Dr. Thomas Tubon from Madison College describes the process of developing a program in stem cell technology.

1. Development of a Technical
Program in Stem Cell Science:
Responding to an Emerging Need
National Science Foundation
Advanced Technological Education
DUE 1104210

2. Madison College
• 2012 Centennial
Celebration
•Over 140 career paths
serving more than 45,000
students, including many
of the fastest growing
occupations identified by
the WDWD.
•12 Campuses (8 in
Madison, 4 Regional).
•Greater than 90%
placement rate for
graduates within 6
months.
•Technical colleges
generate nearly $7 billion
in economic benefits for
Wisconsin each year.Facilities Expansion
Health Education
Ingenuity Center
Protective Services

3. Biotechnology in Wisconsin
• Between 2004 and 2009 bioscience employment in Wisconsin
grew by nearly 3% in contrast to the rest of
the economy that shrunk by > 3%
• The average bioscience worker has earnings that are 64%
higher than the earnings of a typical Wisconsin employee
• More than 640 Wisconsin bioscience businesses have
created nearly 24,000 private sector jobs with a
total economic impact of close to $7 billion

4. •Reported industry revenues in 2005 totaled $974,000 and $36.9
million in 2007. In the US, by 2016, stem cell company revenues
are projected to exceed $8.5 billion and $16-20 billion by 2020.
•The state of Wisconsin has invested more than $94 million, in
addition to private investments to promote growth in the stem cell
and regenerative biology sector.
•California Institute of Regenerative Medicine (CIRM) $3 billion in
Grants.
The Bioeconomic impact of
Stem Cell Science

5. Stem Cells & Regenerative Biology
At Madison College
• In 2007, Madison College piloted our first human stem cell
course.
•In 2011, NSF awarded $851,454 to develop a technical
education program in Emerging Stem Cell Technologies.
•40 Advisory Board Members: Stem Cell Industry, UW Madison
SCRMC, UW Waisman Center for Developmental Disorders,
and Morgridge Institute for Research / Wisconsin Institute for
Discovery.

6. Development of a Technical Program in Stem
Cell Technologies: Responding to an
Emerging Need (NSF DUE 1104210)
BJECTIVES:
I. Development of a 2-semester certificate program
emphasizing workforce training in Stem Cell Technologies
II. Develop educational materials in stem cell technologies for
dissemination at the local, regional, and national levels.
(web-based distribution, instructional videos, manuals)
III. Promote the growth of Stem Cell Programs in other Colleges
& Universities throughout the nation

7. Introduction to human Stem Cell Methods:
Basic Culture and Characterization
Course Competencies:
Characterization of hESCs
• Chromosomal analysis, florescence microscopy
• Media Formulation (Feeder-dependent/xeno-free media)
•Generation of Cell Aggregates (EBs)
hESC H9 DAPI Stain
For metaphase chromosomes
hESC H9 Cell Aggregates:
Embryoid formation

8. Scheduled Meeting time:
ecture: Monday 5:00pm to 5:50pm
aboratory: Monday/Thursday 6:00pm-8:50pm
arget audience:
AAS Biotechnology, BS in Biological Sciences at or nearing completion
Post-Baccalaureate, Graduate Students, Laboratory Staff.
Human Stem Cell Technologies Certificate
Program: 4 Courses (8 Credits)

9. SEMESTER 1: Introduction to human Stem Cells
Basic Culture and Characterization
Course Competencies:
• Stem Cells and Bioethics
• Aseptic techniques and routine maintenance of PSC
cell cultures.
• Basic techniques for culturing hES (H9) cells:
thawing, plating, feeding, passaging, and cell banking
Pluripotent Stem Cell colony
pre-split (10x)
Pluripotent Stem Cell colony
pre-split (2.5x)

10. Course Competencies:
• hES culture systems: Feeder dependent/independent.
hESC H9 Colony on
MEF-feeder layer (20x)
hESC H9 Colony – Feeder independent
On Matrigel (10x)
SEMESTER 1: Introduction to human Stem Cells
Basic Culture and Characterization

11. Course Competencies:
• hESC (H9) and iPS (iMR90-4) Cell Differentiation
Spontaneous & Directed Differentiation
of Adherent Cells and Cell Aggregates
•Immunoflourescence Microscopy & Molecular Analysis of
PSC-derived differentiated cells
SEMESTER 2: Advanced human Stem Cells-
Differentiation & Applications
Hepatocyte
Differentiation
Neural Rosette
Differentiation
Cardiomyocyte
Differentiation

12. Advanced Cell Culture Education Suite (ACCES)
•2744 sq.ft. Animal Cell Culture Facility.
•Construction completed and fully operational, January 2012.

13. Advanced Cell Culture Education Suite (ACCES)
Molecular Wet Lab Cell Culture Imaging & Microscopy

14. Neuronal Differentiation
Neural Differentiation of Human Embryonic Stem Cells
Anderson, B., Bagnall, J., Holston, N., Jefferson, K., Ledbury, B., Rosenbaum, H., Schmidt, C.,
Schreiber, S., Skinner, J., Steckenfinger, S., Stephens, D., and Sugden, D.
Madison Area Technical College, Biotechnology Program
Human Stem Cell Education Certificate
1701 Wright St. Madison, Wisconsin 53704 (608)246-6875
•Utilizing the Zhang Protocol, we were able to differentiate human embryonic stem
cells towards ectodermal derivatives and further to neural cell-types.
•Verification by cell morphology and immunocytochemistry demonstrates that the stem
cells followed the expected pathway for neural differentiation.
•The next step is to perform RT-qPCR for confirmation of specific gene expression,
including Pax6 and β-tubulin.
•The cells are still in the process of culturing, and will continue down the neural
lineage pathway until they form mature neural networks.
Support for the development of the StemCell Education Initiative isprovided through aNational
Science FoundationAdvanced Technological Education Grant (DUE1104210)awarded to Madison
College. Aspecial thanksto WiCell, CDI, &Life Technologiesfor providingcells and reagentsto
support our program.
Conclusions Reference
Acknowledgments
Introduction
Emerging stem cell science provide the basis for today’s most promising technologies with far reaching implications in both
research and medicine. Current and potential applications include: replacement of damaged or diseased tissue, studying
human development, testing of new drugs, screening for potential toxins, developing new methods for gene therapy, and
likely many other yet to be determined future uses. As the field of stem cells and regenerative medicine continues to
evolve, our understanding of the science leads into methods of cell differentiation into even more specific cell types which
were previously not possible. This paradigm shift will undoubtedly change the way medicine is performed from the
conventional methods used today allowing us to precisely target and replace damaged, malfunctioning, and aging cells.
DUE 1104210
Results
H9 Cultured on
MEFs
Day 3:
EBs Formation
in NIM
Day 10:
Early Rosette
formation
Day 14 :
Late Rosette
Formation
Day 24:
Neurospheres
in NDM
Day 36:
Immature
Neurons
Pluripotent StemCell Media(PSC):
•DMEM/F12
•KOSR
•MEM NEAA
•bFGF
L-Glutamine + BME
Neural Induction Media (NIM):
•DMEM/F12 Glutamax
•NEAA
•Heparin
•N2 Supplement
Neural Differentiation Media (NDM)
•Neurobasal Media
•Glutamax
•30% Glucose
•B27
•cAMP
•Ascorbic Acid
•N2 Supplement
•GDNF, BDNF, and NT3
Media PreparationsMethods
Directed differentiation was achieved by growth in selective
media preparations NIM and NDM, according to standard
formulation (Hu, B and Zhang, S.C, 2010)
Pax6 DAPI
BetaTubulin DAPI
Hu, Bao-Yang and Su-Chun Zhang, 2010. Methods Mol Biol. Directed
differentiation of neural-stem cells and subtlype-specific neurons from hESCs,
636, 123-127
Day 42
Phase contrast
2013 Biopharmaceutical Technical
Center Institute
STEM CELL SYMPOSIUM

15. Summary : Stem Cell Education
With NSF ATE support, we have developed a
technical training program in Human Stem Cells
to address the emerging demands of the global
bioeconomy. Programming also includes the
creation of 1-day workshops & a 5-day Short
Course

16. Stem Cells & Regenerative Biology
In Madison, Wisconsin

17. Thank you!
Supported by the National Science Foundation Advanced
Technological Education Project Grant DUE 1104210
Awarded to Madison College July 2011

18. Contact Information
•Lisa Seidman, Ph.D
PI, Biotechnology Program Director
(608) 246-6204
lseidman@matcmadison.edu
•Thomas Tubon, Ph.D.
Project Director/Co-PI, NSF Stem Cell Program at Madison Colleg
(608)246-6875
tubon@matcmadison.edu
•Jeanette Mowery, Ph.D.
Co-PI, Faculty
jmowery@matcmadison.edu