1. LAB REVIEW PARASITES & BLOOD CELLS

2. Band Neutrophil

3. Red Blood Cells

4. Lymphocyte

5. Eosinophil

6. Platelets

7. Basophil (in horses it is blue)

8. Monocyte

9. Segmented Neutrophil

10. Tapeworm

11. Hookworm

12. Heartworm Microfilaria

13. Roundworm

14. Coccidia

15. Whipworm

16. Scabies Mite

17. Demodex

18. Giardia

19. FLEA

20. Otodectes Cynotis Mite

21. Babesia Canis

22. Sucking Lice…………..Biting Lice

23. Ehrlichia

24. Dermacentor Variabilis….Black Leg Tick

25. Lone Star Tick………American Dog Tick

26. Dirofilaria immitum….dipeteloma reconditum

27. Avian Blood

28. Warble or Cuterebra (fly larvae)

29. Anaplasma Marginale

30. Lung Worm

31. paragonimus kellicotti

32. Lung Fluke

33. Eucoleus boehmi

34. Urine Physical Exam: Smell Color Turbidity Foam

35. Urine Chemical Exam: pH Bilirubin Protein Occult Blood Glucose Ketones Specific gravity Nitrites WBCs

36. Heartworm Test Buffy Coat method (hematocrit test) Modified Knott’s technique Filter membrane technique/Di-fil Blood Smear SNAP: Antigen testing does not detect the actual parasite but the host’s response to the disease

37. Steps For Modified Knotts Mix 1 ml of blood w/ 9ml of 2% formalin in centrifuge Centrifuge tube at 1300 to 1500 rpm for 5 minutes Pour off liquid supernatant, leaving sediment at bottom of tube Add 2-3 drops of stain to sediment. Using pipette, mix sediment w/stain Place drop of this mixture on glass slide. Apply coverslip. Examine for microfilariae using 10X objective. Filter Membrane Mix 1 ml of blood w/9 ml of lysing solution in 15 ml test tube Place filter membrane in filter holder, close tightly, do not pinch seal in threads of holder Connect syringe to filter holder and gently empty through filter holder Fill syringe with water and repeat above step Open holder, use thumb forceps to remove filter membrane Place filter membrane on a clean microscope slide Add one drop of stain, cover with coverslip, examine for microfilariae

38. Stages of a Parasites Life Cycle 1. oocyst 2. Larvae 3. nymph 4. Pupa 5. Adult