Line probe assay 26 7-15
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Line Probe Assay: A New Arrival at the PRL discusses a new diagnostic tool, the line probe assay, that has arrived at the Provincial Tuberculosis Reference Lab in Sindh, Pakistan. The line probe assay can simultaneously detect resistance to rifampicin and isoniazid in mycobacterium tuberculosis, with a sensitivity of over 90% and specificity of over 99% for smear-positive specimens. It provides results faster than conventional drug susceptibility testing methods like solid and liquid culture. The new diagnostic tool will help improve case detection and management of drug-resistant tuberculosis in Pakistan.
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Line probe assay 26 7-15
- 1. Line Probe Assay: A New Arrival at the PRL Dr. Muhammad Yahya Noori Assistant Professor Department of Pathology DIMC Focal Person for Provincial Tuberculosis Reference Lab Sindh DUHS
- 2. Tuberculosis Most common infectious cause of Mortality after HIV/AIDS 2
- 3. The Cost 2013 1.5 Million Deaths 9 Million New Cases Drug Sensitive TB $100-500/case MDR TB $9000-48000/case 3
- 7. Case Notification in Pakistan 7
- 8. Diagnosis of tuberculosis Latent Infection Active tuberculosis Smear examination Solid and liquid culture Identification Susceptibility testing methods TST IFN- techniques Molecular methods -Detection -Identification -Detection of resistance 8
- 9. Xpert MTB/RIF Assay Meta-Analysis Study • Average sensitivity for detection of rifampicin resistance: 94.1% • Average specificity for detection of rifampicin resistance: 97.0% • Average sensitivity in extra-pulmonary specimens: 80.4% • Average specificity in extra-pulmonary specimens : 86.1% 9
- 10. WHO Evaluation on Xpert MTB/RIF Assay-2011 • Xpert MTB/RIF assay: – Analytical sensitivity: 5 genome copies/purified DNA or 131 cfu/ml sputum (10-100 cfu/ml sputum for culture) 10
- 11. 11 Simultaneous Detection of Rifampicin and INH Resistance
- 12. 12
- 13. 13 Strip Reading
- 14. 14 For Smear Positive specimens: •Rifampicin: Sensitivity >97% Specificity >99% •In combination with INH: Sensitivity >90% Specificity >99% Sensitivity and Specificity
- 15. Thank you 15
Notas do Editor
- Prevalence >68K people suffering Incidence >55 K people falling ill every year..
- Around 3000