Kurtz biomanufacturing

Protein is Cash:State of the Art Curriculum to Support the Bioeconomy Mary Jane Kurtz, Ph.D NBC 2 Consultant [email_address]

Basis of the Bioeconomy ,[object Object],[object Object],[object Object]

Ten Technician Jobs Anchor Ten Biomanufacturing Departments ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Biomanufacturing/Biotechnology A plus for Teachers and Students ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

NBC 2 Products ,[object Object]

Growing the Local Biotech/Biomanufacturing Network across the Nation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Northeast Biomanufacturing Center Web Site ,[object Object],[object Object],[object Object]

RESEARCH & DEVELOPMENT(pre-clinical): Discovery Research OPERATIONS: Process development, Manufacturing & Production QUALITY: Quality Control & Assurance CLINICAL RESEARCH: Clinical Research: Regulatory Affairs Discovery Research Senior Scientist Scientist III,II,I Research Associate Process Development Director Supervisor & Process Development Technician Quality Control (QC) Clinical Research Clinical Research Manager Bioinformatics Scientist Engineer Analyst Programmer Manufacturing & Production Supervisor Associate Technician (Operator) Instrumentation Tech Calibration Technician Facilities Management Manager Facilities Technician Shipper/receiver Chemistry Chemistry QC Analyst QC technician Microbiology Microbiology QC analyst QC Technician Quality Assurance (QA) Documentation Specialist QA Documentation Coordinator Clinical Research Clinical Research Manager Regulatory Affairs Manager Associate Data Manager Business Development Director of Business Development Administration Human resources Safety Manager ,[object Object],[object Object],Entry level positions require an Assoc deg or certificate Higher entry levels require a BS, MS, PhD or Engineering degrees

Biomanufacturing Curriculum: National Standard Concepts Covered ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Career Tracks: Protein is Cash Introduces ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Biomanufacturing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

High School Curriculum A Workshop to Instruct Teachers and Students in Biomanufacturing and the Bioeconomy

Overview of Protein is Cash ,[object Object]

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Day 1 : Metrology/Instrumentation ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Day 2 Transformation of Bacteria ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Day 2 E.coli Transformation with pGLO plasmid Ampicillin resistance gene (Amp r ) and target gene on bacterial plasmid Bacterial clones Cell division Transformation mixture is plated on to agar plate containing Ampicillin Only E. coli containing plasmid survive on Ampicillin plates

Results of Inserting Foreign DNA into an Organism Cells will multiply and produce desired gene product pGlO gene expression vector: Green Fluorescent Protein

Day 2: Upstream Processing: Cell Growth and Lysis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Day 3: Downstream Processing: Purification of Green Fluorescent Protein by Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Isolation and Purification of Green Fluorescent Protein Transformed cells #1 #2 #3 Fraction number Test tubes Courtesy of Bio-Rad

Separation of Proteins by Column Chromatograph ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Size Exclusion Chromatography Red molecule =10^6 daltons - Blue molecule = 600 daltons

How Size Exclusion Works ,[object Object],[object Object],[object Object],[object Object]

Hydrophobic Interaction Chromatography ,[object Object],H+ H+ H+ H+ High salt: 2 M (NH 4 ) 2 SO 4 Wash buffer: 1.3 M (NH 4 ) 2 SO 4 Elution buffer: 10 mM Tris Hi Salt

Ion Exchange Chromatography ,[object Object],[object Object],[object Object],[object Object]

Cation Exchange vs Anion exchange ,[object Object]

Day 4: Quality Control ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Quality Control Analysis of Column Fractions ,[object Object],[object Object]

PAGE of pGLO samples after IEX chromatography A B C D E F G H I J A = molecular ladder B = no sample C = IEX #1 fraction column D = I EX # 2 fraction column E -G = IEX wash fractions H = Supernatant I = molecular ladder J = standard GFP 29,000 mol wt 29,000 mol wt

Day 5. Discovery Research and FDA approval process ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Bio-Rad Partnership ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

$ Protein is Cash $ Local Teachers Workshops Biomanonline.org

Protein is Cash Workshop Teaching Biomanufacturing in HS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

HTTP ,[object Object],[object Object]

Useful Information for Biomanufacturing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Kurtz biomanufacturing

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