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Mark W. Davey,
                     Prof. Wannes Keuelmans
                       Dept. Biosystems,
                          KU Leuven
                      mark.davey@biw.kuleuven.be

http://www.biw.kuleuven.be/biosyst/plantenbiotechniek/Fruitgenetics/LFBB/

                                                                    Madav – FruitBreedomics Feb 2012
Lab Fruit Breeding & Biotechnol




                                  Madav – FruitBreedomics Feb 2012
Research Activities

                  Fruit quality
                     • antioxidants
                     • texture
                     • quality traits (taste,
                       acidity..)



Development                                     Resistance
 •flowering                                       •abiotic stress
 •pollination                                     •biotic stress (Venturia
 •fruit set                                        inaequalis, storage..)


                Tree architecture
                  •production efficiencies,
                  •yelds and yield predication
                  •correlative effects

  HIGH QUALITY, SUSTAINABLE FRUIT PRODUCTION
                                                                 Madav – FruitBreedomics Feb 2012
Research at KU Leuven

Projects:
1. QTL & marker development for fruit quality traits
2. Vitamin C , antioxidant metabolism and (a)biotic stress response
3. Genetic control of tree architecture
4. Proteomics of Malus - Venturia interactions
5. Pear-scab – Venturia pirina
6. Induced resistance and cross-tolerance to post-harvest Botrytis
   infection in Malus
7. Polyploidisation as a means to manipulate crops stress tolerance
8. Carotenoid (vitamin A) metabolism and diversity in Musa

                    FruitBreedomics: WP3

                                                      Madav – FruitBreedomics Feb 2012
NGS - SNP Discovery




RNASeq 14 cultivars:
   flower- and leaf buds
   8 developmental stages,
   7 old cv’s and 7 commercial cultivars
                                           Madav – FruitBreedomics Feb 2012
NGS - SNP Discovery



 reference
  CDS
  cv-1

  cv-2



SNPs cv-1
SNPs cv-2




                                   Madav – FruitBreedomics Feb 2012
Transcript Discovery




                       Madav – FruitBreedomics Feb 2012
APPLE 8K SNP CHIP




• Evaluation of Apple SNP-chip in germplasm
  collection and mapping population
                                          Madav – FruitBreedomics Feb 2012
KU Leuven




Thanks!




                Madav – FruitBreedomics Feb 2012

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FruitBreedomics 1st Annual meeting 20120208 Presentation of new partners KUL

  • 1. Mark W. Davey, Prof. Wannes Keuelmans Dept. Biosystems, KU Leuven mark.davey@biw.kuleuven.be http://www.biw.kuleuven.be/biosyst/plantenbiotechniek/Fruitgenetics/LFBB/ Madav – FruitBreedomics Feb 2012
  • 2. Lab Fruit Breeding & Biotechnol Madav – FruitBreedomics Feb 2012
  • 3. Research Activities Fruit quality • antioxidants • texture • quality traits (taste, acidity..) Development Resistance •flowering •abiotic stress •pollination •biotic stress (Venturia •fruit set inaequalis, storage..) Tree architecture •production efficiencies, •yelds and yield predication •correlative effects HIGH QUALITY, SUSTAINABLE FRUIT PRODUCTION Madav – FruitBreedomics Feb 2012
  • 4. Research at KU Leuven Projects: 1. QTL & marker development for fruit quality traits 2. Vitamin C , antioxidant metabolism and (a)biotic stress response 3. Genetic control of tree architecture 4. Proteomics of Malus - Venturia interactions 5. Pear-scab – Venturia pirina 6. Induced resistance and cross-tolerance to post-harvest Botrytis infection in Malus 7. Polyploidisation as a means to manipulate crops stress tolerance 8. Carotenoid (vitamin A) metabolism and diversity in Musa FruitBreedomics: WP3 Madav – FruitBreedomics Feb 2012
  • 5. NGS - SNP Discovery RNASeq 14 cultivars: flower- and leaf buds 8 developmental stages, 7 old cv’s and 7 commercial cultivars Madav – FruitBreedomics Feb 2012
  • 6. NGS - SNP Discovery reference CDS cv-1 cv-2 SNPs cv-1 SNPs cv-2 Madav – FruitBreedomics Feb 2012
  • 7. Transcript Discovery Madav – FruitBreedomics Feb 2012
  • 8. APPLE 8K SNP CHIP • Evaluation of Apple SNP-chip in germplasm collection and mapping population Madav – FruitBreedomics Feb 2012
  • 9. KU Leuven Thanks! Madav – FruitBreedomics Feb 2012