This document discusses stem cell therapy and its potential to treat various incurable or "no hope" disorders. It provides an overview of stem cells, their sources and types. Mesenchymal stem cells from sources like bone marrow, umbilical cord blood and adipose tissue are highlighted as having advantages for therapy due to their ability to differentiate, lack of ethical issues, and potential to be used across HLA barriers. A number of incurable conditions are identified where stem cell therapy may be effective or very effective, including diabetes, neurological disorders, anti-aging, and orthopedic issues. The success of stem cell therapy depends on isolating and culturing the appropriate stem cell types for differentiation. Questions about the therapy are also addressed

1. Dr. Sharda Jain
Dr. Ajay saxena
Dr. Rajesh
Dhall
:A Hope to No Hope Disorders
Stem Cell Therapy

2. Life is too Precious -
don’t give up !!
Mother Teresa

3. STEM CELL Therapy
A HOPE TO NO HOPE DISORDERS

4. The fascinating new world of
STEM CELL THERAPY :
a medical revolution at the corner
Welcome
to

5. Future of Stem Cell Medicine
• The Indian market potential slated to be in the range of US$ 500 million.
• More than 45,000 people across the world receiving stem cell transplant
every year.
• Potential to reduce pharmaceutical R&D by 25% each year, with savings of
up to US $25 million in each drug market.
• Potential to be the next revolution in the field of medicine.
LifeCare – ReeCure Centre
of STEM CELL Therapy

6. “The Need”
• The physical misery
• Psychological trauma
Of “No Hope Disorder” is great!!
• No cure available for most of these diseases

7. Rationale of is stem cell
Therapy ??
Introduction of adult stem cells into
damaged tissue in order to treat
injuries / Disease in
“No Hope Disorders”

8. Stem cell potential
… beating cardiac muscle

9. • Is it really working?
• Why not studies are coming from premier institutes of
country ?
• Is it really possible to segregate Mesenchymal cells?
• Differentiation of cells out side body possible in India???
• If it is too good then, why not NOBEL PRIZE given ?
• ?
• ??
• ???
• Is this therapy available in India?
Questions ????

10. Times
of
India
9th
October 2012

11. We are sure there are a lot of doubts plaguing
your mind about STEM CELL BANKING and
STEM CELL THERAPY.
Let us help you to ERASE these doubts.
Doubts… on SCT

12. 1913 the great Spanish neuroscientist Santiago Ramón y Cajal
pronounced “that in adult Neurone & the nerve paths –
Everything may die, nothing may be regenerated”.
Nerve Cell
Cannot
Regenerate ?

13. Heart Brain
Same is our perception
for any tissue !!

14. Beliefs turned out to be FALSE as
several types of cells can regenerate

15. Major Milestones
Which Triggered Adult Stem Cell
Research ?
• Since1970’s,
BONE MARROW TRANSPLANTS
have been used for treatment of
Immunodeficient &
leukemia (with Good results).

16. 2nd
Breakthrough…
History of Human Embryonic
Stem Cell Research
In 1998, James Thomson (University of
Wisconsin-Madison) isolated cells from the inner
cell mass of the blastocyst, and developed the
first human embryonic stem cell line in culture.

17. Prediction was made of Making cells
and replacing all the diseased cells?
& even gametes

18. Stem Cell Potential

19. Stem Cell History
• 1970 – BM transplant for leukaemia
• 1998 - Researchers first extract stem cells from human
embryos
• 1999 - First Successful human transplant of insulin-making cells
from cadavers
• 2001 - President Bush restricts federal funding for embryonic
stem-cell research
• 2002 - Juvenile Diabetes Research Foundation International
creates $20 million fund-raising effort to support stem-
cell research
• 2003?? - California ok stem cell research
• 2004 - Harvard researchers grow stem cells from embryos
using private funding
• 2004 - Ballot measure for $3 Billion bond for stem cells
• 2006-7 – Private Banking for cord & cord blood in india.
• 2014 – Public cord banking Kolkatta – Inst. Tropical med.

21. What are Stem Cells
• Ability to differentiate
• Ability to regenerate

22. Stem Cell is a
•young,
•primitive and unspecialized cell with a
remarkable potential to renew,
•Differentiate and
Develop into any desired tissue or organ of
the body.
What is stem cells ?

23. Self renewal: Unlimited proliferative potential.
Differentiation: Differentiate into various cell types
Pluripotency,
Multipotency,
Unipotency)
Plasticity : Trans-differentiation
Regeneration potential: a means of Repair
Characteristic Features

24. A single
precursor
What is Differentiation?

25. Cells having infinite self renewing capacity and
potential for differentiation.
What are Stem Cells?

26. 1000 IEC ICAs in
biocompatible capsule IP Tx
STZ Diabetic mice
Blood glucose analysis of STZ induced diabetic
mice transplanted with hUCMSCs and Islet like
clusters (ILC)
0 10 20 30
0
100
200
300
400
500
hUCMSCs
ILCs
Normal
Diabetic control
Days after transplantationBloodglucoseconcentration
mg/dl
0 25 50 75 100 125 150
0
100
200
300
400
ILC
diabetic control
non diabetic control
time (mins)
Bloodglucoseconcentration
mg/dl
Glucose
Tolerance
Test
Fasting blood glucose concentration of diabetic mice after transplantation of ILCs showed
that the hyperglycemic mice reverted back to normoglycemia. Intraperitoneal glucose
tolerance test was performed on 45th
day, post-transplantation. Mice transplanted with ILCs
showed similar blood glucose profile as compared to the normal non diabetic controls.

27. How MSCs work In vivo
Under normal circumstances MSC contribute to tissue maintenance, contributing to
immune homeostasis by avoiding unnecessary immune cell response.
With damaged tissue,
harmonious interaction between
MSC and endothelial cells are
disrupted, allowing blood
extravasations , blood clot form,
platelet release multitude of
bioactive molecule, immune
cells are attracted where they
start immune surveillance and
immune response.
MSC secrete bioactive molecules that
exert anti-apoptotic, immunomodulatory ,
angiogenic , anti-scaring , anti-fibrotic
and chemoattractant effects

28. Source of Adult Stem cells
• Autologus,
• Allogeneic or
• Xenogenic
Fetal tissue is the best current tissue source for
human stem cells,
However ethical issues are a major concern.
(ICMR – Autologous)

29. The embryos of
aborted fetuses
Umbilical cord
and cord blood of a new
born baby
Bone Marrow
Peripheral
Bloodstream
Adult Tissues Amniotic Fluid
MENSTRUAL BLOOD Dental Pulp
Sources of Stem Cells
Hair

30. Best Source
Amniotic fluid,
umbilical cord,
Cord blood -
Rich Source of
Stem Cells

31. Cord Blood Stem Cells
• Can be used only
to cure chronic
blood-related
disorders such as
sickle cell
disease,
Thalasemia, and
leukaemia.

32. Umbilical Cord Blood Stem Cell
Transplant
• Umbilical cord blood
stem cell transplants are
less prone to
rejection than either
bone marrow or
peripheral blood stem
cells. This is probably
because the cells have
not yet developed the
features that can be
recognized and attacked
by the recipient's
immune system

33. Placental Blood as a Source of Hematopoietic
Stem Cells for Transplantation into
UNRELATED RECIPIENTS
Joanne Kurtzberg, M.D.et al

34. PLASTICITY: The ability of one tissue to produce cells of other developmentally
unrelated tissues
Traditionally, stem cells were believed to be Lineage Restricted and
Organ Specific.
Bone marrow stem cells:
The gold standard

35. AUTOLOGUS – STEM CELLS
• While most blood stem
cells reside in the bone
marrow, a small number
are present in the
bloodstream. These
multipotent peripheral
blood stem cells, or
PBSCs, can be used
just like bone marrow
stem cells to treat
leukaemia, other
cancers and various
blood disorders

36. Adult Stem Cells - Hair

37. Dental Pulp
Menstrual Blood

38. Allogeneic – Stem Cells
• Sources of stem cells
from another donor
(allogeneic) are
primarily relatives
(familial- allogeneic) or
completely unrelated
donors (unrelated-
allogeneic). The stem
cells in this situation
are extracted from
either the donor's body
or cord blood

39. Xenogeny - Stem Cells
• In this stem cells from
different species are
transplanted, e.g. porcine
fetal ventral mesencephalic
(FVM) xenotransplants for
Parkinson's disease.
This has no major ethical
concerns and a large amount
of tissue is available, however
life long immunosupression
and risk of rejection are the
major limitations

40. Prediction for Future
Cells from these Animal will
be used in human beings
Without risk of rejection

41. THE SUCCESS OF STEM CELL
THERAPY DEPENDS ON LAB.

42. These are
• Mono Nuclear cells ( MNC )
• Hematopoietic Stem Cells [HSC] family:
CD34+; CD45; CD133
• Mesenchymel count in M.N.C is just 0.001%
• Mesenchymel stem cell [MSC]: CD73; CD90;
CD106 Etc ..........
Classification Of Stem Cells

43. Differentiation with different cluster
coding/desire cells type ( as per disorder)
through culture.. (Mesenchymal types are -
CD 73, CD 90, CD 105 etc . .. . .
• Multiplication up label of required cells
quantity.( near to 1.5 Cr.).
• Composition of all cells like Mnc, Hsc & Msc.
Technology Behind Success

44. Bone Marrow Menstrual BloodHair Follicle Adipose
Tissue
STEM CELL THERAPY

45. Homogeneous
population
and high rate
of cell division
Homogeneous
population
and high rate
of cell division
No risk of
rejection –
USED across
HLA barrier
No risk of
rejection –
USED across
HLA barrier
No ethical
issues
No ethical
issues
Immune
privileged
Immune
privileged
Greater
potency of
cultured
expanded
product
Greater
potency of
cultured
expanded
product
Precise
identification
Precise
identification
Ease of
isolation and
scale up
Ease of
isolation and
scale up
Genetic
stability
Genetic
stability
Advantages ofAdvantages of
MesenchymalMesenchymal
Stem CellsStem Cells
No risk of
teratoma
formation
No risk of
teratoma
formation
PluripotentPluripotent
Efficient large
scale
expansion
Efficient large
scale
expansion
Lower cost of
cell culture
process
Lower cost of
cell culture
process
Why MSCs ?

46. Indications

47. Indications
STEM CELL Therapy

48. • Diabetes
• Cerebral Palsy
• Multiple Sclerosis
• Parkinson’s Disease
•Alzheimer’s Disease
• Autism
• Anti-Ageing
Very Effective

49. Diabetes
STEM CELL THERAPY FOR DIABETES

50. Neuro-Muscular
Disorders
• Cerebral Palsy
√
• Multiple Sclerosis
√
• Parkinson’s Disease
√
• Autism √
• Spinal Cord Injury
• Stroke
•Alzheimer’s Disease
• Muscular Dystrophy
STEM CELL Therapy for
Neuro – Muscular Disorders

51. STEM CELL THERAPY FOR
Anti-Ageing

52. Effective
• Liver Cirrhosis
• Non-healing fractures
• Stroke • Osteogenesis Imperfecta
• Muscular Dystrophy • Myocardial Infarction
• Spinal Muscular Atrophy • Cardiomyopathy
• ALS • Chronic Heart Failure
• Osteoarthritis • Osteogenesis Imperfecta
• Non-healing fractures

53. • Osteoarthritis
• Non-healing fractures
• Osteogenesis Imperfecta
• Achondrodysplasia
Bone and Cartilage
Disorders
Cardiovascular Disorders
• Chronic Heart Failure
• Myocardial Infarction
• Cardiomyopathy
• Buerger’s Disease
STEM CELL Therapy for
Bone and Cartilage Disorders

54. KIDNEY LIVER
STEM CELL THERAPY FOR
KIDNEY /LIVER DISORDER

55. Least Effective
• Spinal Cord Injury
• Buerger’s Disease
• Eye Disorder

56. Not Effective/ Need More Trails
Acute Leukaemia • Inherited Platelet Abnormalities
• Chronic Leukaemia • Other Inherited Disorder
• Myelodysplastic Syndromes • Inherited Metabolic Disorders
• Marrow Failure • Histiocytic Disorders
• Thalassemia • Inherited Erythrocyte Abnormalities
• Myeloproliferative Disorders • Inherited Immune System Disorders
• Lymphoproliferative Disorders • Plasma Cell Disorders
• Phagocyte Disorders • Other Malignancies

57. Drug Testing - Quicker
• Stem cells could allow scientists to test
new drugs using human cell line which
could speed up new drug development.
• Only drugs that were safe and had
beneficial effects in cell line testing would
graduate to whole animal or human
testing.
• It would allow quicker and safer
development of new drugs.

58. Let’s
Create a better tomorrow
for
Spread the message
Please create Public Awareness
Spinal injury
My cord blood treated
Bhai’s thalassemia major Cured of insulin dep. DM
Super man
Ajit Jogi

59. Only company in India with 9 type of stem cell
banking and treatment through Reecure
50 centres spread across India
Mr. Ajay Saxena
Ph. 8467888005
Website : www.reelabs.com
Email : ajay.saxena@reelabs.com
Q.& A.session Dr. Sharda Jain
Mr. Ajay Saxena

60. STEM CELL
Therapy
A HOPE TO NO
HOPE DISORDERS
Thank you