The expert consultation on the use of crop wild relatives for pre-breeding in potato was a workshop organized by the Global Crop Diversity Trust in collaboration with CIP and took place from the 22nd – 24th of February 2012.

1. Arturo Brenes Angulo
Centro de Investigaciones Agronómicas
Universidad de Costa Rica
CIP- Global Crop Diversity Trust; February 22-24, 2012, Lima, Perú

2. Solanum laxissimum S. berthaultii

3. Costa Rica
51 100 Km2 (0.03% of the planet’s
surface)
≈ 4,2 Millions Habitants
4% of the total species estimated
worldwide (more than 500 000
species )

5. The potato and its wild relatives;
section Tuberarium of the genus
Solanum.
by Donovan Stewart Correll 1962

7. According to Correll (1962), in Costa Rica existe 8 potato wild
relatives
- S. juglandifolium y S. oxycarpum of the subsectión Hyperbasarthrum
- S. canense, S. caripense, S. fraxinifolium, S. muricatum (introduced),
S. suaveolens y S. taeniotrichum of the subsección Basarthrum

9. Collecting, conservation ,characterization and use of the potato wild relatives
from Costa Rica

15. Solanum woodsonii

16. Solanum longiconicum

17. Solanum taenotrichium

18. Solanum canense

19. Solanum caripense

20. Solanum fraxinifolium

21. Wild Potato Relatives collected in Costa Rica
Species No of
accessions
Solanum canense Ruydb.: 2
Solanum caripense Humb.&Bonpl.Ex.Dun.: 10
Solanum fraxinifolium Dunal in DC: 7
Solanum longiconicum Bitter 12
Solanum taenotrichum Corr.: 3
Solanum woodsonii Corr.: 11
Total 45

26. Leafminer (Liriomyza spp.)

29. Source: www.ecohuellas.files.wordpress.com/.../sequia1.jpg

36. Phenotyping for Drought Stress

38. Chromosome Chromosome
Number PCR Marker Resistance to Number PCR Marker Resistance to
I CP108 E. carotovora ssp. atroseptica VIII ND Rpi-blb1, P.infestans
II GP23 E. carotovora ssp. atroseptica VIII ND Osm8,
III GP1 (a) P.infestans VI ND Rpi-blb2, P.infestans
III GP25 SSCP/TaqI (CAPS) Taq1 PVY, P.infestans IV ND Rpi-blb3, P.infestans
III Ssp8 Gro1.4, G. rostochiensis IX GP94 (SSCP-Hinf I/Dpn I?
Rpi-phu1, P.infestans
III GP276 P.infestans IX Prp1 Prp1, PRP, P.infestans
IV Stm3016 Gpa4, G. pallida, P. infestans IX InvGF-4d Tuber starch
IX Gp6 QTL for G. pallida IX InvGF-4b Chip color
V BA47f2 P.infestans XI GP250 R3, R6, R7, P. infestans
V CM1 H1, G. rostochiensis XI NL25 (SSCP) Sen1, Sinchrytium endobioticum
V CosA P.infestans XI NL27 (3 and 5, Borman et al, 2003)
PLRV.1, PLRV
V CP113 HI, G.rostochiensis XI NL27 SCAR (Marzewski et al, 2001)
PLRV.1, PLRV
V R1general P.infestans XI RYSC3 (SCAR) Ryadg, PVY
V R1specific P.infestans XI CP58 Ryadg, Rysto, PVY, Sen1
Ssp72 GP125 (CAPS)
Taq1
V G.pallida XI PVY,Sen1, P.infestans,
V TG69 Gro VI , G.rostochiensis XI TG523 Rysto,Na adg,RMc1,
GP21 R1, Rx2, NB, Gpa,GPa5, Grp1,P. infestans, PVX,G. Ssp75
V pallida, G. rostochiensis XI Gro1.3, G.rostochiensis
GP179 R1,GPa5, Grp1,P. infestans, PVX,G. pallida,G. TG30
V rostochiensis XI Gro1.3, G.rostochiensis
GP76 GP185 R3, R6, R7, P. infestans ,
VI P. infestans XI E.carotovora ssp. atroseptica
VI GP79 P. infestans XI Osm11,
VI St3.3.13 E. carotovora ssp. atroseptica XI TG105(a) R3 P.infestans
VII CP56 Gro 1, G. rostochiensis XII CP60 (CAPs) Rx1, PVX, P. infestans
VII gro1-11p41 Gro 1, G. rostochiensis XII GP122 PVY
gro1-2p40 (ASO) Rx1, PVX, P. infestans, GPa2,
VII Gro 1, G. rostochiensis XII GP34 (ASO) G.pallida
VII Gro1-4 Gro1, G.pallida X TG63 Rber, P. infestans
VII GP219 E. carotovora ssp. atroseptica XII PR-5 PRP
VIII RC5t7 Osm8, PRP V CT51 (CAPS) H1, G. rostochiensis
VIII SC811 Ns, PVS V 239E4left (CAPS) H1, G. rostochiensis
VIII Osm P.infestans VIII Rpi-blb1 , P.infestans
VIII CP53 Rblc P.infestans VI Rpi-blb2 , P.infestans
IV Th21 (SCAR) Rpi-blb3 , P.infestans

39. Figura 1. Amplificación de fragmentos en especies silvestres de papa de Costa Rica con imprimadores específicos para genes ligados
con resistencia a G. rostochiensis en papa cultivada. M= 100 bp DNA Ladder Plus (Fermentas), C= variedad de papa control P6-210,
17-62 = diferentes accesiones de papa silvestre de Costa Rica.

40. Marcador 239E4left. M: Marcador de peso molecular 100pb. Muestras 1-16 accesiones de papa silvestre, 1: 146, 2:160 3:
134, 4: 147, 5: 130, 6:157, 7:121, 8:127, 9:135, 10: 148, 11:118, 12:150, 13:151, 14:152.

41. Marcador GP179. M: Marcador de peso molecular 100pb. Muestras 1-16 accesiones de papa silvestre, 1: 146, 2:160 3: 134, 4:
147, 5: 130, 6:157, 7:121, 8:127, 9:135, 10: 148, 11:118, 12:150, 13:151, 14:152.
El marcador GP179 (550pb) se ha relacionado con el QTL GpaV (de resistencia a G. pallida) y con el QTL Grp1 de amplio
espectro para G. pallida y G. rostochiensis (Meksem et al.1995).

43. Thanks for your attention