Umbilical Cord blood stem cell is obtain from biological waste from which we can treat over 80 diseases grt inovation in science..........

1. UMBILICAL CORD STEM CELL
PRESENTED BY :- SINGH ABHISHEK.C
Department of Biotechnology
Ismail Yusuf college
Mumbai University

2. INTRODUCTION
• Blood remaining in the
umbilical cord and placenta
after birth is called as
Umbilical Cord Blood stem
cell is also known as Cord
Blood.
• The blood is rich in stem
cells derived from the
Umbilical Cord and
placenta after birth.
• After the birth Umbilical
Cord Blood taken from
umbilical vein after cord is
cut and clamped.
• UCB blood can be stored
for 25 years.

3. • During prenatal development,
the umbilical cord is
physiologically and genetically
part of the fetus and (in humans)
normally contains two arteries
(the umbilical arteries) and one
vein (the umbilical vein), buried
within Wharton's jelly.
•The umbilical vein supplies the
fetus with oxygenated, nutrientrich blood from the placenta.
•Conversely, the fetal heart pumps
deoxygenated, nutrient-depleted Cross section of umbilical cord. Top right
blood through the umbilical
and top left: umbilical artery, bottom:
umbilical vein, middle: remnant
arteries back to the placenta
of allantois.

4. History
• 1974 First report on stem/progenitor
cells in human cord blood.
• 1988 First successful cord blood
transplant to regenerate blood and
immune cells in Paris, France on a sixyear-old boy suffering from Fanconi's
Anemia, a blood disorder donor was his
identical sister.
• At the time of the first transplant, little
was known about the biologic properties
of CB cells and it was thanks to the
pioneering work of H. E. Broxmeyer and
E. A. Boyse, who studied the progenitor
cell content of CB.
• 1992 New York Blood Center establishes
first public CB bank.
H. E. Broxmeyer
Edward .A. Boyse

5. How to collect UCB cells
Blood sample in
biohazardous bag
Blood sample
Pregnant Mother
Diagnostic test for diseases.
 HIV
HEPATITIS B
HEPATITIS C
SYPHILIS
HTLV
CMV

6. Healthy baby
Cord blood collection

7. How to preserve CB
• After the collection, the cord blood
unit is shipped to the lab and
processed, and then
cryopreserved.
• However the unit is processed, a
cryopreservant is added to the
cord blood to allow the cells to
survive the cryogenic process.
• cryoprotectants such as glycerol
or dimethyl sulphoxide.
• After cryogenicprotectants added
blood unit should be placed in
freezer or extra cold vessel
• Eg :- Nitrogen liquid (-196 degree
celsius)

8. CB blood contain
• CB blood contain stem
cell which is also known
as haematopoietic cell or
progenitor cell.
• This stem cell further
turn into different cells,
like RBC, WBC,
Monocytes,
Lymphocytes, Platelet, Bcell, T-cell, NK- cell etc.
• This stem cell help to
cure 80+ diseases.

9. APPLICATION
Stem cells can be stored and used to treat
over 80+ diseases including leukemia,
lymphoma and more.

10. CB Bank in India
• RELIANCE LIFE SCIENCE First public CB
bank(2002).
• LIFE CELL INDIAS First CB bank (2004).
• Cryobank International associated with RJ crop
and founded Cryobank India (2006)
• Stemcyte Inc. USA associated with Apollo hospital
Ahmadabad found stemcyte India(2007).
• Jeevan stem cell bank public as well as private.
• CordCare bank founded by Pathcare pvt. Ltd is
public CB bank.

11. CB price in India
• Not much difference in
price to preserve CB by
different CB bank.
• Minimum 70,000 and
maximum 1,50,000
Rupees.
• Its very cheap and
amazing gift from
parents to their
children or baby.

12. Difference between private and public
bank
Cord Blood Banking (Private)
Cord Blood Banking (Public)
• Costs $1000-2000 initially
+ ~$100/year
• Ownership of the blood
retained by the family
•
Cost: free
• Blood donated and not
reserved for child or family
• May be used for research in
some instances (read informed
consent)

13. Some Transplant Centres in India
• Tata Memorial Hospital,
Mumbai
• Adyar Cancer Centre, Chennai
• Apollo Specialty Hospital,
Chennai
• Apollo Hospital, Hyderabad
• Christian Medical College,
Vellore
• R&R Army Hospital, New Delhi
• AIIMS, New Delhi
• Inlaks Hospital, Pune
• Armed Forces Medical College,
Pune
• Sanjay Gandhi PGIMS, Lucknow.

14. Most Recent Application In India
1.Diabetes
(National Centre for Cell Sciences, Pune University –
Dr. Ramesh Bhonde)
2.Heart (Dr. Venugopal Rao – AIIMS)
3.Liver Disease
4.Parkinson's Disease (Manipal Dr. Satish Totey)
5.Spinal Cord Injury (AFMC, Pune)
6.Stroke
7.Alzheimer's Disease

15. Something About HLA Test
Definition
• The human leukocyte antigen test, also known as HLA, is a
test that detects antigens (genetic markers) on white blood
cells. There are four types of human leukocyte antigens: HLAA, HLA-B, HLA-C, and HLA-D.
Purpose
• The HLA test is used to provide evidence of tissue
compatibility typing of tissue recipients and donors. It is also
an aid in genetic counseling and in paternity testing.
Precautions
• This test may have to be postponed if the patient has recently
undergone a transfusion.

16. •Preparation
The HLA test requires a blood sample. There is no need for the
patient to be fasting (having nothing to eat or drink) before the
test.
•Risks
Risks for this test are minimal, but may include slight bleeding
from the blood-drawing site, fainting or feeling lightheaded
after venipuncture, or hematoma (blood accumulating under
the puncture site).

17. Advantages
• Cord blood is a rich, natural, controversy-free source of lifesaving stem cells.
• The collection of cord blood does not harm the mother or
child, and does not interfere with the birthing process.
• Umbilical cord blood has the potential to cure over 80
diseases.
• Recent research has shown tremendous potential for
cord blood stem cells to be used in regenerative medicine,
which may cure even more diseases.
• cord blood is readily available when needed, if collected and
banked.
• Cord blood stem cells are the “youngest” and most adaptable
form of cells, and the body more readily accepts the cells into
its system.

18. • Cord blood has a broader match potential than bone marrow,
which means that the patient and donor do not have to be a
“perfect match.”
•Cord blood stem cells are more resistant to infection, have
fewer side effects after the transplant and require fewer
transplant drugs than bone marrow stem cells.
•There are nearly 10 times as many blood producing cells in
cord blood. Studies suggest that cord blood may also have a
better ability to generate blood cells than bone marrow. This
provides more opportunity for future medical advancements.

19. Disadvantages
• The main disadvantage of cord blood is that the volume
collected is fixed and relatively small.
• Therefore, the number of stem cells available for
transplantation is low compared to the number of cells that
can be collected in customizable bone marrow or peripheral
blood stem cell harvests.
• It’s a very costly in private bank.
• Risk of high contamination .
• High risk to be missmatch genetic typing.

20. CONCLUSION
• Source of stem cells from
biological waste.
• Collection of cord stem cells is
painless.
• Collection of cord stem cells is risk
free to mother and baby.
• Cord blood stem cells are much
more tolerant to HLA tissue
mismatching than bone marrow
therefore leading to lower rate.
• Cord blood stem cells have a
greater ability to differentiate into
other cell types.
• Save Cord blood save your future
• Cord blood stem cells are being
used in the treatment of 40
medical conditions with over 72
potential disease targets

21. REFFERENCE
•
•
•
•
Umbilical cord blood wikkipedia
Placenta wikkipedia
Umbilical Cord wikkipedia
Americord Bank (US)
• Jayanti Tokas, Puneet Tokas, Rubina Begum, Shailini Jain
and Hariom Yadav
Department of Biotechnology, JMIT, Radaur, Haryana,
India KITM, Kurukshetra, Haryana, India

22. • Christina Bemrich(Tinsley Harrison Research Society).
• Elisabeth Semple, PhD Scientific Director
Cells for Life Cord Blood Institute Toronto.
• Cryo-Save India .com
•Relicord.com
•Jeevan stem cell bank.
•Life cell stem cell
•Stemcyte .com
•parentsguidecordblood.org
•bmt.umn.edu
health.india.com
•medical-dictionary.thefreedictionary.com
•cordblood.findthebest.com
•www.alphacord.com
•www.newindianexpress.com
•www.cordcareindia.com
•www.pharmabiz.com
•www.slideshare.net