Nematode Testing at Midwest Laboratories. Midwest Laboratories uses a certified nematologist with respect to its nematode analysis. In the fall, many growers try to combat nematode issues by having their soil analyzed for nematodes.

1. Cyst Nematode
Analysis
13611 B Street | Omaha, NE 68144 | (402) 334-7770 | Fax (402) 334-9121
REV 05/14/15

2. Sugar Beet Cyst Nematode
The sugar beet nematode, H. schachtii, is a major parasite of sugar beets, causing
major reductions in yields throughout the midwest. It was first observed in the
United States as early as 1895. Today, the sugar beet nematode is present in
nearly half the states in the U.S. and in 39 other beet growing countries.
Soybean Cyst Nematode
The soybean cyst nematode, H. glycines, is the most important pest of soybeans in
the United States. It was first found in the U.S. in North Carolina around 1954. It
was probably introduced in soil brought over from the Orient in the late 1800’s for
the purpose of obtaining bacteria to nodulate soybean roots. The soybean cyst
nematode now occurs in all major soybean production areas worldwide.
Approximate Distribution of Soybean Cyst Nematode
Heterodera Glycines across the United States
*Source: www.pioneer.com

3. Fee Schedule for Nematode Assays
Plant Parasitic Nematode Identification (Ag Soil) $58.00
Plant Parasitic Nematode Identification (Compost) $68.00
Root Tissue Exam (Root Knot Only) $20.00
Cyst Nematode Only (Egg Count) (Ag Soil) $28.00
Cyst Nematode Only (Egg Count) (Compost) $38.00
Advantages
• Competitive Rates
• Approximately 3-5 working-day turnaround (7-10 business days
for live plant parasitic nematode)
Special rushes can normally be accommodated, please
inquire about our rush surcharges
• Online Reporting
• Latest Equipment & Technique
• Meets USDA/APHIS 526 Requirement for Interstate Movement
of Live Pests
• Meets USDA/APHIS 526 Requirements for Importation of Live
Pests, Noxious Weeds, and Soil. (International Shipment)
• Meets State of Nebraska Plant Protection Regulations
• Consulting available
Visit our website for a complete listing of our fee schedule and online
reporting options at www.midwestlabs.com.
Cyst Nematode Soil Sampling

4. Procedures for collecting samples:
1. Use a soil probe or spade to collect soil samples.
2. Collect 10 to 20 soil cores in a zig zag pattern across the entire area to be
sampled.
3. Collect soil cores from areas of similar soil texture and cropping history. If
different crops or markedly different soil textures occur in the same field, sample
them separately.
4. Collect to a depth of 6 to 8 inches around the plant root system (if present).
5. Bulk the cores in a container and mix thoroughly.
6. Place approximately 1 pint of mixed soil in a Midwest Labs soil bag and label
the outside of the bag.
7. Store the sample away from sunlight in a cool area until it is shipped to the
laboratory.
Collect 10-20 soil cores from a 18-20 acre area. Subdivide larger areas and sample
separately before bulking the soil into a single sample.

5. Additional Soil Sampling Info
For fields infested with Soybean Cyst Nematodes (H. glycines), run samples
before any SCN susceptible varieties are grown, or once a year if resistant
varieties are grown in rotation.
Sample Sugar Beet Cyst Nematodes (H. schachtii) injured fields once a year
regardless of crop choice or insecticide/nematicide use.
It is best to sample when plants are present. Cyst numbers will be highest when the
plants are almost mature to shortly after harvest. Sampling near harvest will lend
you plenty of time for variety selection or choosing alternative crops for the next
year.
Subdivide large fields into sections of 20 acres or less and make a single
composite sample per section to be submitted for analysis.
If the crop row is identifiable, place the soil probe within 2 inches of the row when
collecting the sample. Placement of the soil probe is not important when sampling
cultivated fields, or fields where soybeans were drilled.
The quality and condition of the sample determines the reliability of the results.
How to Deal with Hot Spots
Collect soil samples from the area between the damaged and the healthy plants. If
samples are collected from the center of the affected are, Cyst numbers may
appear much lower than their actual number.

6. Sample Shipment (Interstate)
Submittal forms are available online or by request.
https://www.midwestlabs.com/cgi-bin/subforms/subform_creator.pl?form_name=plant_parasitic_nematode
Submittal forms should be completed as follows:
• Name
• Address
• Phone Number
• State and County in which the field is located
• Date the field was sampled.
• Crop history (2-4 years)
Sample Shipment (cont.)
• Name or number of the field
Cyst nematode egg counts can be performed from the same sample as the basic
soil analysis if needed. Please use a soil analysis sub-form (autosubmit is
available) and select the cyst nematode egg count check box as well as the basic
soil test selection.
Sample Shipment (International)
Prior to shipping, contact Midwest Laboratories for shipping guidelines and proper
shipping labels.
Sample Results
Cyst nematode results can be viewed on our website at www.midwestlabs.com.
Enter your account number and password. If you do not have an account and
password, please contact our soil administrative personnel at (402) 829-9857.
Click on “SOILS” under the header “ACCESS MY DATA.”
On the left hand side of the page, click on “SAMPLE TYPES” and click on “Cyst
Nematodes.”
Click on the report number for applicable grower. When the view options
populate, click on “View Cyst Nematodes.”

7. For additional information, or to obtain a copy of the Midwest Laboratories
USDA/APHIS Permit, please contact:
George Nelsen, Nematology Supervisor
Phone: (402) 829-9868
gnelsen@midwestlabs.com
The USDA/APHIS permit can also be found by going to:
https://www.midwestlabs.com/midwest-labs-documents/

8. Soybean Cyst Nematode (SCN) Management Recommendations
Sampling Time
Infestation
Category
Following
Soybeans
Fall or Spring
(or soybeans
actively growing)
Following
Corn
Fall or Spring
(No soybeans
growing)
Management Recommendations
No SCN eggs
detected
0 0
No management strategies are necessary.
However, not finding SCN in a soil sample does
not prove that is not present in the field. Follow-
up sampling is recommended to check for SCN
infestations in future years.
Low* 1-4,000 1-2,000
If this is the first discovery of SCN, follow rotation
starting with Year 1 the next time soybeans are to
be grown. If Years 1-4 of rotation described below
have already been completed continue with Year 5
of the rotation.
Moderate* 4,001-16,000 2,000-12,000
Begin Year 1 of rotation the next time soybeans are
to be grown.
High* > 16,000 > 12,000
Grow several years of nonhost crop and sample
field again every fall to monitor decrease in SCN
population densities.
*Note: Egg counts are reported as eggs per 100cc (about ½ cup) of soil and are only estimations of actual SCN population densities.
6-year Crop Rotation Sequence
Year 1 Pl 88788 SCN-Resistant Soybean
Year 2 Nonhost Crop (such as corn, oats, alfalfa
Year 3 Peking SCN-Resistant Soybean
Year 4 Nonhost Crop (such as corn, oats, alfalfa)
Year 5 SCN-Susceptible Soybean
Year 6 Nonhost crop (such as corn, oats, alfalfa)
Repeat Six Year Roatation Sequence
What could you do if you cannot find any SCN-resistant soybean varieties
with Peking as a source of resistance? If an appropriate Peking SCN-resistant
soybean variety is not available for use in Year 3, grow a soybean variety with
SCN resistance derived from Pl 88788 that is different from the Pl 88788 SCN-
resistant soybean variety that was grown in Year 1. Grow the exact sample Pl
88788 SCN-resistant soybean variety in Years 1 and 3 only if no other Peking or
Pl 88788 SCN-resistant soybean varieties are available.

9. Sugar Beet Cyst Nematode Action Levels
NOTE:
Results from Midwest Laboratories, Inc. are reported as eggs per 100cc (about ½
cup) of soil and are only estimations of actual sugar beet cyst population densities.
Action Levels:
300 Eggs per 100cc
Rotation with non-host crops: The most widely used method of control is
rotation out of sugar beets with a non-host crop. Rotation with non-host crops
such as wheat, barley, corn, beans, or alfalfa will reduce the soil population of
sugar beet nematodes though natural decline. However, weed hosts must be
controlled during the rotation.
Information obtained from University of Nebraska-Lincoln
Midwest Laboratories, Inc.
13611 B Street
Omaha, NE 68144
(402) 334-7770 Phone
(402) 334-9121 Fax
*Reviewed and Updated 5/2015