1. OSTEOBLAST
RESPONSE TO
INDIGENOUS
DEMINERALISED
BONE MATRIX

2. Presentation by
Dr.S. Thanga Kumaran
Post graduate
Ragas Dental College & Hospital
Chennai
Guided by
Dr .K.V.Arun M.D.S.,
Department of Periodontics
Ragas Dental College & Hospital
Chennai

3. • Regeneration of lost periodontal apparatus
is the ultimate goal of periodontal therapy.
• Bone replacement graft’s(BRG) have been
widely used to achieve this regeneration
with varied results.
• Osteo inductive bone grafts are thought to
be clinically superior because of their
ability to induce new bone formation from
uncommitted progenitor cells.

4. Experimental evidence of osteo induction have
come from the following
1. Animal studies showing ectopic bone
formation following subcutaneous and
intramuscular implantation of
osteoinductive grafts (Zhang et al)
2. In vitro studies of osteoblastic and
osteoclastic proliferation following
exposure to inductive material.

5. BONE MARROW
ISOLATION

12. BONE MARROW
TRANSFER

34. CELL PROLIFERATION

35. CONTROL

36. OSSEOGRAFT

37. GRAFTON

38. CELL
PROLIFERATION
CONTROL
CONTROL
GRAFTON
OSSEOGRAFT

39. CELL PROLIFERATION
CONTROL OSSEOGRAFT GRAFTON
DAY 5 ++ + ---
DAY 7 ++ ++ ++
DAY 8 ++ +++ +++

40. CELL DIFFERENTIATION

47. CONTROL

48. OSSEOGRAFT

49. GRAFTON

50. CELL DIFFERENTIATION
CONTROL
GRAFTON
OSSEOGRAFT

51. ALKALINE PHOSPHATASE (ALP)
ACTIVITY
Grafton
Control Osseograft Grafton

52. CONCLUSION

53. Grafton and osseograft both
have the potential for stimulating
osteoblastic proliferation and
differentiation from
undifferentiated mesenchymal
cells and hence both are truly
osteoinductive in nature.