1. CoolProducts
Organized. Protected. Standardized.

2. CoolProducts
CoolRackTM CoolBoxTM CoolCellTM
Modular thermo- Ice-free cooling and Alcohol-free cell
conductive tube and freezing freezing container
plate holders
Modular tools for cooling, (snap)freezing and
thawing biomedical samples
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3. Does this look familiar?
Most researchers still entrust their
precious samples to this type of cooling
method
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4. Here’s the same bucket again….
But in this one, the samples
are safely placed in one of
our CoolRack modules
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5. Why are CoolProducts necessary?
Worldwide effort to develop new standards for
collecting, handling, and processing pre-analytical
samples (blood, tissue, tumor, other biological
specimens or sample materials)
EU: http://www.devicelink.com/ivdt/archive/09/04/003.html
NCI/NIH: http://biospecimens.cancer.gov/practices/default.asp
“The lack of standardized, high-quality biospecimens has been widely
recognized as one of the most significant roadblocks to the progress of cancer
research.”
NIH Office of Biorepositories and Biospecimen Research 2010
Symposium: http://www.brnsymposium.com/meeting/brnsymposium/2010/
Duke: http://web.genome.duke.edu/cores/biorepository/protocols/
Molecular Epidemiology Workshop, Shanghai, 2008
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6. It’s all about the curves….
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7. ….the temperature curves
Each sample goes through the 0oC transition the same way.
Cool, freeze and thaw - identical each and every time
Consistent curves mean temperature control and experimental
repeatability…..INSTANT STANDARDIZATION
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8. CoolProducts
CoolRackTM CoolBoxTM CoolCellTM
Modular thermo- Ice-free cooling and Alcohol-free cell
conductive tube and freezing freezing container
plate holders
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9. CoolRack
Versatility
- rapidly adapts to any temperature
- can be used for a wide temperature range
- from -150oC through +200oC
Consistency & Standardization
- uniform temperature profiles for all samples
- inexpensive, comprehensive modular solution
-CoolRacks, CoolSinks, CoolBox, CoolTrays
- reduced user error
- standardize
- assay to assay, user to user, lab to lab, site to site
Organization
- indexed & organized sample management
- labels stay dry & legible
- minimize risk of contamination
Better sample organization
Patented novel alloy construction
& temperature consistency
- very rapid thermo-conductivity/adaptivity
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10. CoolRack
Tube holders
- some with profile-fit
Plate holders
- multi-well & PCR
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Platforms
- rectangular & round
Modular system is customizable, scalable and versatile
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11. CoolRack
-150oC -78oC 0o to 4oC 37oC +
Liquid Nitrogen Dry Ice Ice Water Bath
• Eliminate direct immersion • Replaces direct contact with • Gain organization & stability • Keeps tubes out of water –
into LN2 dry ice in ice reduced contamination, labels
• All tubes are upright • Eliminates ethanol bath on • Even temperature distribution stay dry
• Same freezing profile for all the bench – cost savings, to all samples even as ice melt • Consistent thawing profile for
samples reduced hazard & waste • Labels stay dry and legible for all samples
• ThermalTray provides • CoolRack PF provides equal better sample identification • All wells at identical
elevation of samples & or faster snap-freezing than • Reduced risk of sample temperature - eliminates “edge
increased distance between ethanol slurry contamination effect” on multi-well plates
hands and LN2 • Standardized freezing - • Rapidly goes from ambient to • Stable support of tubes &
• Rapidly adapts from ambient minimized standard deviation in <4oC in 90 seconds plates
(25oC) to LN2 temp (-150oC) in freeze profiles •Using ThermalTray, 10-15
12-14 minutes • Improved work flow hours of <4oC with one pan of
• Adapts to -78oC in 5-7 ice
minutes
Temperature control without directly immersing samples
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12. CoolRack
Cooling - Ice
Ambient to < 4oC in 60-90 seconds
4-6 hours of use when placing CoolRack directly on
ice
Up to 14 hours cooling with a single pan of ice with
ThermalTray
Cooling rate on ice
Snap-Freezing – Dry Ice
Equal or better freeze rate than dry
ice/ethanol bath
No alcohol required – cost savings, reduced
hazard
Consistent freeze profile for all samples
Freeze rate on dry ice Standard deviation
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13. CoolProducts
CoolRackTM CoolBoxTM CoolCellTM
Modular thermo- Ice-free cooling and Alcohol-free cell
conductive tube and freezing freezing container
plate holders
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14. CoolBox
Ice-free cooling and freezing
- 4 hrs w/o lid, 10 hrs with lid (+2oC)
- 3 hrs w/o lid, 6 hrs with lid (-12oC)
Versatile
- five temperature options in one box using
cartridges, dry ice or LN2
- portable
- accommodates a variety of tubes & plates
Consistent temperature
+2oC
- all wells within 0.1oC regardless of position even
as cartridges thaw
Highly recommended
- for highly-sensitive applications such as PCR
and RNA, use in the hood, cleanroom,
vivarium, short transporting, bio-hazardous
containment
Portable ice-free
cooling or freezing
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15. CoolBox
-25oC with thermal
regulator pad placed
between CoolRack and
dry ice
Snap-freezing at -78oC
with CoolRack directly on
200cc dry ice
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16. CoolBox
CoolBox Gel-Filled Container
Temperature range: Temperature range:
-150oC through +150oC Fixed at -20oC or 0oC
Cooling Media: Cooling Media:
LN2, Dry ice, Wet ice, Cartridge Gel
Well-to-well Temperature Variance: Well-to-well Temperature Variance:
0.1oC Up to 4oC
Tube & Plate Formats: Tube & Plate Formats:
0.5ml conical, 1.5ml conical, 1.5-2.0ml 0.5ml conical, 1.5ml conical, PCR tubes
cylindrical, 12.5mm dia cryos, mass
spec tubes, PCR 96- and 384-well
plates, 6-, 12-, 24-, 48-, 96-well plates
Cooling Duration: Cooling Duration:
10h (2ºC), 6h (-12ºC), 5h (-25ºC), 8h (0ºC), 3h (-21ºC)
6h (-78ºC)
Down Time: Down-Time:
None – switch cartridge with another 4-6 hours to re-freeze gel-filled tube rack
frozen one from the freezer or replenish
dry ice, LN2
CoolBox is more versatile, more consistent
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17. CoolProducts
CoolRackTM CoolBoxTM CoolCellTM
Modular thermo- Ice-free cooling and Alcohol-free cell
conductive tube and freezing freezing container
plate holders
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18. CoolCell
Alcohol-free cell freezing
No hassle
No on-going expense
No variability
High reproducibility
Better cell viability
Faster work flow
CryoPrep System
everything you need for cell prep in the
hood – CoolRack CFT, CoolBox, CoolCell
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19. It’s all about the curves…..
Fusion time and cooling profiles are
highly reproducible from run to run
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20. Better cell viability
In primary human cells, CoolCell
significantly outperforms
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21. CoolCell
CoolCell Alcohol-Filled Container
No alcohol Requires 250ml alcohol
•No hazardous fluids •Replace alcohol every 5 uses (250ml)
•Solid core and insulation design •Keep track of # of uses
•No pre-cooling required •Alcohol degrades slightly each time it’s used
•Pre-cool alcohol in refrigerator
Very consistent -1oC/min rate Inconsistent freeze rate
•All vials have uniform freeze rate every •Each run is slightly different due to alcohol
time degradation
•Radially symmetric – design enables •Two circles of wells – different freeze rates for
consistency each circle
Cost savings ~$338/year maintenance per unit
•No maintenance cost •Change alcohol weekly (~$6/wk)
•Pays for itself quickly •Dispose haz waste (~$0.75/wk)
•10 units = $3,380 to use EVERY YEAR
Lid opens easily Screw cap very difficult to remove when
•Not cold to the touch when removing frozen
from the -80C freezer •Protective glove required when handling frozen
unit
Better cell viability Good cell viability
Ready to use again in 5 minutes Takes >1 hour for device to return to room
temp for re-use
Low impact on freezer Large thermal mass impacts local freezer
•1/3 the heat impact on freezer compared area
to alcohol-filled units •Large heat capacity removed from alcohol
impacts nearby samples
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22. Customer quotes
Stanford University
“We run a registry, in which large amounts of PBMCs are processed for long-term
cyropreservation. After testing the CoolCell out, we found slightly better cell viability (>90%)
than our current cell freezing containers, and there is no Isopropanol waste generated. Overall,
the CoolCell has proven to us to set a new bar in cryopreservation.”
Merck Pharmaceuticals
“I have already purchased 21 CoolCells, I have obtained a CoolBox, and I recently ordered a
96well flat bottom CoolBox system. All these will be used for our assays. I need one more
product to complete my collection of ice-free cooling devices. I already passed on my evaluation
of the CoolCell and the other groups are pretty excited and are going to use them.“
UC Berkeley
“Our lab at UC Berkeley recently tried out demo models for the CoolRackM90, CoolSink, and
Thermal Tray and we loved them and thought they were very useful. We were just amazed by
how quickly the Cool Rack chills our tubes within minutes while preventing the possibility of
water getting in and contaminating our samples. ……We decided to get all of them after trying
them out due to their effectiveness. Our lab manager has been looking for a company that was
offering such products and fortunately, we've the right place!”
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23. Genentech Amgen Pfizer Roche Harvard MIT
CalTech USCF Glaxo Bristol Myers Squibb Novartis
Translational Genomics Gilead Sciences Merck
MedImmune Genpharm Elan Pharmaceuticals
Centocor Boehringer Ingelheim Oncomed NIH/NCI
Takeda Pharmaceuticals CDC Covance XOMA
Quest Diagnostics Otsuka America Pharmaceuticals
Thank you.
Salk Institute Burnham Institute Scripps Institute
Stanford University Yale Genzyme EMD Serono
Shire HGT Alnylam Pharmaceuticals Cell-System
Jackson Labs Beth Israel Deaconess Hospital HHMI
Whitehead Institute Harvard Systems Biology
Michelle Nemits
Exelixis Charles River Labs Sanofi Pasteur Penn State
VP Sales & Business Development
Lawrence Berkeley Lab Batelle UC Berkeley
Mobile: 415-336-4415
Cedars Sinai Hospital Cleveland Clinic IDEXX Biogen
Perkin Elmer Genetics Blood Systems Research Inst.
BC Cancer Research Center USC Pacific Bioscience
UCLA Precision Therapeutics Northwestern
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