1. Making a Transgenic Plant

3. Limitations of Agrobacterium -mediated Transgenesis Dicots Tomatoes Potatoes Apples Soybeans Monocots Corn Wheat Agrobacterium only infect dicot plants. So, the alternative is to use a gene gun.

4. http://www.hort.purdue.edu/hort/courses/hort250/animations/Gene%20Gun%20Animation/Genegun1.html Gene Gun animation

5. From an agribusiness standpoint – Both seed stock and pesticide are products from the same company

6. The Flavr Savr ™ Tomato

7. Bt Crops Bacillus thuriengensis is a bacterium that produces a natural protein insect poison (Bt toxin). Upon consumption of the bacteria, the insect dies. This is a “natural” pesticide used by organic farmers

8. Enhancing Vitamin/Mineral Content “ Golden Rice” contains transgenes that allow it to produce vitamin A (beta-carotene) and increase levels of iron This is a humanitarian project designed to combat Vitamin A deficiency

9. Some examples of transgenic animals Why are all these glowing green?

10. Green Fluorescent Protein A gene product of the jellyfish Aequorea victoria . Will fluoresce green when exposed to blue light. This is used as a tracking molecule to study physiological and cellular processes.

11. There are now several different modifications of GFP: “ Enhanced GFP” Blue Cyan Citrine Also fluorecent proteins form other animals such as anemones (RFP) ‏ GloFiish

12. Tetra Rhesus Monkey 1999 2001 Copycat (Cc) ‏ Domestic Cat 2003 Prometea Horse Idaho Gem Mule Pigs Male Mouse Cloned Animals

13. Champion Racehorse “ Royal Blue Moon Too” $150,000 Viagen

14. Cyagra Cloned Beefstock Note: This has now been approved by both the FDA and the USDA

15. Idea – Bring endangered species to term in a surrogate of another species (non-endangered) ‏ Preserving Endangered Species

16. Giant Panda Cloning Cloned panda embryos have been produced in China Difficulty finding suitable surrogate

17. Cloning Extinct Species Would require an intact nucleus Spanish Ibex 2000 West African Black Rhino 2006 Tasmanian Tiger 1936

18. Cloning Extinct Species The Future?

20. Human Cloning May Be Underway... Clonaid – an arm of the Raelian sect claims to have cloned 5 human babies. The location of the offshore laboratory is a secret. Human cloning, in principle, should be easier than that of large animals – since we know much more about human physiology. The only real barrier regulatory...

21. Modified from original power point created by Jeff Reinking, SUNY New Paltz