The document proposes a project to produce plant protein by cultivating spirulina in Kaimur District, Bihar, India over 300 days. It details the health benefits of spirulina, requirements for growing spirulina commercially, and provides estimates of the setup costs, operating costs, expected output, and projected profits of establishing a 500 square meter spirulina farm. The project aims to fight malnutrition, enhance food security, and generate employment in India by producing an affordable and nutrient-rich protein source.

1. PRODUCTION OF PLANT
PROTEIN USING
SPIRULINA
SUBMITTED TO :
DR. KALYAN GHADEI
DEPARTMENT OF
EXTENSION
EDUCATION
SUBMITTED BY :
SHASHI
SHEKHAR
B.SC. (AG) PART
IV
ID NO. 14140

2. o For adults the protein requirement per kg body weight is considered
to be the same for both sexes at all ages and body weights within the
acceptable range. The value accepted for the safe level of intake is
0.75 g per kg per day, in terms of proteins with the digestibility of milk
or egg.
o This amounts to:
• 56 grams per day for the average sedentary man.
• 46 grams per day for the average sedentary woman.
o One kg of dry spirulina powder is being sold at Rs. 1,000 in the

3. o Lower Risk of Heart Disease
o Reduced Risk of Type 2 Diabetes
o Protection Against Weight Gain
o Correlation Does Not Imply Causation

4. o India has ranked 100 among 119 developing countries as per report titled “ 2017
global hunger Index : The Inequalities of hunger”
o With score of 31.4, India is at the high end of “serious” hunger problem category
and one of the main factors pushing South Asia to the category of the worst-
performing region.
o India has seen low improvement in hunger prevalence despite being world’s
second largest food producer, and world’s fastest growing economy.
o India’s poor performance shows country’s stubbornly high proportions of
malnourished children – with about 21 percent Indian children under five suffer
from wasting and 38.4 percent from stunting.

5. 1.To develop innovative, cost-effective and resource-
efficient food
2.To significantly enhance the quality and quantity of
proteins
3.New age employment generation
4.To fight against malnutrition particularly in child and
women
5.To enhance food security in India

6. Our mission is to work with National Nutrition Mission’s target i.e., NNM targets
to reduce stunting, under-nutrition, anemia (among young children, women and
adolescent girls) and reduce low birth weight by 2%, 2%, 3% and 2% per annum
respectively.

7. Name of Project : Production of Plant Protein using Spirulina
Duration of the Project : 300 days
Timeline to start the project : Feb 2019 to Nov 2019
Area Required : 100 * 5 sq metres
Geography of Proposed Project : Kaimur District, Bihar, India
About Kaimur :
Location – 84 E longitude and 26 N
latitude in central based border of Bihar
Average temperature – 30 degree
Celsius
Climate – Tropical climate
Area – 8, 268 sq. km

8. Spirulina is a type of naturally occurring blue-green
micro-algae which can be grown in warm water
alkaline water conditions. Spirulina can be grown in
wild waters and at home/indoors.
As algae can absorb toxins from the environment,
most of the people showing interest to grow Spirulina
indoors or at home under controlled conditions.

9. o Spirulina used in the manufacture of several medicines and
cosmetics, The following are some of the health benefits of
Spirulina.
o Spirulina Strengthens the immune system.
o It improves the digestion.
o Spirulina may prevent from certain type of cancers.
o It is good for women and children since it contains easy to absorb
iron supplements.
o It is excellent source of beta carotene hence can consider this as
good anti-oxidant and good for eye health Good for muscle building

10. o The Immune System Miracle Worker (San Francisco Medical Research Foundation)
o Worlds’ Most Powerful Food (biotech-firm.com)
o Spirulina is the most ideal food for mankind (United Nations Recommendation)
Spirulina: Food for the future (Publication by the USDA – United States Department of
Agriculture).
o “There is a need for both national governments and inter-governmental organizations to
re-evaluate the potential of Spirulina to fulfil both their own food security needs as well
as a tool for their overseas development emergency response efforts” – The UN-Food
and Agriculture Organisation (FAO) Report on Spirulina 2008.
o “For WHO, Spirulina represents an interesting food for multiple reasons, rich in
iron and protein, and is able to be administered to children without any risk. We at
WHO consider it a very suitable food.” – United Nations World Health Organization
(WHO), Geneva, Switzerland June 8Th, 1993

11. o You must have a tank with your preferable size. It can be a cement or plastic.
o In order to harvest and squeeze the water out of thick Spriluna, you must have some kind of
harvesting equipment like fine cloth and mesh.
o You may require Thermometer, air pump (for agitation), water pump for circulation, pH meter,
Electric water heater, and any LED lights.
o Plain water may not be good option for growing Spirulina, so you need to buy minerals for
better growth of algal. This food or cultural medium is mandatory for growing this super food.
o Plain filtered water plus Sodium bicarbonate plus Magnesium sulfate plus Potassium nitrade
plus Citric acid plus Common salt plus Urea plus Calcium chloride plus Iron sulfate plus
Ammonium sulfate make the perfect cultural medium.

12. o Spirulina thrives best in warm temperatures and ideal temperatures for its growth is between 25 and
38 degrees Celsius. Though this can tolerate low temperatures, it is always better to provide warm
climatic conditions. You can use water heaters to increase the required temperature.
o You need Spirulina seeds to start your culture or you can buy Spirulina starter kit in any organic store.
Spirulina starter kit contains the mother Spirulina culture (algae) in the bottle along with medium
(water). You must make sure to buy from certified store.
o Though it can be grown in any size of tank, the preferable tank size is about 10 feet x 5 feet x 1.5
feet. Fill this tank with 1000 litres of water to a height of about 1 – 2 feet. This tank size requires an
about 1 kg of Spirulina mother culture. This Spirulina culture along with required minerals should be
released into the tank. The minerals requires for this scenario is 8 grams of sodium bi carbonate plus
5 grams of sodium chloride plus0.2 grams of urea plus 0.5 grams of potassium sulphate plus 0.16
grams of magnesium sulphate plus 0.052 ml of phosphoric acid plus 0.05 ml of ferrous sulphate.
These measurements should be considered for 1 litre of water. After releasing the mother culture and
minerals into the tank, the water should be agitated every day for 1 week using a long stick for 25 to
30 minutes.
o Drying racks needed in case of more production to dry the produce and preserve it.

13. •Light: Growing area (tank/pool/basins) should have proper sunlight as Spirulina is like a plant
and need light to survive.
•Temperature: Requires temperature maintenance in tanks for maximum yield.
•The agitation: Frequent agitation should be carried out to ensure all the Spirulina filaments have
access to Co2 and light.
•The Cultural Medium: Ensure to have right culture medium and perfect water for better growth
and high yield.

14. o After 2 to 3 weeks Spirulina will be ready to pick. Use any small and clean
plastic buckets or a mesh to collect the algae and pour the algae into a
mounted filter. This can squeeze/drain the excess water from harvested
algae. Subsequently, the drained Spirulina should be wrapped in a clean
muslin cloth.
o Press the drained material under a weight of 40 to 50 kg to further drain the
moisture from material. Now you can put in like noodle manufacturing
machines and squeeze in the form of noodles on a dry, clean cloth under the
sun. Allow this to dry further for 2 to3 hours after which it should be ground in
a machine (like a flour machine).
o The ground Spirulina powder ls be sent to the lab for testing. Once
everything is fine, then it should be packed in small airtight plastic covers,
and is ready for market and consumption.

16. Global plant based protein market is expected to reach USD 10,892.3 million by 2022
supported by a CAGR of 6.7% during the forecast period of 2017 to 2022.
Asia Pacific is an emerging market for the plant protein market globally. The
emerging economy of developing nations, increase in population has increased the
protein demand in the region. Plant proteins are the ingredients derived from plant
sources such as soy, wheat, pea, hemp which are rich in amino acid profiles owing to
provide great nutritional benefits on health. Plant protein by the soy source has
contributed the largest market in the region.

17. First, they met with Dr. Siva Subramanian, a
highly respected international algae expert
who handled several industrial projects and
published more than 100 research articles in
national and international journals. He is one
of the core committee of experts on second
generation bio-fuels, Ministry of New and
Renewable Energy (MNRE), Government of
India and member of monitoring committee
member of Council of Scientific and Industrial
research (CSIR) and Department of
Biotechnology (DBT), New Delhi, India for
projects on biofuels from marine microalgae.
He is also a member of PAMC (Project
approval and monitoring committee) of
National Programme on CO2 Sequestration
(NPCS) Research of the Department of
Science and Technology (DST).

22. Estimated costs of starting a 500 sq. m pilot spirulina farm
Serial No. Contents Amounts
1 Total price of land ₹ 160,000
2 Building costs ₹ 240, 000
3. Landscaping ₹ 80, 000
4. Security Lightening ₹ 40, 000
5. Independent production units ₹ 1, 080, 000
6. Shared Structures ₹ 312,000
Total Setup cost: ₹ 1, 912, 000

23. Serial No. Contents Amount
1. Culture cost ₹ 8, 000
2. Medium Preparation fertiliser ₹ 16, 000
3. Farm Manager ₹ 25, 000
4. Lab operator ₹ 20, 000
6. Gardner ₹ 16, 000
7. Guard ₹ 16, 000
8. Total labour cost per month ₹ 18, 000
Total operation Cost per month ₹ 119, 000

24. Serial
Number
Contents Amount
A. Total Expected output (5 kg per
day)
B. Wholesale price per kg (₹ 4, 000)
A + B ₹ 20, 000
C. Direct Cost per day ₹ 3, 967
Gross profit per day ₹ 16, 033
Gross profit per month (30 Days) = 16, 033 * 30 = ₹ 480, 990
Total Expected net profit per month = ₹ 480, 000 - ₹ 119, 000
= ₹ 361, 000
Estimate for Project :
Set up cost + First month operational
cost
= 1, 912, 000 + 119, 000 = 2, 031, 000

28. 1. healthline.com ; Animal vs Plant Protein - What's the Difference?
2. ifpri.org ; Global Hunger Index 2017
3. agrifarming.in ; Growing Spirulina At Home Information
4. paramountspirulina.com ; Health benefit of Spirulina
5. thehindu.com ; Spirulina cultivation: low investment, high income
6. akvopedia.com ; Spirulina farming
7. fao.org ; A review on culture, production and use of Spirulina as food
8. row-organic-spirulina.com ; Exploring Business Opportunities In India With
Spirulina Network
9. bioforsk.no ; Antenna foundation
10.youtube.com ; Visit a local culture of spirulina in Maduari
11. youtube.com ; production and distribution of spirulina (India)