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Point estimation and interval estimation ,[object Object],[object Object],[object Object]
Statistical estimation Population Random sample Parameters Statistics Every member of the  population has the  same chance of being selected in the sample estimation
[object Object],Statistical estimation Point estimate Interval estimate ,[object Object],[object Object],[object Object],[object Object],Point estimate is always within the interval estimate
[object Object],[object Object],Interval estimation Confidence interval (CI)
Interval estimation Confidence interval (CI) -3.0  -2.0  -1.0  0.0  1.0  2.0  3.0 34% 34% 14% 14% 2% 2% z -1.96 1.96 -2.58 2.58
Interval estimation Confidence interval (CI), interpretation and example  x= 41.0, SD= 8.7, SEM=0.46,  95% CI (40.0, 42),  99%CI (39.7, 42.1) Age in years 60.0 57.5 55.0 52.5 50.0 47.5 45.0 42.5 40.0 37.5 35.0 32.5 30.0 27.5 25.0 22.5 Frequency 50 40 30 20 10 0
Testing of hypotheses ,[object Object],[object Object],[object Object],[object Object]
Statistical inference. Role of chance. Formulate  hypotheses Collect data to test hypotheses
Statistical inference. Role of chance. Formulate  hypotheses Collect data to test hypotheses Accept hypothesis Reject hypothesis C H A N C E Random error (chance) can be controlled by statistical significance or by confidence interval Systematic error
Testing of hypotheses Significance test Subjects:  random sample of 352 nurses from HUS surgical    hospitals Mean age of the nurses (based on sample) : 41.0 Another random sample gave mean value:  42.0. Question:   Is it possible that the “true” age of nurses from HUS surgical hospitals was 41 years and observed mean ages differed just because of sampling error? Answer  can be given based on  Significance Testing .
Testing of hypotheses ,[object Object],[object Object],[object Object],[object Object]
Testing of hypotheses Example ,[object Object],[object Object],Research question:   Does the lactation nurse have an effect on attitudes towards breast feeding ? H A  :   The lactation nurse  has an effect  on attitudes towards breast feeding. H 0  :   The lactation nurse  has no effect  on attitudes towards breast feeding.
Testing of hypotheses Definition of p-value. 95% 2.5% 2.5% If our observed age value lies outside the green lines, the probability of getting a value as extreme as this  if the null hypothesis is true  is < 5%
Testing of hypotheses Definition of p-value. p-value  = probability of observing a value more extreme that actual value observed, if the null hypothesis is true The smaller the p-value, the more unlikely the null hypothesis seems an explanation for the data Interpretation for the example If results falls  outside  green lines,  p<0.05 ,  if it falls  inside  green lines,  p>0.05
Testing of hypotheses   Type I and Type II Errors    -   level of significance 1-    -   power of the test No study is perfect,  there is always the chance for error
Testing of hypotheses Type I and Type II Errors ,[object Object],α  =0.05 there is only 5 chance in 100 that the result termed &quot;significant&quot; could occur by chance alone it will be more difficult to find a significant result the power of the test will be decreased  the risk of a Type II error will be increased
Testing of hypotheses Type I and Type II Errors ,[object Object],it will increase the chance of a Type I error To which type of error you are willing to risk  ?
Testing of hypotheses Type I and Type II Errors. Example ,[object Object],[object Object],[object Object],[object Object],To which type of error you are willing to risk  ?
Testing of hypotheses   Type I and Type II Errors. Example. treated but not harmed  by the treatment irreparable damage would be done Decision:   to avoid Type error II, have high level of  significance
Testing of hypotheses Confidence interval and significance test A value for null hypothesis within the 95% CI A value for null hypothesis outside of 95% CI  p-value > 0.05 p-value < 0.05 Null hypothesis is accepted Null hypothesis is rejected
Parametric and nonparametric tests of significance ,[object Object],[object Object],[object Object],[object Object]
Parametric and nonparametric tests of significance ,[object Object],[object Object],[object Object]
Parametric and nonparametric tests of significance
Some concepts related to the statistical methods. ,[object Object],[object Object],[object Object],[object Object]
Some concepts related to the statistical methods. Sample size number of cases, on which data have been obtained Which of the basic characteristics of a distribution are more sensitive to the sample size ? central tendency (mean, median, mode) variability (standard deviation, range, IQR) skewness kurtosis mean standard deviation skewness kurtosis
Some concepts related to the statistical methods. Degrees of freedom the number of scores, items, or other units in the  data set, which are free to vary One- and two tailed tests one-tailed test of significance used for directional hypothesis two-tailed tests in all other situations
Selected nonparametric tests  Chi-Square goodness of fit test. ,[object Object],[object Object],[object Object],[object Object],[object Object]
Selected nonparametric tests  Chi-Square goodness of fit test. Example ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],expected observed Does observed frequencies differ from expected ?
Selected nonparametric tests  Chi-Square goodness of fit test. Example ,[object Object],[object Object],[object Object], 2 = 14.2, df=3  (4-1) 0.0005 < p < 0.05  Null hypothesis is rejected at 5% level  2  > 3.841  p < 0.05  2  > 6.635  p < 0.01  2  > 10.83  p < 0.001
Selected nonparametric tests  Chi-Square test.  ,[object Object],Expected frequencies can be calculated: then df = ( f r -1 ) ( f c -1 )
Selected nonparametric tests  Chi-Square test. Example ,[object Object],Sample:  people have similar results on initial testing Response:  whether or not a cardiac catheterization was recommended Independent:  sex of the patient
Selected nonparametric tests  Chi-Square test. Example ,[object Object]
Selected nonparametric tests  Chi-Square test. Example ,[object Object]
Selected nonparametric tests  Chi-Square test. Example ,[object Object], 2 = 2.52, df=1  (2-1) (2-1) p > 0.05  Null hypothesis is accepted at 5% level Conclusion:  Recommendation for cardiac catheterization is not related to the sex of the patient
Selected nonparametric tests  Chi-Square test. Underlying assumptions. ,[object Object],[object Object],[object Object],[object Object],Cannot be used to analyze differences in scores or their means Expected frequencies should not be less than 5 No subjects can be count more than once Categories should be defined prior to data collection and analysis
Selected nonparametric tests  Fisher’s exact test. McNemar test. ,[object Object],[object Object]
Parametric and nonparametric tests of significance
Selected nonparametric tests  Ordinal data independent groups. ,[object Object],Kruskal-Wallis H:  used to compare two or more groups
Selected nonparametric tests  Ordinal data independent groups. Mann-Whitney test The observations from both groups are combined and ranked, with the average rank assigned in the case of ties.  Null hypothesis  :  Two sampled  populations are    equivalent in location If the populations are identical in location, the ranks should be randomly mixed between the two samples
Selected nonparametric tests  Ordinal data independent groups. Kruskal-Wallis test The observations from all groups are combined and ranked, with the average rank assigned in the case of ties.  Null hypothesis  :  k sampled  populations are    equivalent in location If the populations are identical in location, the ranks should be randomly mixed between the k samples  k- groups comparison, k    2
Selected nonparametric tests  Ordinal data related groups.  ,[object Object],[object Object],Friedman matched samples:   used to compare two or more related groups
Selected nonparametric tests  Ordinal data 2 related groups Wilcoxon signed rank test Takes into account information about the  magnitude of differences within pairs  and gives more weight to pairs that show large differences than to pairs that show small differences.  Null hypothesis  :  Two variables have the same    distribution Based on  the ranks of the absolute values of the differences  between the two variables. Two related variables. No assumptions about the shape of distributions of the variables.
Parametric and nonparametric tests of significance
Selected parametric tests  One group t-test. Example Comparison of sample mean with a population mean Question:  Whether the studed group have a significantly lower body weight than the general population? It is known   that the weight of young adult male has a mean value of 70.0 kg with a standard deviation of 4.0 kg.  Thus the population mean, µ= 70.0 and population standard deviation, σ= 4.0.  Data from random sample of 28 males of similar ages but with specific enzyme defect:   mean body weight of 67.0 kg and the sample standard deviation of 4.2 kg.
Selected parametric tests  One group t-test. Example Null hypothesis:  T here is no difference between sample mean and population mean . population mean, µ= 70.0  population standard deviation,  σ= 4.0.   sample size = 28 sample mean,   x = 67.0  sample standard deviation,  s= 4.0.   t - statistic = 0.15,  p >0.05 Null hypothesis is accepted at 5% level
Selected parametric tests  Two unrelated group,  t-test. Example Comparison of means from  two unrelated groups Study of the effects of anticonvulsant therapy on bone disease in the elderly.  Study design: Samples:   group of treated patients ( n=55 )  group of untreated patients ( n=47 ) Outcome measure:   serum calcium concentration Research question:  Whether the groups statistically  significantly differ in mean serum consentration? Test of significance:  Pooled t-test
Selected parametric tests  Two unrelated group,  t-test. Example Comparison of means from  two unrelated groups Study of the effects of anticonvulsant therapy on bone disease in the elderly.  Study design: Samples:   group of treated patients ( n=20 )  group of untreated patients ( n=27 ) Outcome measure:   serum calcium concentration Research question:  Whether the groups statistically  significantly differ in mean serum consentration? Test of significance:  Separate  t-test
Selected parametric tests  Two related group,  paired t-test. Example Comparison of means from  two related variabless Study of the effects of anticonvulsant therapy on bone disease in the elderly.  Study design: Sample:   group of treated patients (n=40)  Outcome measure:   serum calcium concentration  before and after operation Research question:  Whether the mean serum  consentration statistically  significantly differ before and after operation? Test of significance:  paired  t-test
Selected parametric tests  k  unrelated group,  one -way ANOVA test. Example Comparison of means from  k unrelated groups Study of the effects of two different drugs (A and B) on weight reduction.  Study design: Samples:  group of patients treated with drug A  (n=32)  group of patientstreated with drug B  (n=35)  control group  (n=40) Outcome measure:  weight reduction Research question:  Whether the groups statistically  significantly differ in mean weight reduction? Test of significance:  one -way ANOVA test
Selected parametric tests  k  unrelated group,  one -way ANOVA test. Example T he group means compared with the overall mean of the sample Visual examination of the individual group means may yield no clear answer about which of the means are different Additionally post-hoc tests can be used (Scheffe or Bonferroni)
Selected parametric tests  k  related group,  two -way ANOVA test. Example Comparison of means for k related variables Study of the effects of drugs A  on weight reduction.  Study design: Samples:  group of patients treated with drug A  ( n=35 )  control group  ( n=40 ) Outcome measure:  weight in Time 1 (before using  drug) and Time 2 (after using drug)
Selected parametric tests  k  related group,  two -way ANOVA test. Example   Research questions:  ,[object Object],[object Object],Test of significance:  ANOVA with repeated measurement test ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Time effect Group difference Drug effect
Selected parametric tests  Underlying assumptions. ,[object Object],[object Object],[object Object],[object Object],Cannot be used to analyze frequency  Sample size big enough to avoid skweness No subjects can be belong to more than one group Equality of group variances
Parametric and nonparametric tests of significance
Att rapportera resultat i text ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Beskrivning av samplet ,[object Object]
Faktoranalysen ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Dtaanlysmetoder ,[object Object],[object Object]

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Hypo

  • 1.
  • 2. Statistical estimation Population Random sample Parameters Statistics Every member of the population has the same chance of being selected in the sample estimation
  • 3.
  • 4.
  • 5. Interval estimation Confidence interval (CI) -3.0 -2.0 -1.0 0.0 1.0 2.0 3.0 34% 34% 14% 14% 2% 2% z -1.96 1.96 -2.58 2.58
  • 6. Interval estimation Confidence interval (CI), interpretation and example  x= 41.0, SD= 8.7, SEM=0.46, 95% CI (40.0, 42), 99%CI (39.7, 42.1) Age in years 60.0 57.5 55.0 52.5 50.0 47.5 45.0 42.5 40.0 37.5 35.0 32.5 30.0 27.5 25.0 22.5 Frequency 50 40 30 20 10 0
  • 7.
  • 8. Statistical inference. Role of chance. Formulate hypotheses Collect data to test hypotheses
  • 9. Statistical inference. Role of chance. Formulate hypotheses Collect data to test hypotheses Accept hypothesis Reject hypothesis C H A N C E Random error (chance) can be controlled by statistical significance or by confidence interval Systematic error
  • 10. Testing of hypotheses Significance test Subjects: random sample of 352 nurses from HUS surgical hospitals Mean age of the nurses (based on sample) : 41.0 Another random sample gave mean value: 42.0. Question: Is it possible that the “true” age of nurses from HUS surgical hospitals was 41 years and observed mean ages differed just because of sampling error? Answer can be given based on Significance Testing .
  • 11.
  • 12.
  • 13. Testing of hypotheses Definition of p-value. 95% 2.5% 2.5% If our observed age value lies outside the green lines, the probability of getting a value as extreme as this if the null hypothesis is true is < 5%
  • 14. Testing of hypotheses Definition of p-value. p-value = probability of observing a value more extreme that actual value observed, if the null hypothesis is true The smaller the p-value, the more unlikely the null hypothesis seems an explanation for the data Interpretation for the example If results falls outside green lines, p<0.05 , if it falls inside green lines, p>0.05
  • 15. Testing of hypotheses Type I and Type II Errors  - level of significance 1-  - power of the test No study is perfect, there is always the chance for error
  • 16.
  • 17.
  • 18.
  • 19. Testing of hypotheses Type I and Type II Errors. Example. treated but not harmed by the treatment irreparable damage would be done Decision: to avoid Type error II, have high level of significance
  • 20. Testing of hypotheses Confidence interval and significance test A value for null hypothesis within the 95% CI A value for null hypothesis outside of 95% CI p-value > 0.05 p-value < 0.05 Null hypothesis is accepted Null hypothesis is rejected
  • 21.
  • 22.
  • 23. Parametric and nonparametric tests of significance
  • 24.
  • 25. Some concepts related to the statistical methods. Sample size number of cases, on which data have been obtained Which of the basic characteristics of a distribution are more sensitive to the sample size ? central tendency (mean, median, mode) variability (standard deviation, range, IQR) skewness kurtosis mean standard deviation skewness kurtosis
  • 26. Some concepts related to the statistical methods. Degrees of freedom the number of scores, items, or other units in the data set, which are free to vary One- and two tailed tests one-tailed test of significance used for directional hypothesis two-tailed tests in all other situations
  • 27.
  • 28.
  • 29.
  • 30.
  • 31.
  • 32.
  • 33.
  • 34.
  • 35.
  • 36.
  • 37. Parametric and nonparametric tests of significance
  • 38.
  • 39. Selected nonparametric tests Ordinal data independent groups. Mann-Whitney test The observations from both groups are combined and ranked, with the average rank assigned in the case of ties. Null hypothesis : Two sampled populations are equivalent in location If the populations are identical in location, the ranks should be randomly mixed between the two samples
  • 40. Selected nonparametric tests Ordinal data independent groups. Kruskal-Wallis test The observations from all groups are combined and ranked, with the average rank assigned in the case of ties. Null hypothesis : k sampled populations are equivalent in location If the populations are identical in location, the ranks should be randomly mixed between the k samples k- groups comparison, k  2
  • 41.
  • 42. Selected nonparametric tests Ordinal data 2 related groups Wilcoxon signed rank test Takes into account information about the magnitude of differences within pairs and gives more weight to pairs that show large differences than to pairs that show small differences. Null hypothesis : Two variables have the same distribution Based on the ranks of the absolute values of the differences between the two variables. Two related variables. No assumptions about the shape of distributions of the variables.
  • 43. Parametric and nonparametric tests of significance
  • 44. Selected parametric tests One group t-test. Example Comparison of sample mean with a population mean Question: Whether the studed group have a significantly lower body weight than the general population? It is known that the weight of young adult male has a mean value of 70.0 kg with a standard deviation of 4.0 kg. Thus the population mean, µ= 70.0 and population standard deviation, σ= 4.0. Data from random sample of 28 males of similar ages but with specific enzyme defect: mean body weight of 67.0 kg and the sample standard deviation of 4.2 kg.
  • 45. Selected parametric tests One group t-test. Example Null hypothesis: T here is no difference between sample mean and population mean . population mean, µ= 70.0 population standard deviation, σ= 4.0. sample size = 28 sample mean,  x = 67.0 sample standard deviation, s= 4.0. t - statistic = 0.15, p >0.05 Null hypothesis is accepted at 5% level
  • 46. Selected parametric tests Two unrelated group, t-test. Example Comparison of means from two unrelated groups Study of the effects of anticonvulsant therapy on bone disease in the elderly. Study design: Samples: group of treated patients ( n=55 ) group of untreated patients ( n=47 ) Outcome measure: serum calcium concentration Research question: Whether the groups statistically significantly differ in mean serum consentration? Test of significance: Pooled t-test
  • 47. Selected parametric tests Two unrelated group, t-test. Example Comparison of means from two unrelated groups Study of the effects of anticonvulsant therapy on bone disease in the elderly. Study design: Samples: group of treated patients ( n=20 ) group of untreated patients ( n=27 ) Outcome measure: serum calcium concentration Research question: Whether the groups statistically significantly differ in mean serum consentration? Test of significance: Separate t-test
  • 48. Selected parametric tests Two related group, paired t-test. Example Comparison of means from two related variabless Study of the effects of anticonvulsant therapy on bone disease in the elderly. Study design: Sample: group of treated patients (n=40) Outcome measure: serum calcium concentration before and after operation Research question: Whether the mean serum consentration statistically significantly differ before and after operation? Test of significance: paired t-test
  • 49. Selected parametric tests k unrelated group, one -way ANOVA test. Example Comparison of means from k unrelated groups Study of the effects of two different drugs (A and B) on weight reduction. Study design: Samples: group of patients treated with drug A (n=32) group of patientstreated with drug B (n=35) control group (n=40) Outcome measure: weight reduction Research question: Whether the groups statistically significantly differ in mean weight reduction? Test of significance: one -way ANOVA test
  • 50. Selected parametric tests k unrelated group, one -way ANOVA test. Example T he group means compared with the overall mean of the sample Visual examination of the individual group means may yield no clear answer about which of the means are different Additionally post-hoc tests can be used (Scheffe or Bonferroni)
  • 51. Selected parametric tests k related group, two -way ANOVA test. Example Comparison of means for k related variables Study of the effects of drugs A on weight reduction. Study design: Samples: group of patients treated with drug A ( n=35 ) control group ( n=40 ) Outcome measure: weight in Time 1 (before using drug) and Time 2 (after using drug)
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  • 54. Parametric and nonparametric tests of significance
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