competent
induced pluripotent stem cells, Nature, (2007) pp.
... 313-317
20 60~80 7. JA Thomson, Embryonic Stem Cell Lines Derived from
Human Blastocysts, Science, (1980) pp. 1145-47
8. S Yamanaka et al., Embryonic Stem Cell Lines Derived
from Human Blastocysts, Nature Biotechnology, (2009)
pp. 1-3
9. S Yamanaka et al., Induction of Pluripotent Stem Cells
from Adult Human Fibroblasts by Defined Factors, Cell,
(2007) pp. 861-872
10. JA Thomson et al., Induced Pluripotent Stem Cell Lines
Derived from Human Somatic Cells, Science Express,
(2007) pp. 1-4
11. RM Marion et al., Telomeres Acquire Embryonic Stem
Cell Characteristics in Induced Pluripotent Stem Cells,
Cell Stem Cell, (2009) pp. 141-154
or
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