The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.

1. The comet assay is a sensitive, well established technique for quantifying DNA damage in
eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its
principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA
forming the tail of the comet), while intact DNA moves at a slower rate (head of the
comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA
damage.
Automated
Comet assay
CTRL Etoposide 500µM
Study DNA integrity
Example of Etoposide
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Comet slide
Ask for our short report on HepG2 cells exposed to our in
house genotoxicity library !
Cells: - suspension or adherent
- primary cells or cell line
- with or without S9
Compounds: powder or in stock solution
HepG2 cells exposed 3 hours to several concentrations of Etoposide.
Trevigen automated
96 wells comet assay
Requirements
SegmentationSegmentation
DNA DNA
G E N O T O X I C I T Y