SITE DIRECTED MUTAGENESIS.HARIS

SITE DIRECTED MUTAGENESIS ,[object Object]

[object Object],[object Object]

Basic Mechanism of Site-directed mutagenesis + polymerase + primer replication Mutant translation Wild type translation Only one amino acid changed Wild type protein Mutant protein primer (1) (2) (3) (5) (4) (6) Val -> Thr Smith (1993) Juang RH (2004) BCbasics CAG GTC CAG G C C CAG G C C CAG G C C C G G Thr GTC CAG Val

Primer design for site-directed mutagenesis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],5’ 5’ 3’ 3’ * *

TYPES OF SDM ,[object Object],[object Object],[object Object],[object Object]

Cassette Mutagenesis ,[object Object],[object Object],[object Object]

Random Chemical Mutagenesis ,[object Object],[object Object],[object Object],[object Object]

Chemical Target Na bisulphite Nitrous acid Formic acid Hydrazine dC dT dC Du dA dHX Glycosyl bonds break. Pyrimidine ring break.

Applications of SDM ,[object Object],[object Object],[object Object],[object Object],[object Object]

Papers and useful websites ,[object Object],[object Object],[object Object],[object Object],[object Object]

Thank You haris.p mac ernakulam

SITE DIRECTED MUTAGENESIS.HARIS

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