This document summarizes research on vitamin B12 deficiency caused by consumption of reverse osmosis (RO) water. It finds that RO water filters out minerals needed to absorb vitamin B12. A lack of vitamin B12 can cause issues like back/knee pain, fatigue, poor concentration, appetite loss, shortness of breath, and nerve damage. The research team distributed questionnaires to 100 people to determine if those drinking RO water experienced more B12 deficiency symptoms. Their results from the questionnaires suggested that half had poor concentration, 60% felt fatigue/weakness, 60% felt low mood/depression, 62% felt dizziness, and 55% had frequent headaches.

1. Research on deficiency of Vitamin
B-12 due to consumption of RO
water
Prepared by – Abbas Miyajiwala
(01)
Alvi Patel
(02)
Aman Goswami
(03)
Anirudh Chauhan
(04)

2. Causes for deficiency of Vitamin B-12
 Water treated by reverse osmosis (RO) filters out
minerals that help to assimilate vital nutrients and
produce vitamin B-12.
 Vitamin B-12 deficiency can cause various health
problems like constant back pain, knee pain, fatigue,
lack of concentration, loss of appetite, shortness of
breath, bleeding gums and even nerve damage
 The second reason could be that B-12 isn’t easily
absorbed by the stomach.

3. Importance of Vitamin B-12
 Vitamin B-12 is helpful to the body to keep its nerves
and blood cells healthy.
 It is also helpful in formation of healthy DNA.
 It is water soluble mineral and is main source of mineral
Cobalt.

4. Special Thanks to…
 Dr Bharat Dave, honorary spine surgeon at VS Hospital
and NHL Medical College, helped us significantly to
conduct our research.
 Not only he provided us with his precious time, but also
guided us in forming and designing our questionnaire.

5. Research Design
 The main objective of conducting this research was to
find relativity between Depletion of Vitamin B-12 and
Consumption of RO Water.
 A Questionnaire containing 19 questions was
prepared and data was collected from 100 individuals.
 Qualitative Approach

6. Cont…
Hypothesis:
Null hypothesis (Ho) - There is association of deficiency
of vitamin B-12 with regular consumption of RO treated
water.
Alternate hypothesis (Ha) - There is no association of
deficiency of vitamin B-12 with regular consumption of
RO treated water.

7. Cont…
Phases of Research: Research was divided into 3 phases
Phase 1 : Questionnaire were distributed among all 4
members to collect data. The team was
guided to keep patience throughout the data
collection by making them realize the degree of
stress involved in it.
Phase 2 : Data collected was transferred into Excel to get
necessary interpretations.
Phase 3 : Bar charts were developed to know the impact of
different problems included in research.

9. Yes
50%
No
50%
Interpretation: The prolonged deficiency of vitamin B-12
causes loose memory and lack of concentration.
Half of the sample population had complaint
regarding this issue.
Poor-Concentration / Memory

10. Yes
60%
No
40%
Interpretation: Deficiency of Vitamin B-12 leads to megaloblastic anemia
due to which the patient/person feels exhaustion and weakness
throughout the day. Sometimes exhaustion is also
accompanied with body ache. Majority of population
complained regarding exhaustion and weakness.
Fatigue / Weakness

11. Low Mood / Depression
Yes
60%
No
40%
Interpretation: Vitamin B-12 and other B vitamins play a role in producing
brain chemicals that affect mood and other brain functions. Low
levels of B-12 and other B vitamins such as vitamin B-6 may be
linked to depression.

12. Dizziness / Lightheadness
yes
62%
No
38%
Interpretation: Dizziness is the feeling of being lightheaded, woozy, or
unbalanced. It affects the sensory organs, specifically eyes and ears.
It can cause fainting. Dizziness is not a disease but a symptom of
other disorders. Pernicious anemia caused by vitamin b-12 depletion
can be the major disorder.

13. Daily / Frequent Headache
Yes
55%
NO
45%
Interpretation: Frequent headaches are mostly common among the
patients of Vitamin B12 deficiency. Majority of sample population
had this complain. Reasons behind headache are mainly
gas, bloating, hyperacidity , non-restorative sleep all
of which have only one root and i.e. deficiency of vitamin B12.

14. Thank You