Laboratory safety guidelines

1. Hani A. Ibrahim
M.Sc. in Biological Control
Akre Technical Institute
8 / 11 / 2015

3. Laboratory safety guidelines
1. Make sure the names of the chemicals before
using them.
2. Do not touch any chemicals, do not taste nor
smell them.
3. Make sure that nearby materials non-flammable
When using flame.
4. Be cautious with Bunsen burners or open flames
– keep flammable substances away from flame.

5. 5. Wearing lab coats, goggles and gloves when using the
chemicals.
6. Wash hands at end of each laboratory period with
soaps and water.
Laboratory safety guidelines
7. Washing and cleaning the tools
then put in their place in the
laboratory, clean the bench and your
seat before leaving the laboratory.

6. The required equipments and tools in the
plant diseases laboratories
1- Hood cabinet:
A room with ultraviolet light to sterilize the
inside, used for isolating the microorganism
1- Equipments

7. 2-Dry oven
• work with gas or electric, used for sterilization.
• It can use a temperature between 150-180 °C
for 3-5 hours to sterilize of glass and metal tools
and drying of samples.

8. 3-Hot plate
• Used for drying and heating the solutions which
requires raising the temperature for their
preparation such as culture media of organism.

9. 4-A water bath is a laboratory equipment made
from a container filled with heated water. It is used
to incubate samples in water at a constant
temperature over a long period of time for ex.
culture media
Refrigerator

10. 5-Balance are devices to measure weight of
samples

11. 6-Autoclave is a pressure chamber used to sterilize
equipment and supplies by subjecting them to high
pressure saturated steam at 121 °C (249°F) for
around 15–20 minutes depending on the size of the
load and the contents

12. 7-Distilled water is water that has many of its
impurities removed like mineral and salt through
distillation. Distillation involves boiling the water
and then condensing the steam into a clean
container.

13. 8- Incubator is a device used to grow and maintain microbiological
cultures or cell cultures. The incubator maintains optimal temperature,
humidity and other conditions such as the carbon dioxide (CO2) and
oxygen content of the atmosphere inside. Incubators are essential for a
lot of experimental work in cell biology, microbiology and molecular
biology and are used to culture both bacterial as well as eukaryotic
cells. The most commonly used temperature both for bacteria37 °C
and fungi 25 °C

14. 9-A shaker is a device used in chemistry and
biology laboratories to stir liquids.

15. 2- Glass Wares
1-Conical Flasks
2-Beakers

16. 3-Volumetric Flasks
4-Measuring Cylinders

17. 5-Burettes
6-Test tubes

18. 7-Pipette
8-Petri dishes

19. 9-Slides
10-Cover Slips

20. 1-Inoculation needles
2-Forceps
3-Scissors
3-Non Glass Wares
4- Blade

21. 5-Washing bottles
6-Bunsen flame
7-Filter paper