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Multi-Stage Activated Biological Process
(MSABP™)
Eliminates Excess Sludge
DAVE LAUER, P.E.
VP – SALES & MARKETING
Revisions by JACK AKIN, PE, MS
EMC-Engineers/Scientists, LLC
Topics of Discussion
 MSABP basics
 Evolution of the process
 Principles, design and performance of the
process
 Equipment scope
 Case histories
 Economic advantages
What is MSABP?
 Attached growth biological wastewater treatment process
 Multiple biological selectors in series
 Produces environment for the creation of naturally occurring microbial
food chains
 Whereby the organisms that reduce the organics are in turn
consumed by higher level organisms, termed “vulture filtrators”, and
removed from the flow train.
 Effectively eliminates pollutants in wastewater
 Does so without the generation of waste sludge (biosolids) common in
other biological processes
 Results in substantial capital and operation cost savings
Aeration Clarifier
TYPICAL ACTIVATED SLUDGE PROCESSTYPICAL ACTIVATED SLUDGE PROCESS
Aeration Clarifier
Effluent
Return Sludge
Mixed
Liquor
Screened
Degritted
Influent
Waste Sludge
MULTI-STAGE ACTIVATEDMULTI-STAGE ACTIVATED
BIOLOGICAL PROCESSBIOLOGICAL PROCESS
(MSABP(MSABP®®
))
Screened
Degritted
Influent
Effluent
• Custom designed process with pretreatment consisting of fine screening, and
grit removal
• Removal of inerts creates the required environment for the MSABPTM
reactor
• Process is based on spatial microorganism successions and trophic chains
• Provides for conditions in which organics are consumed by primary
microorganisms while the primary organisms are later consumed by higher
order predatory microbes.
• Process utilizes naturally occurring microbial food chains to reduce excess
sludge
MSABPTM
PROCESS
MSABP COMPLIES WITH
GLUMRB STANDARDS
• BOD LOADING PER UNIT VOLUME
• AERATION SYSTEM AND BLOWER
SIZING BASED ON STANDARDS FOR
BOD and NH3 DEMAND
• PROCESS EFFICIENCY
• The MSABP is an attached growth
process based on selector technology and
the creation of microbial food chains, where
the organisms that reduce the organics are
in turn consumed by higher level organisms,
termed predatory microbes, and removed
from the flow train.
• The hyper plug flow scheme and attached
growth technology produces a high quality
effluent in a simple, easy to operate
package.
• With the MSABP, there is reduced excess
Multi-Stage Activated
Biological Process
(MSABP™)
Who is Aquarius?
 A collection of engineers
and scientists
 250 years combined
wastewater treatment
experience
 Proprietary processes and
equipment
• MSABP
• ELCAT
• Diffused Aeration
 Offices in
• Port Washington, WI
• Israel
Who is EMC?
• A group of civil, environmental and
structural engineers and scientists
• Wastewater treatment systems
designers with over fifty years of
collective experience specific to
MSABP technology
• Offices in Medford and Jacksonville,
Oregon
• In professional cooperation with
Aquarius
- Engineers/Scientists, LLC (a BioScape Technologies Affiliate)
Environmental
Management
Consultants
Grants Pass * Jacksonville * Medford, OR
Ph: 541-474-9434, Ext. 1 * Fax 541-727-5488
bioscapetechnologies@charter.net, emc@emcengineersscientists.com
http://wwwemcengineersscientists.com
Tim Bossard, is a professional Civil
Engineer, is well acquainted with all
aspects of wastewater system and site
development design, and is the
structural authority with respect many
BioScape projects.Tim has over 35
years of civil and structural
engineering experience.
Jack Akin, is an Environmental
Scientist and professional
Environmental Engineer, and, as the
foremost domestic expert on the
biochemical processes associated with
BioScape treatment systems, works as
part of this team to provide the best
service available.
►Dr. Efim Monosov, Chief Technical OfficerDr. Efim Monosov, Chief Technical Officer
 Chair of Civil Engineering, Leningrad University,Chair of Civil Engineering, Leningrad University,
RussiaRussia
►Developed ELCAT as part of doctorateDeveloped ELCAT as part of doctorate
studiesstudies
 Electro-Catalytic treatment of non-Electro-Catalytic treatment of non-
biodegradable wastewaters, and inorganicbiodegradable wastewaters, and inorganic
wastes such as herbicides, dyes, phenolswastes such as herbicides, dyes, phenols
►Established Elif Technology Ltd in 1994Established Elif Technology Ltd in 1994
►Developed MSABPDeveloped MSABP
 Biological treatment of municipal and industrialBiological treatment of municipal and industrial
wastewaters w/o waste sludge generationwastewaters w/o waste sludge generation
TechnologyTechnology
DevelopmentDevelopment
 MSABP utilized dispersedMSABP utilized dispersed
media initially with limitedmedia initially with limited
resultsresults
 Difficult to control biomassDifficult to control biomass
 First generation media usedFirst generation media used
successfully in industrialsuccessfully in industrial
applicationsapplications
 First applied in 1997First applied in 1997
at 30,000 GPD foodat 30,000 GPD food
oil processor foroil processor for
pre-treatmentpre-treatment
 Continued research lead toContinued research lead to
development of modern daydevelopment of modern day
media and processmedia and process
Industrial & Municipal ApplicationsIndustrial & Municipal Applications
….and No
Waste Sludge
MSABP Process
 Custom designed process
with pretreatment consisting
of non-biodegradable
removal
 Removal of non-biodegs
creates the required
environment for the MSABP
reactor
 Process is based on spatial
microorganism
successions and trophic
chains
PrimaryPrimary
VultureVulture
PrimaryPrimary
PrimaryPrimary
MSABP Process
 Provides for conditions in
which organics are
consumed by primary
microorganisms while the
primary organisms are later
consumed by higher
vulture filtrators
 Process utilizes naturally
occurring microbial food
chains to eliminate waste
sludge
PrimaryPrimary
PrimaryPrimary
PrimaryPrimary
VultureVulture
Oxidation and Synthesis:Oxidation and Synthesis:
Organic Matter + OOrganic Matter + O22 + Nutrients + Bacteria+ Nutrients + Bacteria
COCO22 + H+ H22O + (New Bacterial Cells) + EnergyO + (New Bacterial Cells) + Energy
Principal of MSABPPrincipal of MSABP
 Mass quantity of new cells (biomass) produced in aMass quantity of new cells (biomass) produced in a
stage will be lower than mass quantity of raw organicstage will be lower than mass quantity of raw organic
material oxidized in the stagematerial oxidized in the stage
 Energy transferred to the next trophic level results inEnergy transferred to the next trophic level results in
only a fraction being converted to new biomass theonly a fraction being converted to new biomass the
rest going to metabolic processesrest going to metabolic processes
Principle of the Process
Principle of the ProcessPrinciple of the Process
DecreaseinBiomass
DecreaseinBiomass
MassMass
of Finalof Final
ConsumersConsumers
Mass ofMass of
IntermediateIntermediate
ConsumersConsumers
Mass ofMass of
Primary ConsumersPrimary Consumers
Mass of PrimaryMass of Primary
ProducersProducers
DecreaseinEnergy
DecreaseinEnergy
Municipal ApplicationsMunicipal Applications
 ConventionalConventional
 DevelopmentsDevelopments
 SchoolsSchools
 ParksParks
 RetrofitsRetrofits
 NitrificationNitrification
Industrial ApplicationsIndustrial Applications
• FoodFood
• ChemicalChemical
• PharmaceuticalPharmaceutical
• PetrochemicalPetrochemical
• CosmeticCosmetic
• TextileTextile
• SlaughterhouseSlaughterhouse
 Bioreactor is divided intoBioreactor is divided into
8-12 stages8-12 stages
 Each cell contains aEach cell contains a
different and progressivelydifferent and progressively
designed food chaindesigned food chain
environmentenvironment
 Proprietary submergedProprietary submerged
fixed film media is used infixed film media is used in
each stage to provide higheach stage to provide high
bacteria density andbacteria density and
substrate contactsubstrate contact
 Media “stabilizes” desiredMedia “stabilizes” desired
organisms to provide highorganisms to provide high
removal efficiencies withremoval efficiencies with
negligible net sludge yieldnegligible net sludge yield
MSABP Design
Sizing Criteria
 Based on flow, 16-24 hours hydraulic retention time
– Dependent on level of treatment required
– Longer HRT for higher efficiency treatment
 Based on BOD5 loading < 40 #/d/kcf at 8-10°C
– Municipal typically < 15 #/d/kcf at 8-10°C
 8-12 stages
– Dependent on level of treatment required
Stage 1: Logarithmic Growth
 Absorption of dissolved organics by
organisms on the fixed growth media
 Logarithmic biomass growth in a short period
of time
 Carbon adsorption relative to mass of
microorganisms and oxygen
 Significant BOD5 reduction in this phase
Stage 2: Declining Growth
Complex oxidation process begins
thereafter with three kinds of reactions
– Biomass synthesis
– Endogenous respiration
– Nitrification-denitrification
Stage 3: Endogenous Phase
 Oxygen demand decreases in later stages of
the process
 Microorganisms are assimilated by higher
order organisms in the food chain
 Massive destruction of sludge mass occurs
 Process minimizes the “sludge” remaining in
the effluent with the only “sludge” leaving the
system is the TSS meeting permitted limits
Municipal MSABP Performance
MSABP Dissolved Oxygen Gradient
0.2
0.6
1
1.5
2
2.7
3.4
4.1
4.6
4.8
4.9 5
0
1
2
3
4
5
6
Stage
#1
Stage
#2
Stage
#3
Stage
#4
Stage
#5
Stage
#6
Stage
#7
Stage
#8
Stage
#9
Stage
#10
Stage
#11
Stage
#12
MSABP Stages v.s DO
DO,ppm
DO, ppm
Municipal MSABP Performance
MSABP Kinetic
0
50
100
150
200
250
2 4 6 8 10 12 14 16 18 20 22 24
HRT, hours
Concentration,ppm
BOD
TSS
NH4
Aquarius MSABP Scope of Supply
 Inert removal
 Diffused aeration
 Fixed growth capture media
 Aeration blowers
 Instrumentation
 System process controls
Inert Removal
 Removal of inert material
 Pretreatment screening
and grit removal
 Fine screen, 1 mm
openings
 Standard grit removal for
municipal applications
 Alternative grit trap or
septic tank pretreatment
Mechanical Purification
 Depending on characteristics,
options range from simple
settling tanks to mechanical
purification systems for
elimination of sand and coarse
mechanical impurities with
settling velocity exceeding 20
mm/s (about 4 fpm).
 Various designs for bar
screening configurations exist to
remove other non-organic
materials.
Diffused Aeration System
 Fine or coarse bubble
diffused aeration
 Oxygen provided to
satisfy process demand
in aerobic cells
 Mixing agitation to
control biomass film on
media
Fixed Growth Media and Frames
 Polyamide &
polypropylene material
 Ultra high media
surface area
 Uniform density to
support population
 Support frames are 304
stainless steel
 Integral rack covers or
basin covers
Aeration Blowers
 Duty and standby
blower configuration
 Positive displacement
typical
 Centrifugal for larger
flow applications
Instrumentation & Control
 Instrumentation
– Dissolved oxygen
 Control
– Overall oxygen gradient
– Blower output feedback
Influent Effluent
Flow(gpd)
210,000
---
HRT(hrs) 16 ---
BOD(ppm) 350 < 15
TSS(ppm) 400 < 20
NH3(ppm) 70 < 1
Yavne WWTP
Affidavit & 1 Year Data
Influent Effluent
COD(ppm) 1,000 <60
BOD(ppm) 450 <10
TSS(ppm) 250 <10
NH3(ppm) 100 <1
TN (ppm) --- < 5
JH Ranch, Etna, CA
45,000 GPD
Influent Effluent
Flow(gpd) 5,000 ---
HRT(hrs) 20 ---
BOD(ppm) 200 <5
TSS(ppm) 180 <5
NH3(ppm) 20 <0.1
TN (ppm) --- <3
Northern Moraine Utility Commission
Glenbeulah, WI
Northern Moraine Utility Commission
Northern Moraine Utility
Commission
Northern Moraine Utility Commission
 Consistent performance
 Cold wastewater
compatible
 Single digit effluent
 Total nitrogen removal
 Grit removal important
MSABPTM
SYSTEM COMPONENTS
 Fine screening & grit removal
 Diffused aeration
 Fixed growth capture media
 Aeration blowers
 Instrumentation
SCREENING AND GRIT
REMOVAL
 Pretreatment screening
and grit removal
 Fine screen, 1 mm
openings rotary drum
style.
 Internally fed rotary drum
preferred.
 Standard grit removal for
municipal applications
 Removal of inerts
Headworks
 Generate 6-8 ft3
screenings per MG
 30-40% solids
 Relatively stable
 Landfill disposal
DIFFUSED AERATION SYSTEM
 Fine pore or coarse
bubble diffused
aeration
 Oxygen provided to
satisfy process
demand in all stages
 Aerated mixing
controls biological
attachment
FIXED GROWTH MEDIA
AND FRAMES
 Polyamide & polypropylene
material
 0.25 #media/ft3
of reactor
volume
 Uniform density to support
population
 Support frames are 304
stainless steel
 Integral covers or basin
AERATION BLOWERS
 Duty and standby
blower configuration
 Positive
displacement with
sound enclosure
INSTRUMENTATION
AND CONTROL
 Instrumentation
– Dissolved Oxygen
– Optical Probes our choice
 Control
– Oxygen in first stage
– Check mid point
– Blower output feedback
TYPICAL MUNICIPAL
MSABP Dissolved Oxygen Gradient
0.2
0.6
1
1.5
2
2.7
3.4
4.1
4.6
4.8
4.9 5
Stage
#1
Stage
#2
Stage
#3
Stage
#4
Stage
#5
Stage
#6
Stage
#7
Stage
#8
Stage
#9
Stage
#10
Stage
#11
Stage
#12
MSABP Stages v.s DO
DO, ppm
SYSTEM PERFORMANCE
Typical Removal Efficiencies
BOD5 95 – 99%
COD 85 – 95%
TSS 95 – 97%
NH3 90 – 99%
PHOSPHORUS REMOVAL
 No Bio-P with MSABPTM
process
 Alum or ferric addition following last stage, 10
min HRT
 Precipitation Stage for 2 hr HRT
 1 mg/l
 Filtration for fractional P
TYPICAL PROJECTS
Sharon Labs
Fine Chemistry
Israel
Prosintex
Pharmaceutical
Italy
Teva
Pharmaceuticals
Israel
Veolia
(Lipodan) Oil
Israel
Eisen Lubeo
Food Oil
China
Gadot
Chemicals
Israel
JH Ranch
Municipal
California – USA
Johnson & Johnson
Cosmetics
China
Kibbutz Yavne
Municipal
Israel
Tifico
Textile
Indonesia
JH RANCH, ETNA, CA
40,000 GPD
Influent Effluent
pH 6.2-7.5 6.8-7.4
COD(ppm) <1,000 <60
BOD(ppm) <450 <10
TSS(ppm) <250 <10
N-NH3(ppm) <100 <1.0
TEVA PHARMACEUTICAL,
ISRAEL 80,000 GPD
Influent Effluent
pH 6.0-9.0 6.0-9.0
COD(ppm) <50,000 <6,000
BOD(ppm) <20,000 <1,000
TSS(ppm) <2,000 <350
TOC(ppm) <15,000 <1,500
JOHNSON & JOHNSON, CHINA
13,000 GPD
Influent Effluent
pH 5.6-7.2 5.6-7.2
COD(ppm) <3,000 <300
BOD(ppm) <1,000 <150
FOG(ppm) <30 <10
LAS(ppm) <100 <10
LINEAR ALKYLBENZENE SULFONATE , BIODEGRADABLE SURFACTANT
PRETREATMENT
GLASS MANUFACTURER
STAGE 1
STAGE 4
STAGE 12
O-I GLASS
Influent
Effluent
Influent Effluent
BOD(ppm) <700 <15
TSS(ppm) <300 <40
O&G(ppm) <150 <5
PORTABLE TREATMENT UNIT
NORTHERN MORAINE UTILITY
STAGES 1, 6 AND 12
INFLUENT STAGE 6 EFFLUENT
NMUC WWTP Influent Characteristics
0
10
20
30
40
50
60
70
80
90
100
110
120
130
140
150
160
170
180
190
200
210
220
230
240
250
3/18
3/22
3/25
3/29
4/1
4/5
4/8
4/12
4/15
4/19
Sample Date
Concentration(mg/l)
INF BOD
INF SS
INF NH3
INF TKN
NMUC WWTP Effluent Performance
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
3/18 3/22 3/25 3/29 4/1 4/5 4/8 4/12 4/15 4/19
Sample Date
Concentration(mg/l)
EFF BOD
EFF SS
EFF TN
EFF TKN
EFF NH3
COLD WEATHER PERFORMANCE
STAGE 1 FREE SWIMMER &
BEGGIATOA
STAGE 4 - BRISTLEWORM
STAGE 6 - GASTROTICH
STAGE 10 - ROTIFER
WAL-MART SUPER CENTER
BEFORE AND AFTER
Influent Effluent
BOD(ppm) 2520 86
TSS(ppm) 1560 200
O&G(ppm) 63 6
FINE SCREENING
KIBBUTZ KVUZAT YAVNE,
ISRAEL 210,000 GPD
Influent Effluent
pH 6.0-8.0 6.0-9.0
BOD(ppm) <300 <20
TSS(ppm) <100 <30
N-NH3(ppm) <80 <1.0
PERFORMANCE PER STAGE
MSABP Kinetic
0
50
100
150
200
250
300
350
400
450
2 4 6 8 10 12 14 16
HRT, hours
Concentration,ppm
BOD
TSS
NH4
Yavne WWTP Performance Data
0
100
200
300
400
500
600
700
800
900
1,000
1,100
1,200
7.06.06
15.06.06
21.06.0626.06.06
6.07.06
13.07.06
25.07.06
30.07.06
7.08.06
16.08.06
29.08.06
11.09.06
19.09.06
5.10.06
18.10.06
6.11.06
15.11.06
23.11.06
10.12.06
4.01.07
30.01.0725.02.07
22.03.07
20.06.07
Sample Date
Concentrationppm
Influent COD ppm
Effluent COD ppm
Parameter Average Influent (ppm) Average Effluent (ppm)
COD 538 83
Yavne WWTP Performance Data
0
20
40
60
80
100
120
140
160
180
200
220
240
260
280
300
320
340
360
380
400
420
440
460
480
500
520
540
7.06.06
15.06.06
25.06.06
4.07.06
11.07.06
23.07.06
27.07.06
3.08.06
13.08.06
20.08.06
11.09.06
27.09.06
12.10.06
22.10.06
9.11.06
16.11.06
30.11.06
4.01.07
30.01.07
25.02.07
22.03.07
20.06.07
Sample Date
Concentrationppm
Influent TSS ppm
Effluent TSS ppm
Parameter Average Influent (ppm) Average Effluent (ppm)
TSS 186 19
Yavne WWTP Performance Data
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
7.06.06
13.06.06
21.06.06
28.06.064.07.067.08.068.08.06
13.08.06
16.08.06
17.08.06
20.08.06
29.08.065.09.06
11.09.06
14.09.06
17.09.06
31.10.066.11.069.11.06
12.11.06
15.11.06
16.11.06
19.11.06
23.11.06
30.11.06
10.12.06
25.12.06
31.12.064.01.078.02.07
Sample Date
Concentrationppm
Influent NH3 ppm
Effluent NH3ppm
Parameter Average Influent (ppm) Average Effluent (ppm)
NH3 55 0.69
PORT WASHINGTON, WI
Influent vs Effluent
Port Washington
MSABP Pilot
0
25
50
75
100
125
150
175
200
225
250
275
300
2/25/09
3/3/09
3/10/09
3/17/09
3/24/09
3/31/09
4/7/09
4/14/09
4/22/09
4/29/09
5/4/09
mg/L
influent ammonia influent BOD influent TSS influent total P effluent ammonia effluent BOD effluent TSS effluent total P
TEMPERATURE
Cell #1 water temp
Port Washington MSABP
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
16.0
17.0
18.0
19.0
20.0
21.0
22.0
2/16/092/20/092/26/093/4/093/10/093/16/093/20/093/26/094/1/094/7/094/14/094/20/094/24/094/30/095/6/095/12/095/18/095/22/095/29/096/4/096/10/096/16/096/20/096/25/097/1/097/8/097/14/098/6/098/12/098/19/098/25/099/1/099/8/09
11/16/09
11/20/09
11/30/0912/4/09
12/10/09
12/16/09
12/29/091/8/101/15/101/21/10
Temp(deg.C)
water temp
NTU FEBRUARY 2009
NTU
Port Washington
MSABP Pilot Unit
February
0
1
2
3
4
5
6
7
8
9
10
2/15/09
2/16/09
2/17/09
2/18/09
2/19/09
2/20/09
2/21/09
2/22/09
2/23/09
2/24/09
2/25/09
2/26/09
2/27/09
2/28/09
3/1/09
3/2/09
NTU
AS A RESULT
 JENNINGS NORTHWEST
REGIONAL UTILITY
SELECTS MSABP.
 UPGRADE FROM CURRENT SBR TECHNOLOGY
 0.35 – 1.7 MGD CAPACITY
 FEBRUARY 2011 STARTUP
PLANT EFFLUENT
NMUC IN OPERATION
NEW HEADWORKS
INFLUENT EQ
STAGE 1, STAGE 6
STAGE 12
MSABP SUMMARY
 Fine screening, long HRT, hyper plug flow, selector technology
with discrete spatial zones and attached growth allow the
organisms to function without waste sludge production.
 Operational control is simple and automatic
 Stable
 Cost Effective and Beneficial Process
UNDER CONSTRUCTION
 ROUNDUP RIVER RANCH, CO
 WAYNE, NE
 MANCOS, CO
 TORTILLA KING, KS
 DRIGGS, ID
Influent Effluent
Flow(gpd) 4,000 ---
HRT(hrs) 12-24 ---
BOD(ppm) 175 <10
TSS(ppm) 200 <10
NH3 (ppm) 30 <1
TN (ppm) 60 <10
Roselle, Illinois - Devlin WWTP
Roselle, Illinois
 “…roughly $100,000 annually
to haul its sludge…”
 “…estimated it would save $9
million…”
Effluent Turbidity
NH3N TN
Influent 28.3 57.2
Stage 2 18.1 36.6
Stage 4 0.346 20.0
Stage 6 0.031 13.2
Stage 8 0.031 9.2
Stage 10 0.027 7.9
Stage 12 0.021 4.3
Nitrogen Balance
Influent Effluent
Flow(gpd) 25,000 ---
HRT(hrs) 16 ---
BOD(ppm) 650 <225
TSS(ppm) 650 <225
Wal-Mart, Alpharetta, GA
25,000 GPD Pretreatment Facility
Influent Effluent
Flow(gpd) 85,000 ---
HRT(hrs) 24 ---
BOD(ppm
)
300 <25
TSS(ppm) 300 <35
NH3(ppm) 40 <1
TN (ppm) --- <15
Villarrin de Campos - Zamora, Spain
 Retrofit
 Improved performance
Villarrin de Campos - Zamora, Spain
MSABP Operations
Savings Per MGD
1,900 #/day waste sludge generation
Capital Cost
20 day storage digester (450,000 gal tank @ $600 yd3
concrete) $200,000
Aerobic digester aeration system $50,000
Aerobic digester blower system $75,000
Belt press, polymer feed system $150,000
Building, HVAC, etc. $100,000
Total $575,000
Annually
Mixing power (30 scfm/kcf @ $0.07 / kw-hr) $35,000
Polymer ($12/ ton dry solids) $4,000
WAS disposal (20% conc. @ $37 / wet ton) $65,000
Total $104,000
20 Year Present Worth Savings*
$1,567,100
* 7% discount rate
Contact Information
 Contact: Jack Akin, MS, PE
 Cell Phone: 541-474-9434,
Ext. 1
 Email:
bioscapetechnologies@charter.net
 emc@emcengineersscientists.com
 Jacksonville Office Address:
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Multi-Stage Activated Biological Process (MSABP™)

  • 1. Multi-Stage Activated Biological Process (MSABP™) Eliminates Excess Sludge DAVE LAUER, P.E. VP – SALES & MARKETING Revisions by JACK AKIN, PE, MS EMC-Engineers/Scientists, LLC
  • 2. Topics of Discussion  MSABP basics  Evolution of the process  Principles, design and performance of the process  Equipment scope  Case histories  Economic advantages
  • 3. What is MSABP?  Attached growth biological wastewater treatment process  Multiple biological selectors in series  Produces environment for the creation of naturally occurring microbial food chains  Whereby the organisms that reduce the organics are in turn consumed by higher level organisms, termed “vulture filtrators”, and removed from the flow train.  Effectively eliminates pollutants in wastewater  Does so without the generation of waste sludge (biosolids) common in other biological processes  Results in substantial capital and operation cost savings
  • 4. Aeration Clarifier TYPICAL ACTIVATED SLUDGE PROCESSTYPICAL ACTIVATED SLUDGE PROCESS Aeration Clarifier Effluent Return Sludge Mixed Liquor Screened Degritted Influent Waste Sludge
  • 5. MULTI-STAGE ACTIVATEDMULTI-STAGE ACTIVATED BIOLOGICAL PROCESSBIOLOGICAL PROCESS (MSABP(MSABP®® )) Screened Degritted Influent Effluent
  • 6. • Custom designed process with pretreatment consisting of fine screening, and grit removal • Removal of inerts creates the required environment for the MSABPTM reactor • Process is based on spatial microorganism successions and trophic chains • Provides for conditions in which organics are consumed by primary microorganisms while the primary organisms are later consumed by higher order predatory microbes. • Process utilizes naturally occurring microbial food chains to reduce excess sludge MSABPTM PROCESS
  • 7. MSABP COMPLIES WITH GLUMRB STANDARDS • BOD LOADING PER UNIT VOLUME • AERATION SYSTEM AND BLOWER SIZING BASED ON STANDARDS FOR BOD and NH3 DEMAND • PROCESS EFFICIENCY
  • 8. • The MSABP is an attached growth process based on selector technology and the creation of microbial food chains, where the organisms that reduce the organics are in turn consumed by higher level organisms, termed predatory microbes, and removed from the flow train. • The hyper plug flow scheme and attached growth technology produces a high quality effluent in a simple, easy to operate package. • With the MSABP, there is reduced excess
  • 10. Who is Aquarius?  A collection of engineers and scientists  250 years combined wastewater treatment experience  Proprietary processes and equipment • MSABP • ELCAT • Diffused Aeration  Offices in • Port Washington, WI • Israel
  • 11. Who is EMC? • A group of civil, environmental and structural engineers and scientists • Wastewater treatment systems designers with over fifty years of collective experience specific to MSABP technology • Offices in Medford and Jacksonville, Oregon • In professional cooperation with Aquarius - Engineers/Scientists, LLC (a BioScape Technologies Affiliate) Environmental Management Consultants Grants Pass * Jacksonville * Medford, OR Ph: 541-474-9434, Ext. 1 * Fax 541-727-5488 bioscapetechnologies@charter.net, emc@emcengineersscientists.com http://wwwemcengineersscientists.com
  • 12. Tim Bossard, is a professional Civil Engineer, is well acquainted with all aspects of wastewater system and site development design, and is the structural authority with respect many BioScape projects.Tim has over 35 years of civil and structural engineering experience. Jack Akin, is an Environmental Scientist and professional Environmental Engineer, and, as the foremost domestic expert on the biochemical processes associated with BioScape treatment systems, works as part of this team to provide the best service available.
  • 13. ►Dr. Efim Monosov, Chief Technical OfficerDr. Efim Monosov, Chief Technical Officer  Chair of Civil Engineering, Leningrad University,Chair of Civil Engineering, Leningrad University, RussiaRussia ►Developed ELCAT as part of doctorateDeveloped ELCAT as part of doctorate studiesstudies  Electro-Catalytic treatment of non-Electro-Catalytic treatment of non- biodegradable wastewaters, and inorganicbiodegradable wastewaters, and inorganic wastes such as herbicides, dyes, phenolswastes such as herbicides, dyes, phenols ►Established Elif Technology Ltd in 1994Established Elif Technology Ltd in 1994 ►Developed MSABPDeveloped MSABP  Biological treatment of municipal and industrialBiological treatment of municipal and industrial wastewaters w/o waste sludge generationwastewaters w/o waste sludge generation
  • 14. TechnologyTechnology DevelopmentDevelopment  MSABP utilized dispersedMSABP utilized dispersed media initially with limitedmedia initially with limited resultsresults  Difficult to control biomassDifficult to control biomass  First generation media usedFirst generation media used successfully in industrialsuccessfully in industrial applicationsapplications  First applied in 1997First applied in 1997 at 30,000 GPD foodat 30,000 GPD food oil processor foroil processor for pre-treatmentpre-treatment  Continued research lead toContinued research lead to development of modern daydevelopment of modern day media and processmedia and process
  • 15. Industrial & Municipal ApplicationsIndustrial & Municipal Applications ….and No Waste Sludge
  • 16. MSABP Process  Custom designed process with pretreatment consisting of non-biodegradable removal  Removal of non-biodegs creates the required environment for the MSABP reactor  Process is based on spatial microorganism successions and trophic chains PrimaryPrimary VultureVulture PrimaryPrimary PrimaryPrimary
  • 17. MSABP Process  Provides for conditions in which organics are consumed by primary microorganisms while the primary organisms are later consumed by higher vulture filtrators  Process utilizes naturally occurring microbial food chains to eliminate waste sludge PrimaryPrimary PrimaryPrimary PrimaryPrimary VultureVulture
  • 18. Oxidation and Synthesis:Oxidation and Synthesis: Organic Matter + OOrganic Matter + O22 + Nutrients + Bacteria+ Nutrients + Bacteria COCO22 + H+ H22O + (New Bacterial Cells) + EnergyO + (New Bacterial Cells) + Energy Principal of MSABPPrincipal of MSABP  Mass quantity of new cells (biomass) produced in aMass quantity of new cells (biomass) produced in a stage will be lower than mass quantity of raw organicstage will be lower than mass quantity of raw organic material oxidized in the stagematerial oxidized in the stage  Energy transferred to the next trophic level results inEnergy transferred to the next trophic level results in only a fraction being converted to new biomass theonly a fraction being converted to new biomass the rest going to metabolic processesrest going to metabolic processes
  • 19. Principle of the Process
  • 20. Principle of the ProcessPrinciple of the Process DecreaseinBiomass DecreaseinBiomass MassMass of Finalof Final ConsumersConsumers Mass ofMass of IntermediateIntermediate ConsumersConsumers Mass ofMass of Primary ConsumersPrimary Consumers Mass of PrimaryMass of Primary ProducersProducers DecreaseinEnergy DecreaseinEnergy
  • 21. Municipal ApplicationsMunicipal Applications  ConventionalConventional  DevelopmentsDevelopments  SchoolsSchools  ParksParks  RetrofitsRetrofits  NitrificationNitrification
  • 22. Industrial ApplicationsIndustrial Applications • FoodFood • ChemicalChemical • PharmaceuticalPharmaceutical • PetrochemicalPetrochemical • CosmeticCosmetic • TextileTextile • SlaughterhouseSlaughterhouse
  • 23.  Bioreactor is divided intoBioreactor is divided into 8-12 stages8-12 stages  Each cell contains aEach cell contains a different and progressivelydifferent and progressively designed food chaindesigned food chain environmentenvironment  Proprietary submergedProprietary submerged fixed film media is used infixed film media is used in each stage to provide higheach stage to provide high bacteria density andbacteria density and substrate contactsubstrate contact  Media “stabilizes” desiredMedia “stabilizes” desired organisms to provide highorganisms to provide high removal efficiencies withremoval efficiencies with negligible net sludge yieldnegligible net sludge yield
  • 24. MSABP Design Sizing Criteria  Based on flow, 16-24 hours hydraulic retention time – Dependent on level of treatment required – Longer HRT for higher efficiency treatment  Based on BOD5 loading < 40 #/d/kcf at 8-10°C – Municipal typically < 15 #/d/kcf at 8-10°C  8-12 stages – Dependent on level of treatment required
  • 25. Stage 1: Logarithmic Growth  Absorption of dissolved organics by organisms on the fixed growth media  Logarithmic biomass growth in a short period of time  Carbon adsorption relative to mass of microorganisms and oxygen  Significant BOD5 reduction in this phase
  • 26. Stage 2: Declining Growth Complex oxidation process begins thereafter with three kinds of reactions – Biomass synthesis – Endogenous respiration – Nitrification-denitrification
  • 27. Stage 3: Endogenous Phase  Oxygen demand decreases in later stages of the process  Microorganisms are assimilated by higher order organisms in the food chain  Massive destruction of sludge mass occurs  Process minimizes the “sludge” remaining in the effluent with the only “sludge” leaving the system is the TSS meeting permitted limits
  • 28. Municipal MSABP Performance MSABP Dissolved Oxygen Gradient 0.2 0.6 1 1.5 2 2.7 3.4 4.1 4.6 4.8 4.9 5 0 1 2 3 4 5 6 Stage #1 Stage #2 Stage #3 Stage #4 Stage #5 Stage #6 Stage #7 Stage #8 Stage #9 Stage #10 Stage #11 Stage #12 MSABP Stages v.s DO DO,ppm DO, ppm
  • 29. Municipal MSABP Performance MSABP Kinetic 0 50 100 150 200 250 2 4 6 8 10 12 14 16 18 20 22 24 HRT, hours Concentration,ppm BOD TSS NH4
  • 30. Aquarius MSABP Scope of Supply  Inert removal  Diffused aeration  Fixed growth capture media  Aeration blowers  Instrumentation  System process controls
  • 31. Inert Removal  Removal of inert material  Pretreatment screening and grit removal  Fine screen, 1 mm openings  Standard grit removal for municipal applications  Alternative grit trap or septic tank pretreatment
  • 32. Mechanical Purification  Depending on characteristics, options range from simple settling tanks to mechanical purification systems for elimination of sand and coarse mechanical impurities with settling velocity exceeding 20 mm/s (about 4 fpm).  Various designs for bar screening configurations exist to remove other non-organic materials.
  • 33. Diffused Aeration System  Fine or coarse bubble diffused aeration  Oxygen provided to satisfy process demand in aerobic cells  Mixing agitation to control biomass film on media
  • 34. Fixed Growth Media and Frames  Polyamide & polypropylene material  Ultra high media surface area  Uniform density to support population  Support frames are 304 stainless steel  Integral rack covers or basin covers
  • 35. Aeration Blowers  Duty and standby blower configuration  Positive displacement typical  Centrifugal for larger flow applications
  • 36. Instrumentation & Control  Instrumentation – Dissolved oxygen  Control – Overall oxygen gradient – Blower output feedback
  • 37. Influent Effluent Flow(gpd) 210,000 --- HRT(hrs) 16 --- BOD(ppm) 350 < 15 TSS(ppm) 400 < 20 NH3(ppm) 70 < 1 Yavne WWTP
  • 38. Affidavit & 1 Year Data
  • 39. Influent Effluent COD(ppm) 1,000 <60 BOD(ppm) 450 <10 TSS(ppm) 250 <10 NH3(ppm) 100 <1 TN (ppm) --- < 5 JH Ranch, Etna, CA 45,000 GPD
  • 40. Influent Effluent Flow(gpd) 5,000 --- HRT(hrs) 20 --- BOD(ppm) 200 <5 TSS(ppm) 180 <5 NH3(ppm) 20 <0.1 TN (ppm) --- <3 Northern Moraine Utility Commission Glenbeulah, WI
  • 43. Northern Moraine Utility Commission  Consistent performance  Cold wastewater compatible  Single digit effluent  Total nitrogen removal  Grit removal important
  • 44. MSABPTM SYSTEM COMPONENTS  Fine screening & grit removal  Diffused aeration  Fixed growth capture media  Aeration blowers  Instrumentation
  • 45. SCREENING AND GRIT REMOVAL  Pretreatment screening and grit removal  Fine screen, 1 mm openings rotary drum style.  Internally fed rotary drum preferred.  Standard grit removal for municipal applications  Removal of inerts
  • 46. Headworks  Generate 6-8 ft3 screenings per MG  30-40% solids  Relatively stable  Landfill disposal
  • 47. DIFFUSED AERATION SYSTEM  Fine pore or coarse bubble diffused aeration  Oxygen provided to satisfy process demand in all stages  Aerated mixing controls biological attachment
  • 48. FIXED GROWTH MEDIA AND FRAMES  Polyamide & polypropylene material  0.25 #media/ft3 of reactor volume  Uniform density to support population  Support frames are 304 stainless steel  Integral covers or basin
  • 49. AERATION BLOWERS  Duty and standby blower configuration  Positive displacement with sound enclosure
  • 50. INSTRUMENTATION AND CONTROL  Instrumentation – Dissolved Oxygen – Optical Probes our choice  Control – Oxygen in first stage – Check mid point – Blower output feedback
  • 51. TYPICAL MUNICIPAL MSABP Dissolved Oxygen Gradient 0.2 0.6 1 1.5 2 2.7 3.4 4.1 4.6 4.8 4.9 5 Stage #1 Stage #2 Stage #3 Stage #4 Stage #5 Stage #6 Stage #7 Stage #8 Stage #9 Stage #10 Stage #11 Stage #12 MSABP Stages v.s DO DO, ppm
  • 52. SYSTEM PERFORMANCE Typical Removal Efficiencies BOD5 95 – 99% COD 85 – 95% TSS 95 – 97% NH3 90 – 99%
  • 53. PHOSPHORUS REMOVAL  No Bio-P with MSABPTM process  Alum or ferric addition following last stage, 10 min HRT  Precipitation Stage for 2 hr HRT  1 mg/l  Filtration for fractional P
  • 54. TYPICAL PROJECTS Sharon Labs Fine Chemistry Israel Prosintex Pharmaceutical Italy Teva Pharmaceuticals Israel Veolia (Lipodan) Oil Israel Eisen Lubeo Food Oil China Gadot Chemicals Israel JH Ranch Municipal California – USA Johnson & Johnson Cosmetics China Kibbutz Yavne Municipal Israel Tifico Textile Indonesia
  • 55. JH RANCH, ETNA, CA 40,000 GPD Influent Effluent pH 6.2-7.5 6.8-7.4 COD(ppm) <1,000 <60 BOD(ppm) <450 <10 TSS(ppm) <250 <10 N-NH3(ppm) <100 <1.0
  • 56. TEVA PHARMACEUTICAL, ISRAEL 80,000 GPD Influent Effluent pH 6.0-9.0 6.0-9.0 COD(ppm) <50,000 <6,000 BOD(ppm) <20,000 <1,000 TSS(ppm) <2,000 <350 TOC(ppm) <15,000 <1,500
  • 57. JOHNSON & JOHNSON, CHINA 13,000 GPD Influent Effluent pH 5.6-7.2 5.6-7.2 COD(ppm) <3,000 <300 BOD(ppm) <1,000 <150 FOG(ppm) <30 <10 LAS(ppm) <100 <10 LINEAR ALKYLBENZENE SULFONATE , BIODEGRADABLE SURFACTANT
  • 58.
  • 61. O-I GLASS Influent Effluent Influent Effluent BOD(ppm) <700 <15 TSS(ppm) <300 <40 O&G(ppm) <150 <5
  • 63. STAGES 1, 6 AND 12
  • 64. INFLUENT STAGE 6 EFFLUENT
  • 65. NMUC WWTP Influent Characteristics 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 3/18 3/22 3/25 3/29 4/1 4/5 4/8 4/12 4/15 4/19 Sample Date Concentration(mg/l) INF BOD INF SS INF NH3 INF TKN NMUC WWTP Effluent Performance 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 3/18 3/22 3/25 3/29 4/1 4/5 4/8 4/12 4/15 4/19 Sample Date Concentration(mg/l) EFF BOD EFF SS EFF TN EFF TKN EFF NH3 COLD WEATHER PERFORMANCE
  • 66. STAGE 1 FREE SWIMMER & BEGGIATOA
  • 67. STAGE 4 - BRISTLEWORM
  • 68. STAGE 6 - GASTROTICH
  • 69. STAGE 10 - ROTIFER
  • 71. BEFORE AND AFTER Influent Effluent BOD(ppm) 2520 86 TSS(ppm) 1560 200 O&G(ppm) 63 6
  • 73.
  • 74. KIBBUTZ KVUZAT YAVNE, ISRAEL 210,000 GPD Influent Effluent pH 6.0-8.0 6.0-9.0 BOD(ppm) <300 <20 TSS(ppm) <100 <30 N-NH3(ppm) <80 <1.0
  • 75. PERFORMANCE PER STAGE MSABP Kinetic 0 50 100 150 200 250 300 350 400 450 2 4 6 8 10 12 14 16 HRT, hours Concentration,ppm BOD TSS NH4
  • 76. Yavne WWTP Performance Data 0 100 200 300 400 500 600 700 800 900 1,000 1,100 1,200 7.06.06 15.06.06 21.06.0626.06.06 6.07.06 13.07.06 25.07.06 30.07.06 7.08.06 16.08.06 29.08.06 11.09.06 19.09.06 5.10.06 18.10.06 6.11.06 15.11.06 23.11.06 10.12.06 4.01.07 30.01.0725.02.07 22.03.07 20.06.07 Sample Date Concentrationppm Influent COD ppm Effluent COD ppm Parameter Average Influent (ppm) Average Effluent (ppm) COD 538 83
  • 77. Yavne WWTP Performance Data 0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 7.06.06 15.06.06 25.06.06 4.07.06 11.07.06 23.07.06 27.07.06 3.08.06 13.08.06 20.08.06 11.09.06 27.09.06 12.10.06 22.10.06 9.11.06 16.11.06 30.11.06 4.01.07 30.01.07 25.02.07 22.03.07 20.06.07 Sample Date Concentrationppm Influent TSS ppm Effluent TSS ppm Parameter Average Influent (ppm) Average Effluent (ppm) TSS 186 19
  • 78. Yavne WWTP Performance Data 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 7.06.06 13.06.06 21.06.06 28.06.064.07.067.08.068.08.06 13.08.06 16.08.06 17.08.06 20.08.06 29.08.065.09.06 11.09.06 14.09.06 17.09.06 31.10.066.11.069.11.06 12.11.06 15.11.06 16.11.06 19.11.06 23.11.06 30.11.06 10.12.06 25.12.06 31.12.064.01.078.02.07 Sample Date Concentrationppm Influent NH3 ppm Effluent NH3ppm Parameter Average Influent (ppm) Average Effluent (ppm) NH3 55 0.69
  • 80. Influent vs Effluent Port Washington MSABP Pilot 0 25 50 75 100 125 150 175 200 225 250 275 300 2/25/09 3/3/09 3/10/09 3/17/09 3/24/09 3/31/09 4/7/09 4/14/09 4/22/09 4/29/09 5/4/09 mg/L influent ammonia influent BOD influent TSS influent total P effluent ammonia effluent BOD effluent TSS effluent total P
  • 81. TEMPERATURE Cell #1 water temp Port Washington MSABP 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0 22.0 2/16/092/20/092/26/093/4/093/10/093/16/093/20/093/26/094/1/094/7/094/14/094/20/094/24/094/30/095/6/095/12/095/18/095/22/095/29/096/4/096/10/096/16/096/20/096/25/097/1/097/8/097/14/098/6/098/12/098/19/098/25/099/1/099/8/09 11/16/09 11/20/09 11/30/0912/4/09 12/10/09 12/16/09 12/29/091/8/101/15/101/21/10 Temp(deg.C) water temp
  • 82. NTU FEBRUARY 2009 NTU Port Washington MSABP Pilot Unit February 0 1 2 3 4 5 6 7 8 9 10 2/15/09 2/16/09 2/17/09 2/18/09 2/19/09 2/20/09 2/21/09 2/22/09 2/23/09 2/24/09 2/25/09 2/26/09 2/27/09 2/28/09 3/1/09 3/2/09 NTU
  • 83. AS A RESULT  JENNINGS NORTHWEST REGIONAL UTILITY SELECTS MSABP.  UPGRADE FROM CURRENT SBR TECHNOLOGY  0.35 – 1.7 MGD CAPACITY  FEBRUARY 2011 STARTUP
  • 84.
  • 85.
  • 86.
  • 87.
  • 88.
  • 90.
  • 96. MSABP SUMMARY  Fine screening, long HRT, hyper plug flow, selector technology with discrete spatial zones and attached growth allow the organisms to function without waste sludge production.  Operational control is simple and automatic  Stable  Cost Effective and Beneficial Process
  • 97. UNDER CONSTRUCTION  ROUNDUP RIVER RANCH, CO  WAYNE, NE  MANCOS, CO  TORTILLA KING, KS  DRIGGS, ID
  • 98. Influent Effluent Flow(gpd) 4,000 --- HRT(hrs) 12-24 --- BOD(ppm) 175 <10 TSS(ppm) 200 <10 NH3 (ppm) 30 <1 TN (ppm) 60 <10 Roselle, Illinois - Devlin WWTP
  • 99. Roselle, Illinois  “…roughly $100,000 annually to haul its sludge…”  “…estimated it would save $9 million…”
  • 101. NH3N TN Influent 28.3 57.2 Stage 2 18.1 36.6 Stage 4 0.346 20.0 Stage 6 0.031 13.2 Stage 8 0.031 9.2 Stage 10 0.027 7.9 Stage 12 0.021 4.3 Nitrogen Balance
  • 102. Influent Effluent Flow(gpd) 25,000 --- HRT(hrs) 16 --- BOD(ppm) 650 <225 TSS(ppm) 650 <225 Wal-Mart, Alpharetta, GA 25,000 GPD Pretreatment Facility
  • 103. Influent Effluent Flow(gpd) 85,000 --- HRT(hrs) 24 --- BOD(ppm ) 300 <25 TSS(ppm) 300 <35 NH3(ppm) 40 <1 TN (ppm) --- <15 Villarrin de Campos - Zamora, Spain  Retrofit  Improved performance
  • 104. Villarrin de Campos - Zamora, Spain
  • 105. MSABP Operations Savings Per MGD 1,900 #/day waste sludge generation Capital Cost 20 day storage digester (450,000 gal tank @ $600 yd3 concrete) $200,000 Aerobic digester aeration system $50,000 Aerobic digester blower system $75,000 Belt press, polymer feed system $150,000 Building, HVAC, etc. $100,000 Total $575,000 Annually Mixing power (30 scfm/kcf @ $0.07 / kw-hr) $35,000 Polymer ($12/ ton dry solids) $4,000 WAS disposal (20% conc. @ $37 / wet ton) $65,000 Total $104,000
  • 106. 20 Year Present Worth Savings* $1,567,100 * 7% discount rate
  • 107. Contact Information  Contact: Jack Akin, MS, PE  Cell Phone: 541-474-9434, Ext. 1  Email: bioscapetechnologies@charter.net  emc@emcengineersscientists.com  Jacksonville Office Address: 450 Conestoga Drive, Jacksonville, Oregon, 97530