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VAN YÜZÜNCÜ YIL ÜNIVERSITESI
ZIRAAT FAKÜLTESI
TARLA BITKILERI BÖLÜMÜ
The Complete Randomized Block Design
- CRBD -
Sana Jamal SalihMay, 2019
OUTLINES
 DefinitionDefinition
 Details on tDetails on the designhe design
 BlockingBlocking
 Plot Size and ShapePlot Size and Shape
 Randomization StepsRandomization Steps
 Randomization Assignment Of TreatmentsRandomization Assignment Of Treatments
 Advantages and DisadvantagesAdvantages and Disadvantages
 Comparing RCBD with other designsComparing RCBD with other designs
 Statistical AnalysisStatistical Analysis
 ExampleExample
DefinitionDefinition
CRBD is an experimental design for comparingCRBD is an experimental design for comparing tt
treatments andtreatments and bb blocksblocks.. The blocks consist of tThe blocks consist of t
homogeneous experimental units. Treatments arehomogeneous experimental units. Treatments are
randomly assigned to the experimental units within arandomly assigned to the experimental units within a
block, with each treatment appearing exactly once inblock, with each treatment appearing exactly once in
every block.every block.
The Complete Randomized Block Design
- CRBD -
 One of the most widely used experimental designs.
 The design is especially suited for field experiments where
the experimental area has a predictable productivity
gradient.
 The primary distinguishing feature of the design is the
presence of blocks of equal size, each of which contains all
the treatments.
The Complete Randomized Block Design
- CRBD -
 Treatments are assigned randomly within blocks.
 Each treatment replicated only once per block.
 The number of blocks is the number of replications.
 Used to control variation in an experiment by accounting
for spatial effects.
Blocking
 Blocking is used to overcome variability in
the experimental material, e.g:
 Field slopes from East to West
 A fertility gradient across the field
Which orientation of blocks is correct?Which orientation of blocks is correct?
Option IOption I
Direction of variation
(soil fertility)
1 C A B
2 A B C
3 A C B
4 B A C
Option IIOption II
1 2 3 4
A C B C
B A C B
C B A A
Direction of variation
(soil fertility)
Plot Size and ShapePlot Size and Shape
Plot Size
 Practical considerations
 availability of land
 machinery to be used - drill width, spray boom, etc..
 amount of material needed
 cost - larger the plots the greater the cost
 edge effects
 Nature and size of variability
 precision
Plot Shape
 Long and thin or square?
 Long, narrow plots for areas with different fertility.
gradient - length of the plots parallel to the fertility
gradient of the field.
 Where fertility pattern unknown, patchy.
 If edge effects are large, then plots should be
square.
The randomization process for this design is applied
separately and independently to each of the blocks.
Randomization Steps
STEP 1
The experimental area must be divide into equal size blocks.
STEP 2
Subdivide the first block into equal size experimental plots. Number
the plots consecutively from 1 to t, and assign treatments at
random to the plots.
STEP 3
Repeat step 2 completely for each of the remaining blocks.
Randomization Assignment Of
Treatments Per Blocks
Randomization
Advantages
and
Disadvantages
Advantages
1) Complete flexibility; can have any number of treatments and
blocks.
2) Provides more accurate results than the completely
randomized design due to grouping.
3) Relatively easy statistical analysis even with missing data.
Disadvantages
1) Not suitable for large numbers of treatments because blocks
become too large.
2)Not suitable when complete block contains considerable
variability.
3) Interactions between block and treatment effects increase
error.
Comparing RCBD with other designs
Statistical Analysis
Mathematical Model
error random variables
block effects
treatment effects
overall mean
Yij=µ+Τj+βi+εij
ANOVA Table
Source of
Variance
Degrees of
Freedom
Sum of Squares
Mean
Square
F0
Treatments t-1 SSt=∑Yi.2
/b - CF SSt/df MSt/Mse
Blocks b-1 SSb=∑Y.j2
/t - CF SSb/df MSb/Mse
Error (t-1)(b-1) SSe = SST- SSr - SSt SSe/df
Total tb-1 SST=∑Yij2
- CF
Correction factor C.F = Y.. 2 / t b
Multiple
Comparisons
There are many comparison methods
1. Least Significant Difference LSD
2. Tukey’S Test
3. Dunnett's Method
4. Revised Least Significant Difference
5. Duncan's Multiple Range Test
6. Scheffe's F test
7. Welch's (1938) test
8. Mann Whitney-Wilcoxon U test
9. Fligner - Policellotes test
10. Student-Newman-Keuls(SNK) test
and many more ……
Least Significant Difference
1) Uses a t value from tables with the df of the standard error.
2) Most used, and powerful tests available, easy to calculate. (+)
3) Only for comparing mean of two treatments (individual t tests). (-)
4) Only when the result is significant. (-)
Tukey’S Test
1) The test compares every mean with every other mean.
2) To find differences between groups even when the overall ANOVA is not significant.
3) Can be used to determine which means amongst a set of means differ from the rest.
Dunnett's Method
1) Compare a control with each of several other treatments.
2) Can be used even if the result is non-significant.
Example
Effect of Nitrogen fertilizer levels on Sugar
beet yield
BLOCK 1 BLOCK 2 BLOCK 3 BLOCK 4 BLOCK 5
C
(40.9)
A
(33.4)
B
(37.4)
D
(40.1)
C
(39.8)
F
(40.6)
D
(41.7)
C
(39.5)
C
(38.6)
D
(40.0)
E
(39.7)
B
(37.5)
D
(39.4)
E
(38.7)
A
(33.9)
B
(38.8)
F
(41.0)
E
(39.2)
A
(32.2)
B
(38.4)
A
(31.3)
E
(40.6)
F
(41.5)
F
(41.1)
E
(41.9)
D
(40.9)
C
(39.2)
A
(29.2)
B
(35.8)
F
(39.8)
- Treatments
6 Nitrogen fertilizer levels : 0, 50, 100, 150, 200, 250 kg/acer
- Blocks
5 equal size blocks
Layout
- Yield data: tons/acre
- 1 acer = 4.047 decare
Analysis
FIRST STEP
Arrange the treatments
Analysis
Treatments BLOCK 1 BLOCK 2 BLOCK 3 BLOCK 4 BLOCK 5 Total treatment
A
(0 kg)
31.3 33.4 29.2 32.2 33.9 160.0
B
(50 kg)
38.8 37.5 37.4 35.8 38.4 187.9
C
(100 kg)
40.9 39.2 39.5 38.6 39.8 198.0
D
(150 kg)
40.9 41.7 39.4 40.1 40.0 202.1
E
(200 kg)
39.7 40.6 39.2 38.7 41.9 200.1
F
(250 kg)
40.6 41 41.5 41.1 39.8 204.0
Total Blocks 232.2 233.4 226.2 226.5 233.8 1152.1
SECOND STEP
Calculate total
Analysis
1) Correction factor C.F = Y.. 2
/ t b
= (1152.1)2
/(5 x 6) = 44244.48
2) Total sum of squares (SST) = ∑Yij2
– CF
= (31.32
+ 38.82
+ …..+ 39.82
) - 44244.48 = 311.13
3) Block sum of squares (SSb) = ∑Y.j2
/t - CF
= (232.22
+ …… +233.82
)/6- 44244.48 = 9.44
4) Treatment sum of squares (SSt) = ∑Yi.2
/b - CF
= (1602
+ …… +2042
)/5- 44244.48 = 277.69
5) Error sum of squares (SSe) = SST - SSb - SSt = 24
THIRD STEP
Calculate correction factor and sum of squares
Analysis
1) MSb = SSb/(b-1)
= 9.44/4 = 2.36
2) MSt = SSt/(t-1)
= 277.69/5 = 55.54
3) MSe = SSe/(t-1) (b-1)
= 24/(5) (4) = 1.20
FOURTH STEP
Calculate mean squares
FIFTH STEP
Calculate Teble F
1) Ft = MSt/Mse
= 46.28
2) FB = MSb/Mse
= 1.97
ANOVA Table
Source of
Variance
Degrees of
Freedom
Sum of
Squares
Mean Square F0
Treatments 5 277.69 55.54 46.28 **
Blocks 4 9.44 2.36 1.97 n.s
Error 20 24 1.20
Total 29 311.13
Ft: 2.71 (0.05) , 4.10 (0.01)
Fb: 2.87 (0.05) , 4.43 (0.01)
RIGHT choice or NOTRIGHT choice or NOT
Coefficient of variation CV
 It indicates the degree of precision with which the treatments are
compared, and it is a good index of the reliability of the experiment.
 It expresses the experimental error as percentage of the mean
 The lower the cv value, the higher is the reliability of the experiment.
Relative Efficiency
It may be defined in terms of the cost of experimentation, time to collect data,
precision of the data obtained, etc. A commonly used index for comparing the
efficiency of two different designs.
%
%
Thank you for listening
References
1)Montgomery, D.C., 2017. Design and analysis of experiments. John wiley & sons.
2)Ariel, B. and Farrington, D.P., 2014. Randomized block designs. Encyclopedia of
criminology and criminal justice, pp.4273-4283.
3)Clewer, A.G. and Scarisbrick, D.H., 2013. Practical statistics and experimental design for
plant and crop science. John Wiley & Sons.
4)Toutenburg, H., 2009. Statistical analysis of designed experiments. Springer Science &
Business Media.
5)Cox, D.R. and Reid, N., 2000. The Theory of the Design of Experiments. CHAPMAN &
HALL/CRC.
6)Gomez, K.A. and Gomez, A.A., 1984. Statistical procedures for agricultural research.
John Wiley & Sons.
7)
8)
9)http://influentialpoints.com/Training/coefficient_of_variation-principles-properties-assump
htm

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Complete randomized block design - Sana Jamal Salih

  • 1. VAN YÜZÜNCÜ YIL ÜNIVERSITESI ZIRAAT FAKÜLTESI TARLA BITKILERI BÖLÜMÜ The Complete Randomized Block Design - CRBD - Sana Jamal SalihMay, 2019
  • 2. OUTLINES  DefinitionDefinition  Details on tDetails on the designhe design  BlockingBlocking  Plot Size and ShapePlot Size and Shape  Randomization StepsRandomization Steps  Randomization Assignment Of TreatmentsRandomization Assignment Of Treatments  Advantages and DisadvantagesAdvantages and Disadvantages  Comparing RCBD with other designsComparing RCBD with other designs  Statistical AnalysisStatistical Analysis  ExampleExample
  • 3. DefinitionDefinition CRBD is an experimental design for comparingCRBD is an experimental design for comparing tt treatments andtreatments and bb blocksblocks.. The blocks consist of tThe blocks consist of t homogeneous experimental units. Treatments arehomogeneous experimental units. Treatments are randomly assigned to the experimental units within arandomly assigned to the experimental units within a block, with each treatment appearing exactly once inblock, with each treatment appearing exactly once in every block.every block.
  • 4. The Complete Randomized Block Design - CRBD -  One of the most widely used experimental designs.  The design is especially suited for field experiments where the experimental area has a predictable productivity gradient.  The primary distinguishing feature of the design is the presence of blocks of equal size, each of which contains all the treatments.
  • 5. The Complete Randomized Block Design - CRBD -  Treatments are assigned randomly within blocks.  Each treatment replicated only once per block.  The number of blocks is the number of replications.  Used to control variation in an experiment by accounting for spatial effects.
  • 6. Blocking  Blocking is used to overcome variability in the experimental material, e.g:  Field slopes from East to West  A fertility gradient across the field
  • 7. Which orientation of blocks is correct?Which orientation of blocks is correct?
  • 8. Option IOption I Direction of variation (soil fertility) 1 C A B 2 A B C 3 A C B 4 B A C
  • 9. Option IIOption II 1 2 3 4 A C B C B A C B C B A A Direction of variation (soil fertility)
  • 10. Plot Size and ShapePlot Size and Shape
  • 11. Plot Size  Practical considerations  availability of land  machinery to be used - drill width, spray boom, etc..  amount of material needed  cost - larger the plots the greater the cost  edge effects  Nature and size of variability  precision
  • 12. Plot Shape  Long and thin or square?  Long, narrow plots for areas with different fertility. gradient - length of the plots parallel to the fertility gradient of the field.  Where fertility pattern unknown, patchy.  If edge effects are large, then plots should be square.
  • 13. The randomization process for this design is applied separately and independently to each of the blocks. Randomization Steps STEP 1 The experimental area must be divide into equal size blocks. STEP 2 Subdivide the first block into equal size experimental plots. Number the plots consecutively from 1 to t, and assign treatments at random to the plots. STEP 3 Repeat step 2 completely for each of the remaining blocks.
  • 14. Randomization Assignment Of Treatments Per Blocks Randomization
  • 16. Advantages 1) Complete flexibility; can have any number of treatments and blocks. 2) Provides more accurate results than the completely randomized design due to grouping. 3) Relatively easy statistical analysis even with missing data.
  • 17. Disadvantages 1) Not suitable for large numbers of treatments because blocks become too large. 2)Not suitable when complete block contains considerable variability. 3) Interactions between block and treatment effects increase error.
  • 18. Comparing RCBD with other designs
  • 20. Mathematical Model error random variables block effects treatment effects overall mean Yij=µ+Τj+βi+εij
  • 21. ANOVA Table Source of Variance Degrees of Freedom Sum of Squares Mean Square F0 Treatments t-1 SSt=∑Yi.2 /b - CF SSt/df MSt/Mse Blocks b-1 SSb=∑Y.j2 /t - CF SSb/df MSb/Mse Error (t-1)(b-1) SSe = SST- SSr - SSt SSe/df Total tb-1 SST=∑Yij2 - CF Correction factor C.F = Y.. 2 / t b
  • 23. There are many comparison methods 1. Least Significant Difference LSD 2. Tukey’S Test 3. Dunnett's Method 4. Revised Least Significant Difference 5. Duncan's Multiple Range Test 6. Scheffe's F test 7. Welch's (1938) test 8. Mann Whitney-Wilcoxon U test 9. Fligner - Policellotes test 10. Student-Newman-Keuls(SNK) test and many more ……
  • 24. Least Significant Difference 1) Uses a t value from tables with the df of the standard error. 2) Most used, and powerful tests available, easy to calculate. (+) 3) Only for comparing mean of two treatments (individual t tests). (-) 4) Only when the result is significant. (-)
  • 25. Tukey’S Test 1) The test compares every mean with every other mean. 2) To find differences between groups even when the overall ANOVA is not significant. 3) Can be used to determine which means amongst a set of means differ from the rest.
  • 26. Dunnett's Method 1) Compare a control with each of several other treatments. 2) Can be used even if the result is non-significant.
  • 27. Example Effect of Nitrogen fertilizer levels on Sugar beet yield
  • 28. BLOCK 1 BLOCK 2 BLOCK 3 BLOCK 4 BLOCK 5 C (40.9) A (33.4) B (37.4) D (40.1) C (39.8) F (40.6) D (41.7) C (39.5) C (38.6) D (40.0) E (39.7) B (37.5) D (39.4) E (38.7) A (33.9) B (38.8) F (41.0) E (39.2) A (32.2) B (38.4) A (31.3) E (40.6) F (41.5) F (41.1) E (41.9) D (40.9) C (39.2) A (29.2) B (35.8) F (39.8) - Treatments 6 Nitrogen fertilizer levels : 0, 50, 100, 150, 200, 250 kg/acer - Blocks 5 equal size blocks Layout - Yield data: tons/acre - 1 acer = 4.047 decare
  • 30. Analysis Treatments BLOCK 1 BLOCK 2 BLOCK 3 BLOCK 4 BLOCK 5 Total treatment A (0 kg) 31.3 33.4 29.2 32.2 33.9 160.0 B (50 kg) 38.8 37.5 37.4 35.8 38.4 187.9 C (100 kg) 40.9 39.2 39.5 38.6 39.8 198.0 D (150 kg) 40.9 41.7 39.4 40.1 40.0 202.1 E (200 kg) 39.7 40.6 39.2 38.7 41.9 200.1 F (250 kg) 40.6 41 41.5 41.1 39.8 204.0 Total Blocks 232.2 233.4 226.2 226.5 233.8 1152.1 SECOND STEP Calculate total
  • 31. Analysis 1) Correction factor C.F = Y.. 2 / t b = (1152.1)2 /(5 x 6) = 44244.48 2) Total sum of squares (SST) = ∑Yij2 – CF = (31.32 + 38.82 + …..+ 39.82 ) - 44244.48 = 311.13 3) Block sum of squares (SSb) = ∑Y.j2 /t - CF = (232.22 + …… +233.82 )/6- 44244.48 = 9.44 4) Treatment sum of squares (SSt) = ∑Yi.2 /b - CF = (1602 + …… +2042 )/5- 44244.48 = 277.69 5) Error sum of squares (SSe) = SST - SSb - SSt = 24 THIRD STEP Calculate correction factor and sum of squares
  • 32. Analysis 1) MSb = SSb/(b-1) = 9.44/4 = 2.36 2) MSt = SSt/(t-1) = 277.69/5 = 55.54 3) MSe = SSe/(t-1) (b-1) = 24/(5) (4) = 1.20 FOURTH STEP Calculate mean squares FIFTH STEP Calculate Teble F 1) Ft = MSt/Mse = 46.28 2) FB = MSb/Mse = 1.97
  • 33. ANOVA Table Source of Variance Degrees of Freedom Sum of Squares Mean Square F0 Treatments 5 277.69 55.54 46.28 ** Blocks 4 9.44 2.36 1.97 n.s Error 20 24 1.20 Total 29 311.13 Ft: 2.71 (0.05) , 4.10 (0.01) Fb: 2.87 (0.05) , 4.43 (0.01)
  • 34. RIGHT choice or NOTRIGHT choice or NOT
  • 35. Coefficient of variation CV  It indicates the degree of precision with which the treatments are compared, and it is a good index of the reliability of the experiment.  It expresses the experimental error as percentage of the mean  The lower the cv value, the higher is the reliability of the experiment.
  • 36. Relative Efficiency It may be defined in terms of the cost of experimentation, time to collect data, precision of the data obtained, etc. A commonly used index for comparing the efficiency of two different designs. % %
  • 37. Thank you for listening
  • 38. References 1)Montgomery, D.C., 2017. Design and analysis of experiments. John wiley & sons. 2)Ariel, B. and Farrington, D.P., 2014. Randomized block designs. Encyclopedia of criminology and criminal justice, pp.4273-4283. 3)Clewer, A.G. and Scarisbrick, D.H., 2013. Practical statistics and experimental design for plant and crop science. John Wiley & Sons. 4)Toutenburg, H., 2009. Statistical analysis of designed experiments. Springer Science & Business Media. 5)Cox, D.R. and Reid, N., 2000. The Theory of the Design of Experiments. CHAPMAN & HALL/CRC. 6)Gomez, K.A. and Gomez, A.A., 1984. Statistical procedures for agricultural research. John Wiley & Sons. 7) 8) 9)http://influentialpoints.com/Training/coefficient_of_variation-principles-properties-assump htm