2. General description of promoters ( structure,
function)
types of promoters based on CG content
Bidirectional promoters
General types of Promoters
3. a promoter is a region of DNA that initiates transcription of a
particular gene.
Promoters are located near the transcription start sites of
genes, on the same strand and upstream on the DNA
(towards the 5' region of the sense strand).
Promoters can be about 100–1000 base pairs long
4.
5. Conserved eukaryotic
promoter elements
Consensus sequence
CAAT box GGCCAATCT
TATA box TATAA
GC box GGGCGG
CAP site TAC
6. • RNA polymerase binding site
• Initiation of transcription
• control by regulatory sequences => control the
expression of genes
7. • - 35 box and – 10 box ( also called Pribnow box) are
consensus sequences
• The two boxes are far appart from a specific
distance, so they are located on the same face of the
double helix.
8. There are two parts:
- The core promoter or basal promoter
- Upstream promoter element ( one or more)
Core promoter is constituted by the TATA box and the transcriptional start
site (TSS)
Initation complexe bind to the core promoter
Upstream elements are responsable of the regulation of the transcription
9. • Two categories of regulatory sequences:
- Activating sequences= Enhancers
- Repressing sequences = Silencers
• In Prokaryotes, regulatory sequences are located around the
promoter
• In Eukaryotes, regulatory sequences are often upstream the
promoter at about 100 bp away, but sometime they are far away
(several thousand nucleotides) or they can also be dowstream
from the promoter
10.
11. CpG = Cytosine followed by a Guanine ( linked together by a phosphate)
CpG island = regions of the DNA which have a high concentration of CpG
Based on CpG content, there are two types of promoters:
- with high CpG content ( called HCG) = 72%
- CpG content characteristic of averall genome = 28%
In HCG, the CpG region is symetric and peaks aroud the core promoter
(specially around the TSS).
12. • Occurs on Cytosine in 5’ position
• inhibits transcription if methylated region is
close to a promoter, which is the case for CpG
islands.
• CpG island associated with HCG are often
hypomthylated =>more expressed . It is often
associted with House-keeping gene.
•Whereas, in promoter with lower CpG, CpG
are often methylated => inhibit the expression.
This often finds in tissue-specific genes.
• With CpG islands around promoter, posibility
to have methylation mediated regulation
13.
14. • Pairs of genes control by same promoter but located on opposite strand
and opposite direction. Their TSS are separated by less than 1,000 bp.
• In general, they are rich in CpG content
• Function of genes represented in bidirectional class are often: DNA repair
genes, chaperone protein, and mitochondrial genes.
• Genes control by bidirectional promoters are often coexpress, but a
minority of bidirectional genes have a mutual exclusive expression.
• No correlation between lenght of promoter and degree of expression
15. Genes of bidirectional promoters shared some element
of the promoter
If there is deletion of TSS of one transcript, the
transcription of the gene on the opposite direction is
increased.
bidirectional promoter acts as an inseparable functional
units which regulate the transcription of both genes.
17. Induce the expression of the downstream-located coding
region in all tissues irrespective of environmental or
developmental factors.
e.g: plant pathogen promoters(CaMV 35S promoter)
18. the activity of these promoters is induced by the presence or
absence of biotic or abiotic factors.
inducible promoters are grouped as:
1. Chemically-regulated promoters
2. Physically-regulated promoters
19. Operate in particular tissues and at certain developmental
stages
may be induced by endogenous and exogenous factors.
e.g: tomato pz7 and pz130 gene promoters (for ovary gene
expression)
20. comprise consensus DNA sequences of common elements of
natural promoter regions.
maize ubiquitin 1 gene (Ubi-1) core promoter.
cytomegalovirus (CMV) promoter.
CAG promoter(cmv,actin,globin)
