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SAFETY AND FIRST AID IN
THE LABORATORY
Definition
 First aid is the assistance given to any
person suffering a sudden illness or
injury with care provided to preserve
life, prevent the condition from
worsening, and/or promote recovery.
Injuries caused by broken glass:
 Wash the wound immediately to remove any
glass pieces.
 Apply mercurochrome or acriflavine ointment
to the wound.
 Cover with gauze and adhesive tape
Acid/Alkali splashes on the skin:
 Wash thoroughly; bath the affected skin with
cotton wool soaked in 5% aqueous sodium.
 carbonate if acid and 5% acetic acid or
undiluted vinegar, if alkali.
Acid/Alkali splashes in the eye:
 Water spray from a wash bottle or rubber bulb
into the medial corner of the eye .
 Put 4 drops of 2% Aqueous Sodium
bicarbonate into the eye, if acid, and saturated
solution of boric acid, if alkali.
Swallowing acid:
 Make the patient drink some 5% soap solution
immediately. Make him gargle with the soap
solution.
 Give him 3 or 4 glasses of ordinary water. If the
lips and tongue are burned by the acid, rinse
thoroughly with water. Bathe with 2% aqueous
sodium bicarbonate.
Swallowing alkalies:
 Make the patient drink 5% solution of acetic
acid or lemon juice or dilute vinegar.
 Make him gargle with the same acid solution,
Give him 3 or 4 glasses of ordinary water.
 If the lips and tongue are burned by the alkali,
rinse thoroughly with water; bathe with 5%
acetic acid.
Poisoning
 Send for a physician or qualified nurse.
 specifying the toxic substance involved.
 Place the victim in the open air while waiting for
the physician.
Burns caused by heat:
Sever burns
 If the victim is on fire, roll him in a blanket or
overall to smoothen the flames.
 Inform the physician. Lay the victim on the
ground.
 Do not remove his clothing. Cover him if he is
cold. Do not apply any treatment to the burns.
(This must be left to the physician)
Minor burns
 Plunge the affected part into cold water or ice-
water to soothe the pain.
 Apply Mercurochrome or Acriflavine ointment
to the burn.
 Apply dry gauze dressing loosely. If the burn
becomes infected or does not heal, refer the
patient to a physician(Never tear off the blisters
that form over the burns).
Electrical hazard and its management
 Electrical equipment should not be handled with
wet hands, nor should electrical equipment be
used after liquid has been spilled on it.
 The equipment must be turned off immediately
and dried thoroughly.
Bodily damage by electric shock:
 The symptoms are fainting and asphyxia.
 Before doing anything else, put off the main
switch. Send for a physician.
 Begin giving mouth to mouth respiration
immediately.
Fire hazard and its management
 Fire in the laboratory may occur due to spirit
lamps, electrical appliances or other all
laboratories should have a fire extinguisher (1, 2,
4), and easy access to safety showers and fire
blankets.
 For putting off the flames from the
inflammable liquids, smoothen the fire by
throwing sand over it.
Biohazard
 Can be infectious agents themselves or items
(solutions, specimens or objects) contaminated
with anything that can cause disease in humans
regardless of its source.
 It is essential to prevent the exposure of
laboratory workers to hepatitis B virus (HBV)
and the human immunodeficiency virus (HIV).
Contaminated by infectious material
 In case of wounds caused by broken
glassware containing stools, pus, etc.,
 wash the wound immediately with
antiseptic lotion.
 Check whether the cut is bleeding. If not,
squeeze hard to make it bleed for several
minutes.
 If infected material is accidentally sucked into
the mouth spit it out immediately. Use a
disinfectant (e.g. Diluted Dettol) for mouth
washing.
 If the infected material has been swallowed
accidentally, forced vomiting is to be done.
The first aid equipment
 Emergency drench showers.
 Eyewash stations.
 Emergency showers.
 Oxygen cylinders.
 Defibrillator.
First aid box
 low grade disinfectant
 adhesive bandages
 pain medication
 gauze
 gloves
 Scissors
Conclusion
Safety awareness should become habit
and a way of life in the laboratory.

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First aids lecture

  • 1. SAFETY AND FIRST AID IN THE LABORATORY
  • 2. Definition  First aid is the assistance given to any person suffering a sudden illness or injury with care provided to preserve life, prevent the condition from worsening, and/or promote recovery.
  • 3. Injuries caused by broken glass:  Wash the wound immediately to remove any glass pieces.  Apply mercurochrome or acriflavine ointment to the wound.  Cover with gauze and adhesive tape
  • 4. Acid/Alkali splashes on the skin:  Wash thoroughly; bath the affected skin with cotton wool soaked in 5% aqueous sodium.  carbonate if acid and 5% acetic acid or undiluted vinegar, if alkali.
  • 5. Acid/Alkali splashes in the eye:  Water spray from a wash bottle or rubber bulb into the medial corner of the eye .  Put 4 drops of 2% Aqueous Sodium bicarbonate into the eye, if acid, and saturated solution of boric acid, if alkali.
  • 6. Swallowing acid:  Make the patient drink some 5% soap solution immediately. Make him gargle with the soap solution.  Give him 3 or 4 glasses of ordinary water. If the lips and tongue are burned by the acid, rinse thoroughly with water. Bathe with 2% aqueous sodium bicarbonate.
  • 7.
  • 8. Swallowing alkalies:  Make the patient drink 5% solution of acetic acid or lemon juice or dilute vinegar.  Make him gargle with the same acid solution, Give him 3 or 4 glasses of ordinary water.  If the lips and tongue are burned by the alkali, rinse thoroughly with water; bathe with 5% acetic acid.
  • 9. Poisoning  Send for a physician or qualified nurse.  specifying the toxic substance involved.  Place the victim in the open air while waiting for the physician.
  • 11. Sever burns  If the victim is on fire, roll him in a blanket or overall to smoothen the flames.  Inform the physician. Lay the victim on the ground.  Do not remove his clothing. Cover him if he is cold. Do not apply any treatment to the burns. (This must be left to the physician)
  • 12. Minor burns  Plunge the affected part into cold water or ice- water to soothe the pain.  Apply Mercurochrome or Acriflavine ointment to the burn.  Apply dry gauze dressing loosely. If the burn becomes infected or does not heal, refer the patient to a physician(Never tear off the blisters that form over the burns).
  • 13. Electrical hazard and its management  Electrical equipment should not be handled with wet hands, nor should electrical equipment be used after liquid has been spilled on it.  The equipment must be turned off immediately and dried thoroughly.
  • 14. Bodily damage by electric shock:  The symptoms are fainting and asphyxia.  Before doing anything else, put off the main switch. Send for a physician.  Begin giving mouth to mouth respiration immediately.
  • 15. Fire hazard and its management  Fire in the laboratory may occur due to spirit lamps, electrical appliances or other all laboratories should have a fire extinguisher (1, 2, 4), and easy access to safety showers and fire blankets.  For putting off the flames from the inflammable liquids, smoothen the fire by throwing sand over it.
  • 16. Biohazard  Can be infectious agents themselves or items (solutions, specimens or objects) contaminated with anything that can cause disease in humans regardless of its source.  It is essential to prevent the exposure of laboratory workers to hepatitis B virus (HBV) and the human immunodeficiency virus (HIV).
  • 17. Contaminated by infectious material  In case of wounds caused by broken glassware containing stools, pus, etc.,  wash the wound immediately with antiseptic lotion.  Check whether the cut is bleeding. If not, squeeze hard to make it bleed for several minutes.
  • 18.  If infected material is accidentally sucked into the mouth spit it out immediately. Use a disinfectant (e.g. Diluted Dettol) for mouth washing.  If the infected material has been swallowed accidentally, forced vomiting is to be done.
  • 19. The first aid equipment  Emergency drench showers.  Eyewash stations.  Emergency showers.  Oxygen cylinders.  Defibrillator.
  • 20. First aid box  low grade disinfectant  adhesive bandages  pain medication  gauze  gloves  Scissors
  • 21. Conclusion Safety awareness should become habit and a way of life in the laboratory.

Notas do Editor

  1. Gargle: غرعرة
  2. Acriflavine is a topical antiseptic. It has the form of an orange or brown powder. It may be harmful in the eyes or if inhaled. It is a dye and it stains the skin and may irritate. Commercial preparations are often mixtures with proflavine. It is known by a variety of commercial names. Acriflavine was developed in 1912 by Paul Ehrlich, a German medical researcher, and was used during the First World War against sleeping sickness. It is derived from acridine. The hydrochloride form is more irritating than the neutral form. Acriflavine is also used as treatment for external fungal infections of aquarium fish.
  3. Asphyxia or asphyxiation (from Ancient Greek α- "without" and σφύξις sphyxis, "heartbeat") is a condition of severely deficient supply of oxygen to the body that arises from abnormal breathing. An example of asphyxia is choking. Asphyxia causes generalized hypoxia, which affects primarily the tissues and organs. There are many circumstances that can induce asphyxia, all of which are characterized by an inability of an individual to acquire sufficient oxygen through breathing for an extended period of time. Asphyxia can cause coma or death. Fainting:unconsciousness