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SOIL BIOTECHNOLOGY/ SBT SYSTEM


All India Distillery Association, 26-27th February, 2011


              Dr. Avinash M Kadam (Ph.D, IIT Bombay)

                      Managing Director
              SUGAM PARYAVARAN VIKALP PVT LTD




                         www.sugam.in
What we do?

A.   Market & implement IIT Bombay patented technology
     “Soil Biofilter Technology” i.e. SBT

B.   Apply SBT for

       a. Sewage Treatment & Recycling

       b. Effluent Treatment & Recycling

       c. Grey Water Recycling

       d. Water Treatment & Recycling

                         www.sugam.in
What is SBT?

 Developed by IIT Bombay during 1988 – 2001
 Technology patented in USA and India

 Evergreen biological purification engine

 Process uses formulated media, culture & additive to achieve
  desired water quality




                          www.sugam.in
Technology Credentials
1.   Indigenously developed by IIT Bombay

2.   Covered under US & Indian Patent

3.   “Best innovation” award by Siemens –Water

4.   “One of the four best innovations” – Ministry of
     External Affairs, GOI

5.   Listed by Ministry of Defense for wastewater recycling

6.   Already in the domain of MCGM, KDMC etc.
WHY SBT????
Carbon cycle
                     CO2 in the
                    Atmosphere
6                       750                               45+45
        110


Photosynthesis              40-60      Respiration
                          (1-10 y)
                                                     90


        Plants                       Animals              CO2 4,000
         500                         100-150               Live 1-5
         (50 y)                        (2 y)             Dead carbon
                                                             750
                                                            (0.2y)
                  Dead Organics in
                        Soil   (1 y)
                                               50 - 65
             50                      18


    Fossil          Dead Organics                        Holdups 1015g per yr
    Fuels          Reserve in Soil                       Fluxes 1015g per yr
    4,000              1,576
                       (300 y)


             Global Carbon Cycle (Meilli, 1995)
ENERGY CONSUMPTION IN
 DIFFERENT HABITATS

 1. Water   500 kJ/g live C. yr

 2. Land        3   kJ/g live C.
yr
SCHEMATIC OF WATER RENOVATION

  Recycle (vr )            Feed (vf )



                   
                   
                           Reactor


                      C1
                                Discharge (vf )



                           C2
Typical Layout




  www.sugam.in
What is SBT System?


                                 Media
Culture                           Media
       e
Cultur


                      Soil
                     SBT
                 Biotechnology


                                          e
                                   Additiv
Plantation                       Additives
          tion
    Planta
USP
   It provides primary, secondary and tertiary treatment all in one
    unit; in a single evergreen facility open to atmosphere.
   It is very simple to operate, maintain.
   It does not require skilled man-power.
   It is cost competitive particular with reference to O&M cost.
   No pre-treatment
   No chemical usage
   Absence of any moving part
   Odour-free
   Low energy requirement and
   Green environment are its unique features.
What we can process?
1.Raw Water slurry
2. Air scrubbing
3. Leachates of solids
4. Waste water
    - Distillery spent leese/condensate treatment
    - Sugar Effluent
    - Ammoniacal-N containing Fertilizer Effluent
    - VOCs
    - Textile waste water
    - Dairy wastewater
    - Printing spent ink wash effluent Treatment
    - Industrial dye effluent treatment
    - Coffee Effluent treatment
SBT output water Quality




        www.sugam.in
AESTHATIC FOR WASTE PROCESSING??




             www.sugam.in
SBT PLANT




Renovation of colony sewage for irrigation in sports
Renovation of colony sewage for irrigation in sports
                     complex
                     complex
SBT PLANT




  Small Colony sewage
   Small Colony sewage
   treatment showing
    treatment showing
untreated & treated water
untreated & treated water
SBT PLANT




Retrofitting of idle activated sludge plant
Retrofitting of idle activated sludge plant
150 KLD at Naval Housing
Colony




      www.sugam.in
SBT PLANT




3MLD Sewage Purification in Corporation Of Bombay
Distribution




Operation Schematics
120 KLD Plant at M P. Airport, AAI, Udaipur, Rajasthan
REUSABLE WATER FROM WASTEWATER




  Wastewater      Treated Wastewater
1 MLD FACILITY FOR BPGC (Mumbai)
NOW WE INTRODUCE OUR SPECIALISED SOIL BIO TECHNOLOGY!!!
Our Sincere Acknowledgement to…




Rajarambapu Patil Sahakari Sakhar
         Karkhana Ltd
   Tal. Walva, Dist. Sangli, Maharashtra
DISTILLERY EVAPORATOR
CONDENSATE / SPENT LEESE
TREATMENT


pH   :   3–5
TDS :    100 mg/L
COD :    1500 – 3000 mg/L
BOD :    800 -1500 mg/L
ODOUR
VARIOUS TECHNOLOGICAL OPTION


- CONVENTIONAL AEROBIC TREATMENT

- CONVENTIONAL ANAEROBIC TREATMENT
- CONVENTIONAL AEROBIC TREATMENT FOLLOWED BY
MEMBRANE TECHNOLOGIES
- CONVENTIONAL ANAEROBIC TREATMENT FOLLOWED BY
MEMBRANE TECHNOLOGIES
- MEMBRANE TECHNOLOGIES ( RO – MBR) ALONE
An Exercise with Distillery Process
              Condensate recycling


• Location                   : Rajarambapu Patil SSK Ltd
                               Walva, Islampur,
                               Dist.Sangli, MS, INDIA

• Sugarcane Crushing :           12000 TCD/day
• Distillery production      :   100 cum/d
• Electricity export to grid:    7 MW/day
Plant summary

Item                                 Specification

Capacity.                            25 KL/day

Area                                 Existing Tank (100 sqm)

Duration of Operation.               12 hours per day

Rate of Sewage Input.                2.5 cum /hour

Output water                         24 kL

                                     Cooling make up and
Output Water Quality
                                     fermentation make up
Schematic of the process
       pH           Ozonation
Raw    adjustment
Tank                (10 ppm)
       with lime


                                               Settling
                                               (0.5 hr)




                                             Bioreactor
                                Recycling




                                            Discharge/
                                            Recycle
Schematic of the process
ETP or Garden??
ETP or Garden??
Output water Quality


                 COD (mg/L)               pH
Sr No
         Inlet        Outlet   Inlet       Outlet
 1          1750          27

 2          1350          34

 3          1450          30    3.0-4.5        7.2-8.0

 4          2400          40

 5          1800          32
Result means…….
Water Scenario at RBSSK:
• Water for Dilution with Molasses : 1000 cum/d
• Water for condensing, cooling,
  and blow down                       : 460 cum/d
• D.M. water requirement for dilution
  in New Plant                        : 190 cum/d
• Soft water requirement for Vacuum
  pump, Alcohol scrubber and F.O.
  Decanter                             : 190 cum/d
• D.M. water requirement for dilution
  in ENA plant                         : 120 cum/d
                               TOTAL: 1960 cum/d
Result means…….

Water for recycling: >720 cum/d (out of 750 cum/d)
                       SBT Output water




   Disinfection
                                          Softener



   Water for
   Dilution in                            Water for
   Fermentor                              cooling
Dr. Avinash M. Kadam            Mr. Kiran Kotian
Ph.D (Chem Engg., IIT Bombay)   (Head Projects)
sugamparyavaran@gmail.com       Email: kiran@sugam.in
Phone: +91 9769838718           Phone: +91 9833663346
       +91 9833663346
                          www.sugam.in
We do ……………..
• Design and Turnkey execution of SBT and SBT plants for
  STP and ETP applications
• Retrofit of pre-existing STP/ETPs with SBT systems
• Design of zero discharge air scrubbers for removal of waste
  noxious gases (Sulphur, NOx and organic pollutants from
  Industrial processes).
• Design Consultancy for Water and Waste Streams Auditing,
  targeting Zero Discharge Norms
• Energy Auditing and Pinch analysis of heat sources to
  reduce energy consumption.
• Distillery Process Condensate Treatment
• Printing spent ink wash effluent Treatment
• Industrial dye effluent treatment
• Coffee Effluent treatment
                        www.sugam.in
PROCESS CHEMISTRY
Aerobic Respiration
(CH2ONxPySzKy)n + nO2 + nH2O = nCO2 + 2nH2O + Mineral (N, P, S, K) + Energy                     (1)

Photosynthesis
nCO2 + 2nH2O + Minerals (N,P, S,K) + Sunlight = [CH2ONxPySzKy]n + nO2 + nH2O (Photosynthesis)    (2)
Nitrogen Fixation
N2 + 2H2O + Energy = NH3 + O2 ( in soil)                                                         (3)
N2 + 2H2O + Light = NH3 + O2 (in water)                                                          (4)

Acidogenesis
4C3H7O2NS (cell mass) + 8H20 = 4CH3COOH + 4CO2 + 4NH3 + 4H2S + 8H+ + 8e-                        (5)
Methanogenesis
8H+ + 8e- + 3CH3COOH + CO2 = 4CH4 + 3CO2 + 2H2O                                                  (6)
    Adding 5 and 6 give overall biomethanation chemistry
4C3H7O2NS + 6H20 = CH3COOH + 6CO2 + 4CH4 + 4NH3 + 4H2S                                          (7)

Mineral weathering
CO2 + H2O = HCO3 - + H+                                                                          (8)
Primary mineral + CO2 + H2O = M+n + n HCO3 - + soil/clay/sand                                     (9)

Nitrification
NH3 + CO2 + 1.5O2 = Nitrosomonas + NO2- + H2O + H+                                               (10)
NO2-+ CO2 + 0.5O2 = Nitrobacter + NO3-                                                           (11)


Denitrification
4NO3- + 2H2O + energy = 2N2 + 5O2 + 4OH -                                                       (12a)
Components of CSF Media
                                              Influent Waste
                                                   Water
           Media: Respiration                                               Plantation:
                                                                          Photosynthesis
      (CH2ONxPySzKy)n+nO2+nH2O =
     nCO2+2n H2O+ Mineral + Energy                                  nCO2+ 2n H2O+ (N,P,S,K) +
          NH4+NO2 = N2+H2O                                          Sunlight= [CH2ONxPySzKy]n+
    4C3H7O2NS + 8H20 = 4CH3COOH +                                            nO2+ H2O
             4CO2 + 4NH3
   3CH3COOH + CO2 = 4CH4 + 3CO2 +
                 2H2O




                                          Constructed Soil Filter
N2+H2O =                                      (CSF) Media                                        Natural
                                              Formulation                                          O2
 NH3+O2




           Additives: Weathering                                         Microorganisms
                                                                           (Task Force)
    CO2 + H2O = HCO3 - + H+                                                     +
                                                                         Geophagus worm
    Primary mineral + CO2 + H2O = M+n +                                 (Controls ecology)
          nHCO3 -+ Soil/clay/sand




                                             Clear Effluent
Why Weathered Environment ?
 Microbial Diversity (Curtis & Sloan, Science 309, 2005)

   Soil            : 1012-1016 cells/g soil; 105-106 genera         Oceans: 160 per mL;

   Water           : 103 genera                                     Soil: 6400-38000 per g;
                                                                    Sewage: 70 per mL

 Energetic (ca.)

   Land            : 3.2 kJ/g.live C.yr
   Water           : 700 kJ/g.live C.yr


 Oxygen diffusion coefficient (Perry’s Handbook, 1999)

   Soil             : 10-5 m2s-1
   Water            : 10-9 m2s-1
   Solubility of Oxygen in water is very low (0.3 millimol/L at 20 0C)
 Immobilization of pollutant; hence high reaction rates Vs local pollution becomes global;
  low reaction rates due to dilution
 Heterogenous system; housing multiple microenvironment viz. oxic, anoxic and
  anaerobic within same niche Vs only one within the same niche..
PERFORMANCE VARIATION OF SBT PLANT                                   :
       20                                                                            20


       18                    COD     SS    DO     BOD       Ammonical-N   pH         18


       16                                                                            16


       14                                                                            14


       12                                                                            12
                                                                                         pH
mg/l




       10                                                                            10


        8                                                                            8


        6                                                                            6


        4                                                                            4


        2                                                                            2


        0                                                                            0
              June   July   August    September   October      November   December


                               www.sugam.in
Layout for processing of Wastewater




             www.sugam.in
Media & Culture
•   Underdrain:- Stone rubble of various sizes ranging upto Gravel (200.0-2.0
    mm), Very coarse sand (1.0-2.0 mm), Coarse sand (0.5-1.0 mm), Medium
    sand (0.25-0.5 mm), Fine sand (0.1-0.25 mm)


•   Media:- Formulated from soil as required and primary minerals of suitable
    particle size and composition


•   Culture:- Geophagus (Soil living) worm Pheretima elongata and bacterial
    culture from natural sources containing bacteria capable of processing
    cellulose, lignin, starch, protein, also nitrifying and denitrifying organisms.
    Anaerobic organisms for methanogenesis. For industrial wastes,
    development of appropriate culture required


•   Additives:- Formulated from natural materials of suitable particle size and
    composition to provide sites for respiration, CO2 capture


•   Bioindicators:- Green plants particularly with tap root system

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Soil bio technology

  • 1. SOIL BIOTECHNOLOGY/ SBT SYSTEM All India Distillery Association, 26-27th February, 2011 Dr. Avinash M Kadam (Ph.D, IIT Bombay) Managing Director SUGAM PARYAVARAN VIKALP PVT LTD www.sugam.in
  • 2. What we do? A. Market & implement IIT Bombay patented technology “Soil Biofilter Technology” i.e. SBT B. Apply SBT for a. Sewage Treatment & Recycling b. Effluent Treatment & Recycling c. Grey Water Recycling d. Water Treatment & Recycling www.sugam.in
  • 3. What is SBT?  Developed by IIT Bombay during 1988 – 2001  Technology patented in USA and India  Evergreen biological purification engine  Process uses formulated media, culture & additive to achieve desired water quality www.sugam.in
  • 4. Technology Credentials 1. Indigenously developed by IIT Bombay 2. Covered under US & Indian Patent 3. “Best innovation” award by Siemens –Water 4. “One of the four best innovations” – Ministry of External Affairs, GOI 5. Listed by Ministry of Defense for wastewater recycling 6. Already in the domain of MCGM, KDMC etc.
  • 6. Carbon cycle CO2 in the Atmosphere 6 750 45+45 110 Photosynthesis 40-60 Respiration (1-10 y) 90 Plants Animals CO2 4,000 500 100-150 Live 1-5 (50 y) (2 y) Dead carbon 750 (0.2y) Dead Organics in Soil (1 y) 50 - 65 50 18 Fossil Dead Organics Holdups 1015g per yr Fuels Reserve in Soil Fluxes 1015g per yr 4,000 1,576 (300 y) Global Carbon Cycle (Meilli, 1995)
  • 7. ENERGY CONSUMPTION IN DIFFERENT HABITATS 1. Water 500 kJ/g live C. yr 2. Land 3 kJ/g live C. yr
  • 8. SCHEMATIC OF WATER RENOVATION Recycle (vr ) Feed (vf )     Reactor C1 Discharge (vf ) C2
  • 9. Typical Layout www.sugam.in
  • 10. What is SBT System? Media Culture Media e Cultur Soil SBT Biotechnology e Additiv Plantation Additives tion Planta
  • 11. USP  It provides primary, secondary and tertiary treatment all in one unit; in a single evergreen facility open to atmosphere.  It is very simple to operate, maintain.  It does not require skilled man-power.  It is cost competitive particular with reference to O&M cost.  No pre-treatment  No chemical usage  Absence of any moving part  Odour-free  Low energy requirement and  Green environment are its unique features.
  • 12. What we can process? 1.Raw Water slurry 2. Air scrubbing 3. Leachates of solids 4. Waste water - Distillery spent leese/condensate treatment - Sugar Effluent - Ammoniacal-N containing Fertilizer Effluent - VOCs - Textile waste water - Dairy wastewater - Printing spent ink wash effluent Treatment - Industrial dye effluent treatment - Coffee Effluent treatment
  • 13. SBT output water Quality www.sugam.in
  • 14. AESTHATIC FOR WASTE PROCESSING?? www.sugam.in
  • 15. SBT PLANT Renovation of colony sewage for irrigation in sports Renovation of colony sewage for irrigation in sports complex complex
  • 16. SBT PLANT Small Colony sewage Small Colony sewage treatment showing treatment showing untreated & treated water untreated & treated water
  • 17. SBT PLANT Retrofitting of idle activated sludge plant Retrofitting of idle activated sludge plant
  • 18. 150 KLD at Naval Housing Colony www.sugam.in
  • 19. SBT PLANT 3MLD Sewage Purification in Corporation Of Bombay
  • 21.
  • 22. 120 KLD Plant at M P. Airport, AAI, Udaipur, Rajasthan
  • 23. REUSABLE WATER FROM WASTEWATER Wastewater Treated Wastewater
  • 24. 1 MLD FACILITY FOR BPGC (Mumbai)
  • 25. NOW WE INTRODUCE OUR SPECIALISED SOIL BIO TECHNOLOGY!!!
  • 26. Our Sincere Acknowledgement to… Rajarambapu Patil Sahakari Sakhar Karkhana Ltd Tal. Walva, Dist. Sangli, Maharashtra
  • 27. DISTILLERY EVAPORATOR CONDENSATE / SPENT LEESE TREATMENT pH : 3–5 TDS : 100 mg/L COD : 1500 – 3000 mg/L BOD : 800 -1500 mg/L ODOUR
  • 28. VARIOUS TECHNOLOGICAL OPTION - CONVENTIONAL AEROBIC TREATMENT - CONVENTIONAL ANAEROBIC TREATMENT - CONVENTIONAL AEROBIC TREATMENT FOLLOWED BY MEMBRANE TECHNOLOGIES - CONVENTIONAL ANAEROBIC TREATMENT FOLLOWED BY MEMBRANE TECHNOLOGIES - MEMBRANE TECHNOLOGIES ( RO – MBR) ALONE
  • 29. An Exercise with Distillery Process Condensate recycling • Location : Rajarambapu Patil SSK Ltd Walva, Islampur, Dist.Sangli, MS, INDIA • Sugarcane Crushing : 12000 TCD/day • Distillery production : 100 cum/d • Electricity export to grid: 7 MW/day
  • 30. Plant summary Item Specification Capacity. 25 KL/day Area Existing Tank (100 sqm) Duration of Operation. 12 hours per day Rate of Sewage Input. 2.5 cum /hour Output water 24 kL Cooling make up and Output Water Quality fermentation make up
  • 31. Schematic of the process pH Ozonation Raw adjustment Tank (10 ppm) with lime Settling (0.5 hr) Bioreactor Recycling Discharge/ Recycle
  • 32. Schematic of the process
  • 35. Output water Quality COD (mg/L) pH Sr No Inlet Outlet Inlet Outlet 1 1750 27 2 1350 34 3 1450 30 3.0-4.5 7.2-8.0 4 2400 40 5 1800 32
  • 36. Result means……. Water Scenario at RBSSK: • Water for Dilution with Molasses : 1000 cum/d • Water for condensing, cooling, and blow down : 460 cum/d • D.M. water requirement for dilution in New Plant : 190 cum/d • Soft water requirement for Vacuum pump, Alcohol scrubber and F.O. Decanter : 190 cum/d • D.M. water requirement for dilution in ENA plant : 120 cum/d TOTAL: 1960 cum/d
  • 37. Result means……. Water for recycling: >720 cum/d (out of 750 cum/d) SBT Output water Disinfection Softener Water for Dilution in Water for Fermentor cooling
  • 38. Dr. Avinash M. Kadam Mr. Kiran Kotian Ph.D (Chem Engg., IIT Bombay) (Head Projects) sugamparyavaran@gmail.com Email: kiran@sugam.in Phone: +91 9769838718 Phone: +91 9833663346 +91 9833663346 www.sugam.in
  • 39. We do …………….. • Design and Turnkey execution of SBT and SBT plants for STP and ETP applications • Retrofit of pre-existing STP/ETPs with SBT systems • Design of zero discharge air scrubbers for removal of waste noxious gases (Sulphur, NOx and organic pollutants from Industrial processes). • Design Consultancy for Water and Waste Streams Auditing, targeting Zero Discharge Norms • Energy Auditing and Pinch analysis of heat sources to reduce energy consumption. • Distillery Process Condensate Treatment • Printing spent ink wash effluent Treatment • Industrial dye effluent treatment • Coffee Effluent treatment www.sugam.in
  • 40. PROCESS CHEMISTRY Aerobic Respiration (CH2ONxPySzKy)n + nO2 + nH2O = nCO2 + 2nH2O + Mineral (N, P, S, K) + Energy (1) Photosynthesis nCO2 + 2nH2O + Minerals (N,P, S,K) + Sunlight = [CH2ONxPySzKy]n + nO2 + nH2O (Photosynthesis) (2) Nitrogen Fixation N2 + 2H2O + Energy = NH3 + O2 ( in soil) (3) N2 + 2H2O + Light = NH3 + O2 (in water) (4) Acidogenesis 4C3H7O2NS (cell mass) + 8H20 = 4CH3COOH + 4CO2 + 4NH3 + 4H2S + 8H+ + 8e- (5) Methanogenesis 8H+ + 8e- + 3CH3COOH + CO2 = 4CH4 + 3CO2 + 2H2O (6) Adding 5 and 6 give overall biomethanation chemistry 4C3H7O2NS + 6H20 = CH3COOH + 6CO2 + 4CH4 + 4NH3 + 4H2S (7) Mineral weathering CO2 + H2O = HCO3 - + H+ (8) Primary mineral + CO2 + H2O = M+n + n HCO3 - + soil/clay/sand (9) Nitrification NH3 + CO2 + 1.5O2 = Nitrosomonas + NO2- + H2O + H+ (10) NO2-+ CO2 + 0.5O2 = Nitrobacter + NO3- (11) Denitrification 4NO3- + 2H2O + energy = 2N2 + 5O2 + 4OH - (12a)
  • 41. Components of CSF Media Influent Waste Water Media: Respiration Plantation: Photosynthesis (CH2ONxPySzKy)n+nO2+nH2O = nCO2+2n H2O+ Mineral + Energy nCO2+ 2n H2O+ (N,P,S,K) + NH4+NO2 = N2+H2O Sunlight= [CH2ONxPySzKy]n+ 4C3H7O2NS + 8H20 = 4CH3COOH + nO2+ H2O 4CO2 + 4NH3 3CH3COOH + CO2 = 4CH4 + 3CO2 + 2H2O Constructed Soil Filter N2+H2O = (CSF) Media Natural Formulation O2 NH3+O2 Additives: Weathering Microorganisms (Task Force) CO2 + H2O = HCO3 - + H+ + Geophagus worm Primary mineral + CO2 + H2O = M+n + (Controls ecology) nHCO3 -+ Soil/clay/sand Clear Effluent
  • 42. Why Weathered Environment ?  Microbial Diversity (Curtis & Sloan, Science 309, 2005) Soil : 1012-1016 cells/g soil; 105-106 genera Oceans: 160 per mL; Water : 103 genera Soil: 6400-38000 per g; Sewage: 70 per mL  Energetic (ca.) Land : 3.2 kJ/g.live C.yr Water : 700 kJ/g.live C.yr  Oxygen diffusion coefficient (Perry’s Handbook, 1999) Soil : 10-5 m2s-1 Water : 10-9 m2s-1 Solubility of Oxygen in water is very low (0.3 millimol/L at 20 0C)  Immobilization of pollutant; hence high reaction rates Vs local pollution becomes global; low reaction rates due to dilution  Heterogenous system; housing multiple microenvironment viz. oxic, anoxic and anaerobic within same niche Vs only one within the same niche..
  • 43. PERFORMANCE VARIATION OF SBT PLANT : 20 20 18 COD SS DO BOD Ammonical-N pH 18 16 16 14 14 12 12 pH mg/l 10 10 8 8 6 6 4 4 2 2 0 0 June July August September October November December www.sugam.in
  • 44. Layout for processing of Wastewater www.sugam.in
  • 45. Media & Culture • Underdrain:- Stone rubble of various sizes ranging upto Gravel (200.0-2.0 mm), Very coarse sand (1.0-2.0 mm), Coarse sand (0.5-1.0 mm), Medium sand (0.25-0.5 mm), Fine sand (0.1-0.25 mm) • Media:- Formulated from soil as required and primary minerals of suitable particle size and composition • Culture:- Geophagus (Soil living) worm Pheretima elongata and bacterial culture from natural sources containing bacteria capable of processing cellulose, lignin, starch, protein, also nitrifying and denitrifying organisms. Anaerobic organisms for methanogenesis. For industrial wastes, development of appropriate culture required • Additives:- Formulated from natural materials of suitable particle size and composition to provide sites for respiration, CO2 capture • Bioindicators:- Green plants particularly with tap root system

Notas do Editor

  1. 1 MLD
  2. Prakriti Eco-Engineering Associates
  3. Respiration : 400-800 mv Photosynthesis = 100-300 mv Nitrogen Fixation = 100-300 mv Acidogenesis = 100-600 mv Methanogenesis = -100- +100 mv Mineral Weathering Nitrification = 600-800 Anamox = 100-300 Denitrification = 100-300