In this study, we developed a CRISPR/Cas9-based high throughput loss-of-function screen for identifying target genes responsible for the tumor proliferation and growth in TNBC. Our initial focus was to identify essential kinases in MDA-MB-231 cell line using the Invitrogen™ LentiArray™ Human Kinase CRISPR Library, which targets 840 kinases with up to 4 different gRNAs per protein kinase for complete gene knockout. This functional screen identified over 90 protein kinases that are essential for cell viability and cell proliferation. Ten of these hits (CDK1, CDK2, CDK8, CDK10, CDK11A, CDK19, CDK19, CDC7, EPHA2 and WEE1) are well-known targets validated in the literature. Currently, we are in the process validating the novel hits through target gene sequencing, western blotting and target specific small molecule kinase inhibitors.
The expression of ITPK in normal colon and colorectal cancer cells - Paper
Semelhante a Identifying novel and druggable targets in a triple negative breast cancer cell line using the Invitrogen™ LentiArray™ Human Kinase CRISPR Library
Semelhante a Identifying novel and druggable targets in a triple negative breast cancer cell line using the Invitrogen™ LentiArray™ Human Kinase CRISPR Library (20)