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During transformation, only one plasmid enters an E. coli cell that is competent to take up DNA.
What could happen if multiple plasmids entered one E. coli cell?
Answer choices:
DNA ligase would not seal phosphodiester bonds.
The E. coli cells would not be resistant to antibiotics.
Colonies would spread out on the plate and touch each other.
There could be a mixture of different plasmids in each colony.
The E. coli cells could not replicate.

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During transformation only one plasmid enters an E coli ce.pdf

  • 1. During transformation, only one plasmid enters an E. coli cell that is competent to take up DNA. What could happen if multiple plasmids entered one E. coli cell? Answer choices: DNA ligase would not seal phosphodiester bonds. The E. coli cells would not be resistant to antibiotics. Colonies would spread out on the plate and touch each other. There could be a mixture of different plasmids in each colony. The E. coli cells could not replicate.