Microfluidic technologies for Sample Preparation Discover the presentation held by Benjamin Roussel on LOAC Asia 2015 in Singapore presentation by Yole Développement A common path among all different types of assays SAMPLE PREPARATION IN IN-VITRO DIAGNOSTICS Often mandatory, always challenging Sample preparation is challenging HOW TO DEFINE NECESSARY SAMPLE PREP STEPS? Defining the appropriate workflow FIRST LEVEL PACKAGE MICROFLUIDIC DEVICE MARKET FOR SP Total Accessible Market without SP for Immunoassays

1. November© 2015
From Technologies to Market
Microfluidic
technologies for
Sample
Preparation
LOAC ASIA 2015
Benjamin ROUSSEL
From Technologies to Market
Image: courtesy of Bio-Rad

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PRESENTATION OUTLOOK
15 + 5 mins
• Yole Développement Introduction
• What is sample preparation ?
• Current bottlenecks and challenges
• Microfluidic for Sample Preparation
• Q&A

3. © 2015
Yole Développement
FromTechnologies to Market

4. 4
MEMS &
Sensors
LED
Compound
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Imaging Photonics
MedTech
Manufacturing
Advanced
Packaging
PV
Power
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Yole Développement’s 30 analysts operate in the following areas
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OUR GLOBAL ACTIVITY
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YOLE ACTIVITES
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Microfluidics for Sample Prep
©2015 | www.yole.fr | Microfluidic technologies for sample preparation

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SAMPLE PREPARATION IN IN-VITRO DIAGNOSTICS
What is sample preparation?
Introduction
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample
Collection
Detection
Whole Blood
Serum
Plasma
Urine
Swab
Food sample
Crop Water
Electrochemical
detection
Optical detection
Acoustic detection
Sample nature
Type of detection
SAMPLE PREPARATION

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HOW TO DEFINE NECESSARY SAMPLE PREP STEPS?
General steps
A common
path among all
different types
of assays
Sample
preparation
main
objectives
Sample collection
and stabilization
1
Extraction of the
analyte of interest
(e.g. cell lysis)
2
Removal of the
interferences and inhibitors,
isolation and protection of
the analyte
« Sample purification »
3
Labelling
5
Detection
6
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
4
Adaptation of the
sample properties (pH,
concentration…) and/or
amplification (e.g. PCR)

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HOW TO DEFINE NECESSARY SAMPLE PREP STEPS?
Defining the appropriate workflow
SP Workflow =
combination of
functions
Mixing
Stirring
Heating
Dilution
SedimentationConcentration
Shearing
Separation
Fusion
Amplification
Cooling
Washing
Precipi
-tation
pH adjustment
Elution
Labeling
Degassing
Extraction Cell Lysis
Dissolution
Homogenizati
on

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SAMPLE PREPARATION IN IN-VITRO DIAGNOSTICS
Often mandatory, always challenging
Sample
preparation
is challenging
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Time
consuming
Important
hands-on time
Equipment 1
Equipment I1
Equipment I1I
Equipment IV
Multi
Equipment
Often
expensing

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WHY MICROFLUIDICS IS OF INTEREST FOR SAMPLE PREP
Automation
is key
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Microfluidics allows
AUTOMATION
Avoid manipulation Enable use by untrained people
Reduce or suppress hands-on timeReduce the amounts of reagents
Reduce environment exposureReduce risk of contamination
Reduce variability Increase reproducibility

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MICROFLUIDICS FIND APPLICATIONS IN DIFFERENT FIELD
Field of
applications
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample prep
for Molecular
Diagnostics
(Including Digital
PCR)
Sample prep for
Single Cell
Sample prep for
Next Generation
Sequencing
Sample prep for
immunoassays
Focus of today’s presentations

14. © 2015
Market perspective
©2015 | www.yole.fr | Microfluidic technologies for sample preparation

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MICROFLUIDICS HISTORY
Sample prep
is now very
critical
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample
collection
• Solid
• Swab
• Water solution
• Blood
• …
Sample
Injection
• Interface Micro-
Macro
• Connectors
• Air bubbles
• …
Sample
preparation
• Mixing
(reagents)
• purification,
filtration
• Concentration
• Lysis
• …
Amplification
• PCR
• qPCR
Detection
• Optical
• Fluorescence
• Electro-
chemical
• …
Most Microfluidic developments
have focused on detection
phase
Lack of technologies
for sample preparation step

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A MAJOR MILESTONE FOR THE MICROFLUIDICS INDUSTRY
Market
adoption
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
TIME
1990 2000 2015 2020?
Adoption of microfluidic technologies
for Sample prep
Adoption of microfluidics
technologies for detection

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NGS EXAMPLE
NGS
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample
collection
• Solid
• Swab
• Water solution
• Blood
• …
Sample
Injection
• Interface Micro-
Macro
• Connectors
• Air bubbles
• …
Sample
preparation
• Mixing
(reagents)
• purification,
filtration
• Concentration
• Lysis
• …
Amplification
• PCR
• qPCR
Detection
• Optical
• Fluorescence
• Electro-
chemical
• …
2015 2000’s

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THE 2015-2016 TRANSITION IS AN INFLECTION POINT!
Sample prep
market
breakdown
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
The growth of microfluidics for single cell will slow down in 2017, meanwhile the
microfluidics for genomics sample preparation market will continue an impressive growth

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FIRST LEVEL PACKAGE MICROFLUIDIC DEVICE MARKET FOR SP
Total Accessible Market without SP for Immunoassays
It’s just the
beggining
©2015 | www.yole.fr | Final report
0
50
100
150
200
250
300
350
400
2014 2015 2016 2017 2018 2019 2020
Microfluidics for Sample Prep Market ($M)
Microfluidics for Molecular
Diagnostics Sample
Preparation
Microfluidics for Genomics
Sample Preparation
Microfluidics for Single Cell

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SINGLE CELL SAMPLE PREP
Through microfluidics technologies
Single cell
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample prep for
Single Cell
Some of the key drivers:
- Throughput
- Hands-on time
- Temperature control
- Shear stress reduction

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NGS SAMPLE PREP
Through microfluidics technologies
Next
generation
sequencing
©2015 | www.yole.fr | Microfluidic technologies for sample preparation
Sample prep for
NGS
Some of the key drivers:
- Throughput
- Flexibility
- Type of sample accepted

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CONCLUSIONS
• During years, microfluidics technologies have focused on the detection phase solving the
bottlenecks in term of specificity, sensitivity, portability…
• Today, the main remaining challenges for IVD companies remained at the sample
preparation levels. To automate this complex workflow microfluidics technology make
sense !
• The adoption of microfluidics solutions for sample preparation as already started in
molecular diagnostics, single cells and NGS assays.
• Sample prep problems solving through microfluidics devices will boost the microfluidic
technology exponentially by offering sample to answer results
Summary
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THANKYOU FORYOUR ATTENTION
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Time for Q&A
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available for sales
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and
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FromTechnologies to Market

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MEMS &
Sensors
LED
Compound
Semi.
Imaging Photonics
MedTech
Manufacturing
Advanced
Packaging
PV
Power
Electronics
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Yole Développement’s 30 analysts operate in the following areas
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