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Separation Techniques and
Enterance to
Chromatography
Prepared by :ABDULHAMID MOHAMED
Student no: 1302d901
YILDIZ TECHNICAL UNIVERSITY
Methods of Separating Components of
Mixtures
Separation Techniques
• The separation of mixtures may be
done to enhance the purity of
substances
• Accomplished using different
characteristic properties, such as
density, boiling point, melting point,
solubility, etc
Some Types of
Separation Techniques
– filtration
– mechanical separation
– floatation
– centrifugation
– simple distillation
– fractional distillation
– crystallization
– chromatography
Filtration
• Technique
used to
separate
mixtures of an
insoluble solid
and a liquid.
Examples of Mixtures You Can
Separate Using Filtration
• Sand and water
• Broken glass and water
Mechanical Separation
• Physical separation methods that
involve the use of tools such as forceps
and sieves, to separate the components
of a mixture.
• Ex. Gravel and sand
Flotation
Separation method
in which some
solids of a
suspension mixture
are allowed to
settle and the less
dense material is
poured off.
Ex. Panning of gold
Centrifugation
• Speeds up the
separation of a solid
from a liquid
through the
centripetal force
developed during
the rotation of the
centrifuge.
Distillation
• This is the separation technique where
two miscible liquids (liquids that mix
together) are separated.
• It is made possible due to the fact that
each liquid has its unique boiling point.
Types of Distillation
• Simple distillation
• Fractional distillation
Simple Distillation
• This is a technique
used to separate a
mixture of a
soluble substance
and a solvent.
• E.g salt and water
Fractional distillation
• This is the technique used to
separate a mixture of two miscible
liquids with different boiling points.
• E.g. water and ethanol
• The boiling point for water is 100
while for ethanol it is 78.
Fractional Distillation Demonstration
Fractional Crystallization
• Crystallization is the process in
which crystals are formed.
• Fractional crystallization is done by
lowering of temperature of a
mixture or solution so that the more
insoluble component crystallizes
out first. (Uses solubility to
separate substances)
Chromatography
• Is the separation technique used to
separate soluble substances using a
media and a solvent.
• It is mostly applied in identifying
mixtures that are colored or pigments.
Some Types of
Chromatography
• Radial chromatography
• Ascending chromatography
Radial Chromatography
• In this type of chromatography, as the
pigment separates, the different colors
move outwards.
Radial Chromatogram
Ascending Chromatography
• The solvent
moves
upwards on the
separating
media

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Separation techniques and enterance to chromatography