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Integrating Detection of Multiple Pathogens in Food

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Lawrence Goodridge Department of Animal Sciences Colorado State University [email_address] Integrating Detection of Multiple Pathogens in Food
Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Objective ,[object Object]
Biochemical Assays Previously Developed Salmonella Listeria monocytogenes Escherichia coli
Disadvantages of the Biochemical Assays ,[object Object],[object Object],[object Object]
Paper-Based Testing Devices ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Lu et al. 2009
Assay Fabrication with Wax ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Paper Assay for Bacteria
Enzymes and their Substrates
L. monocytogenes Assay: Characterization ,[object Object],[object Object],[object Object],[object Object]
L. monocytogenes Assay ,[object Object],[object Object],[object Object],[object Object],Control 2 hr 3 hr 3.5 hr 6 hr 5 hr 4.5 hr 4 hr
Salmonella assay: pH Studies ,[object Object],[object Object],[object Object],[object Object]
Salmonella Assay ,[object Object],[object Object],[object Object],[object Object],[object Object],6.0 6.5 7.0 7.5 8.0 9.0 control
E. coli Assay: pH Studies ,[object Object],[object Object],[object Object]
E. coli Assay: Limit of detection ,[object Object],[object Object],Varying β -galactosidase (µg/mL) control 0.03 0.06 0.13 0.4 0.33 0.26 0.2
E. coli Assay ,[object Object],[object Object],Using 10 mL of growth media Using 1 mL of growth media for enrichment of E. coli (t = 4.5 hr) 7 mm
Putting it all together: Detection of all the Bacteria ,[object Object],[object Object],[object Object],[object Object],E. coli Salmonella spp. control L. monocytogenes
Future Work ,[object Object],[object Object],[object Object],[object Object],[object Object]
Thank you!

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