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SIKSHA‘O’ANUSANDHAN
INSTITUTE OFAGRICULTURAL SCIENCES
TOPIC:CROPIMPROVEMENTINSUNFLOWER
Presented By: Ritabrata
Sarkar
Regd. No. 1741901024
B. Sc Ag, 3rd year
Submitted to- Chinmayee Behera
Divya Bharti Sahu
Asst. Prof.
Dept. Of Plant Breeding and Genetics
INTRODUCTION
• Sunflower is a most important oil crop in world.
• Sunflower oil is used as a substitute for mineral oil in various applications such as
fuel or an oil for hydraulic system.
• Oil content ranges from 29-30 per cent and is of high quality having non-cholesteral
properties.
• The stakes of the head are used to make compost.
• Oil extracted from sunflower is edible as it is rich in un-saturated fatty acids like oleic
(19%) and linoleic acids (68%).
SUNFLOWER
◦ Scientific name: Helianthus annuus; Helianthus tuberosus
◦ Family: Asteraceae
◦ Order : Asterales
◦ Chromosome number: H. annuus (2n=2x=34)
H. tuberosus (2n=6x=102)
CENTRE OF ORIGIN
Central America. This hypothesis is based on archaeological evidence.
DISTRIBUTION
◦ Soviet Union, Romania, Canada, USA.
◦ In India this crop is introduced in 1969 from Soviet Union.
◦ In India it is cultivated in Tamil Nadu, Karnataka, Maharashtra and Andhra Pradesh,
Punjab and Haryana.
 Progenitor: Helianthus petiolaris
Helianthus gigants
 Wild species: Helianthus hirsutus
Helianthus rigidus
 The genus Helianthus comprises of 67 species.
 Two species H. annuus and H. tuberosus are cultivated as food plants.
 Genus has basic chromosome number of 17 and diploid, tetraploid and hexaploid
species are found.
Fatty Acid Composition
Oil
Content
(%)
Lauric
Acid
(12:0)
Myristic
Acid
(14:0)
Palmitic
Acid
(16:0)
Stearic
Acid
(18:0)
Oleic
Acid
(18:1)
Linoleic
Acid
(18:2)
Linolenic
Acid
(18:3)
Eicosenoic
Acid
(20:1)
Erusi
c
Acid
(22:1)
Total
unsaturated
fatty acids
(%)
29 - - 5.6 6.5 19.1 67 < 0.5 - - 86.1
(g/100g fatty acid)
(Based on: Mitra and Bhatia, 1979 and Khanna and Singh, 1991)
Cultivars of Sunflower
1) Giant types: 6 - 14 feet tall. Late maturing, Large heads 12 - 30” in diameter, seeds
large, white or grey or with black stripes. Oil content is very low. E.g. Mammoth
Russian.
2) Semi dwarf varieties: Medium tall - 4 ½ to 6 feet, Early maturing. Heads 7 - 9” in
diameter. Seeds smaller, black, grey or striped. High oil content 35%. E.g. Jupiter,
Pole star.
3) Dwarf types: 2 to 4½ feet tall. Early maturing. Head size 5½ - 6½ “ diameter. Small
seeds, high oil content 37%. E.g. Sunrise, Morden, Co1, Co2
BOTANY
It is distinguished from all other cultivated plants by its single stem and large
inflorescence.
The inflorescence is head or capitulum.
It composed of many individual florets i.e. Ray floret and disc floret.
Ray floret: Flowers of the outer whorl; infertile florets.
Disc floret: Arranged in inner side of ray florets; fertile florets.
It is having a bisexual flower.
FLORAL MORPHOLOGY
CYTOLOGY
• Its includes diploid, tetraploid ,and hexaploid, species.
• The 17 chromosomes are divided into four group based on the position of
centromere.
• The length of the 17 chromosome varies from 3.05 to 6.20 microns.
FLORAL BIOLOGY
 It is protandrous in nature.
 Anthesis begins with ray florets and proceeds towards the disc florets.
 2-4 whorls open daily and total anthesis take 5-8 days.
 Anthesis is delay when the weather is cloudy, humid and cool.
 Flowering starts in the early morning 6-10 am.
 Pollen viability up to 12 hrs.
 Stigma receptivity up to 2-3 days.
BREEDING OBJECTIVES
1) To develop short duration varieties suitable for dry
land and irrigated conditions:
• Dryland successful in black soils only.
• In red soil under rainfed it is not successful.
2) High oil content:
 Ranges 38 to 48%.
 Complex character.
 Yield and oil content are negatively correlated.
3) Protein content and hullability:
◦ Another seed quality importance given to seed protein content.
◦ There is generally a negative correlation between oil and protein contents.
◦ protein content can be increased by increase the hullability of seed.
4)Breeding for fatty acid:
A further important quality character for sunflowers is the proportion of the different fatty
acids (mainly unsaturated fatty acid) in oil.
5)Breeding for self fertile lines:
• Protoandry and self incompatibility mechanism operates in sunflower.
• Hence hand pollination is necessary.
• To avoid this self fertile lines can be evolved.
6) Breeding for disease resistance:
 Maharashtra hybrid susceptible to powdery mildew. Hence ban is there.
 Powdery mildew, rust, charcoal rot, Alternaria blight are the major diseases.
 Wild species like H. hirsuta are moderately resistance to Alternaria.
7) Pest and insects resistance variety:
• The crop is attack by different insect and pests, which causes severe losses in
production.
• So it become necessary to developed insect pest resistance variety to increase yield.
• Different pests attack to sunflower crop a are Heliothis , Grass hopper, Jassids.
8) Height, earliness, changes in architecture or
vegetation cycle:
 Plant height at flowering, flowering date and per cent humidity of seed at
harvest are characters regularly measured in sunflower breeding programmes.
Mostly to avoid very tall or very late material and to have a good knowledge of an
inbred line or a hybrid.
 Short sunflowers could be sown at higher density and thus increase yield, with a
crop of many small heads, covering the ground more quickly in spring and maturing
more rapidly, permitting an earlier harvest.
9) Minor constituents:
Minor constituents like tocopherols and phytosterols should be increased to provide
a significant increased value.
BREEDING METHODS
 Introduction :
Established plants of an area are taken to the newer area for the use of mankind.
Eg: Morden from Canada.
 Mass selection :
In this method phenotypically desirable plants are selected and harvested from a
population and their seeds are mixed to constitute a new cultivar or a superior
source population.
E.g. Ec 68414 from Russia.
 Hybridization and selection :
Intervarietal: Hybrids obtained by crossing parents belonging to same species;
may be 2 varities or races of the same species.
◦ Currently there is increase emphasis in development of sunflower hybrid.
◦ For production of hybrid seed cytoplasmic genetic male sterile lines are used.
 Mutation :
Co3 (Mutant from Co2 through gamma rays)
 Heterosis breeding :
Development of inbred lines and crossing them to harness heterosis was first done as
early as 1920 in Russia.
During 1970 cytoplasmic geneic male sterility was identified in wild types and obsolete
cultivars. Now this system is being extensively used for production of hybrids.
 Eg: BSH-1, APSH -11
Oil and hull analysis. 1000-1200 heads are selected from the original
population. From the separately harvested seeds half seeds are
reserved and half seeds are sown.
Growing of 1000-1200 plants.
Evaluation for agronomic traits and resistance to diseases. Selection
of 150-200 heads of superior quality.
Reserve seeds from the original plants are used to plant for second
year, based on progeny performance. Second year evaluation of
characters and selection of 20-50 heads for cross pollination.
Growing of 20-50 selected plants. Removal of undesirable plants and
selection of best plants within 20-50 progenies.
Mixing of seeds of selected plants to build and test preliminary strain.
 Head to row and remnant seed method :
Original Population
Developed by Pustovoit in Russia. By this method oil content is increased.
Yield Trial
ARTIFICAL SELFING & CROSSING
TECHNIQUES
Selfing
Selfing is done by bagging of the entire head. The bagging material could be cotton
cloth or paper bag or cheese cloth bag.
Crossing Techniques
1.Hand emasculation:
• Emasculation is done by removing the anther tubes with forceps on the morning that the
flowers open. Unemasculated flowers are removed.
2.Chemical induction of male sterility:
• This is achieved by spraying of 0.05% solution of gibberellic acid per plant during bud
initiation.
• Application of gibberellic acid after bud initiation consecutively for 3 days in the morning
gives better results.
 Pollination is carried out by collecting the pollen from the male parent which are
already bagged prior to flowering.
 Pollen can be collected from the flowering head. Pollination is usually done in the
same morning after emasculation.
Hybrid Seed Production
Sowing Season: Kharif crop should be sown during June-July and rabi crop during
December-January.
Isolation: 600 m
Spacing: 60cm × 30cm
Seed rate: About 7.5 kg of CMS 234 (female) and 2.5 kg of RHA 274 (male)
HYBRIDS AND THEIR
PARENTAGES
• BSH-1 = (CMS-234A X RHA-274)
• KBSH-41= (CMS-234A X RHA-95-C-1)
• KBSH-42= (CMS-851A X RHA-95-C-1)
• KBSH-44= (CMS-17A X RHA-95-C-1)
• Now, KBSH-53, 55, 58, 62 are released for Karnataka state.
VARITIES OF SUNFLOWER
VARITEY STATES DURATION (Days) SEED YIELD (Kg/ha)
BSH 1 All 85-90 1000-1500
KBSH 1 All 90-95 1200-1500
LSH 1 Maharashtra 85-90 900-1200
PSFH 67 Punjab 90-95 1000-1500
PKVSH 27 Punjab 75-80 900-1100
MODERN All 70-80 600-800
EC 68414 Karnataka 100-110 800-1000
SURYA Maharashtra 90-95 800-1000
CO 1 Tamil Nadu 65-70 500-700
CO 2 Tamil Nadu 85-90 800-1000
AICRP ON SUNFLOWER AND
VARITIES
AICRP on sunflower was established in the year 1972,with head quarters at UAS,
Bangalore.
Directorate of oil seed Research (DOR), Hyderabad.
 1974- No. of hybrids were developed by CMS lines. BSH1 and BSH2 were found
promising hybrids among them.
1975- EC-68414, 68415 for cultivation.
1978 -Morden –Early duration variety.
FUTURE PROSPECTS
 Sunflower is one of the fastest developing oil seed crop. It was introduced in the year
1969.
 Today more than 1 million ha area is under sunflower cultivation.
 The productivity of sunflower is quite low in India (4-6 q/ha) as compared to other
countries.
REFERENCE
◦ Ram H. H., 2016. Crop Breeding and Biotechnology, Sunflower, pp 394-408, Kalyani
Publishers.
◦ Singh, B. D., 2019. Plant Breeding Principles and Methods, pp 563-583, Kalyani Publishers
◦ Sindagi, S. S., Virupaskshappa, K.1986. Sunflower. Indian Council of Agricultural Research,
Krishi Bhavan
Crop improvement techniques in sunflower breeding

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Crop improvement techniques in sunflower breeding

  • 1. SIKSHA‘O’ANUSANDHAN INSTITUTE OFAGRICULTURAL SCIENCES TOPIC:CROPIMPROVEMENTINSUNFLOWER Presented By: Ritabrata Sarkar Regd. No. 1741901024 B. Sc Ag, 3rd year Submitted to- Chinmayee Behera Divya Bharti Sahu Asst. Prof. Dept. Of Plant Breeding and Genetics
  • 2. INTRODUCTION • Sunflower is a most important oil crop in world. • Sunflower oil is used as a substitute for mineral oil in various applications such as fuel or an oil for hydraulic system. • Oil content ranges from 29-30 per cent and is of high quality having non-cholesteral properties. • The stakes of the head are used to make compost. • Oil extracted from sunflower is edible as it is rich in un-saturated fatty acids like oleic (19%) and linoleic acids (68%).
  • 3. SUNFLOWER ◦ Scientific name: Helianthus annuus; Helianthus tuberosus ◦ Family: Asteraceae ◦ Order : Asterales ◦ Chromosome number: H. annuus (2n=2x=34) H. tuberosus (2n=6x=102)
  • 4. CENTRE OF ORIGIN Central America. This hypothesis is based on archaeological evidence. DISTRIBUTION ◦ Soviet Union, Romania, Canada, USA. ◦ In India this crop is introduced in 1969 from Soviet Union. ◦ In India it is cultivated in Tamil Nadu, Karnataka, Maharashtra and Andhra Pradesh, Punjab and Haryana.
  • 5.  Progenitor: Helianthus petiolaris Helianthus gigants  Wild species: Helianthus hirsutus Helianthus rigidus  The genus Helianthus comprises of 67 species.  Two species H. annuus and H. tuberosus are cultivated as food plants.  Genus has basic chromosome number of 17 and diploid, tetraploid and hexaploid species are found.
  • 7. Cultivars of Sunflower 1) Giant types: 6 - 14 feet tall. Late maturing, Large heads 12 - 30” in diameter, seeds large, white or grey or with black stripes. Oil content is very low. E.g. Mammoth Russian. 2) Semi dwarf varieties: Medium tall - 4 ½ to 6 feet, Early maturing. Heads 7 - 9” in diameter. Seeds smaller, black, grey or striped. High oil content 35%. E.g. Jupiter, Pole star. 3) Dwarf types: 2 to 4½ feet tall. Early maturing. Head size 5½ - 6½ “ diameter. Small seeds, high oil content 37%. E.g. Sunrise, Morden, Co1, Co2
  • 8. BOTANY It is distinguished from all other cultivated plants by its single stem and large inflorescence. The inflorescence is head or capitulum. It composed of many individual florets i.e. Ray floret and disc floret. Ray floret: Flowers of the outer whorl; infertile florets. Disc floret: Arranged in inner side of ray florets; fertile florets. It is having a bisexual flower.
  • 10. CYTOLOGY • Its includes diploid, tetraploid ,and hexaploid, species. • The 17 chromosomes are divided into four group based on the position of centromere. • The length of the 17 chromosome varies from 3.05 to 6.20 microns.
  • 11. FLORAL BIOLOGY  It is protandrous in nature.  Anthesis begins with ray florets and proceeds towards the disc florets.  2-4 whorls open daily and total anthesis take 5-8 days.  Anthesis is delay when the weather is cloudy, humid and cool.  Flowering starts in the early morning 6-10 am.  Pollen viability up to 12 hrs.  Stigma receptivity up to 2-3 days.
  • 12. BREEDING OBJECTIVES 1) To develop short duration varieties suitable for dry land and irrigated conditions: • Dryland successful in black soils only. • In red soil under rainfed it is not successful. 2) High oil content:  Ranges 38 to 48%.  Complex character.  Yield and oil content are negatively correlated.
  • 13. 3) Protein content and hullability: ◦ Another seed quality importance given to seed protein content. ◦ There is generally a negative correlation between oil and protein contents. ◦ protein content can be increased by increase the hullability of seed. 4)Breeding for fatty acid: A further important quality character for sunflowers is the proportion of the different fatty acids (mainly unsaturated fatty acid) in oil. 5)Breeding for self fertile lines: • Protoandry and self incompatibility mechanism operates in sunflower. • Hence hand pollination is necessary. • To avoid this self fertile lines can be evolved.
  • 14. 6) Breeding for disease resistance:  Maharashtra hybrid susceptible to powdery mildew. Hence ban is there.  Powdery mildew, rust, charcoal rot, Alternaria blight are the major diseases.  Wild species like H. hirsuta are moderately resistance to Alternaria. 7) Pest and insects resistance variety: • The crop is attack by different insect and pests, which causes severe losses in production. • So it become necessary to developed insect pest resistance variety to increase yield. • Different pests attack to sunflower crop a are Heliothis , Grass hopper, Jassids.
  • 15. 8) Height, earliness, changes in architecture or vegetation cycle:  Plant height at flowering, flowering date and per cent humidity of seed at harvest are characters regularly measured in sunflower breeding programmes. Mostly to avoid very tall or very late material and to have a good knowledge of an inbred line or a hybrid.  Short sunflowers could be sown at higher density and thus increase yield, with a crop of many small heads, covering the ground more quickly in spring and maturing more rapidly, permitting an earlier harvest. 9) Minor constituents: Minor constituents like tocopherols and phytosterols should be increased to provide a significant increased value.
  • 16. BREEDING METHODS  Introduction : Established plants of an area are taken to the newer area for the use of mankind. Eg: Morden from Canada.  Mass selection : In this method phenotypically desirable plants are selected and harvested from a population and their seeds are mixed to constitute a new cultivar or a superior source population. E.g. Ec 68414 from Russia.  Hybridization and selection : Intervarietal: Hybrids obtained by crossing parents belonging to same species; may be 2 varities or races of the same species. ◦ Currently there is increase emphasis in development of sunflower hybrid. ◦ For production of hybrid seed cytoplasmic genetic male sterile lines are used.
  • 17.  Mutation : Co3 (Mutant from Co2 through gamma rays)  Heterosis breeding : Development of inbred lines and crossing them to harness heterosis was first done as early as 1920 in Russia. During 1970 cytoplasmic geneic male sterility was identified in wild types and obsolete cultivars. Now this system is being extensively used for production of hybrids.  Eg: BSH-1, APSH -11
  • 18. Oil and hull analysis. 1000-1200 heads are selected from the original population. From the separately harvested seeds half seeds are reserved and half seeds are sown. Growing of 1000-1200 plants. Evaluation for agronomic traits and resistance to diseases. Selection of 150-200 heads of superior quality. Reserve seeds from the original plants are used to plant for second year, based on progeny performance. Second year evaluation of characters and selection of 20-50 heads for cross pollination. Growing of 20-50 selected plants. Removal of undesirable plants and selection of best plants within 20-50 progenies. Mixing of seeds of selected plants to build and test preliminary strain.  Head to row and remnant seed method : Original Population Developed by Pustovoit in Russia. By this method oil content is increased. Yield Trial
  • 19. ARTIFICAL SELFING & CROSSING TECHNIQUES Selfing Selfing is done by bagging of the entire head. The bagging material could be cotton cloth or paper bag or cheese cloth bag. Crossing Techniques 1.Hand emasculation: • Emasculation is done by removing the anther tubes with forceps on the morning that the flowers open. Unemasculated flowers are removed. 2.Chemical induction of male sterility: • This is achieved by spraying of 0.05% solution of gibberellic acid per plant during bud initiation. • Application of gibberellic acid after bud initiation consecutively for 3 days in the morning gives better results.
  • 20.  Pollination is carried out by collecting the pollen from the male parent which are already bagged prior to flowering.  Pollen can be collected from the flowering head. Pollination is usually done in the same morning after emasculation.
  • 21. Hybrid Seed Production Sowing Season: Kharif crop should be sown during June-July and rabi crop during December-January. Isolation: 600 m Spacing: 60cm × 30cm Seed rate: About 7.5 kg of CMS 234 (female) and 2.5 kg of RHA 274 (male)
  • 22. HYBRIDS AND THEIR PARENTAGES • BSH-1 = (CMS-234A X RHA-274) • KBSH-41= (CMS-234A X RHA-95-C-1) • KBSH-42= (CMS-851A X RHA-95-C-1) • KBSH-44= (CMS-17A X RHA-95-C-1) • Now, KBSH-53, 55, 58, 62 are released for Karnataka state.
  • 23. VARITIES OF SUNFLOWER VARITEY STATES DURATION (Days) SEED YIELD (Kg/ha) BSH 1 All 85-90 1000-1500 KBSH 1 All 90-95 1200-1500 LSH 1 Maharashtra 85-90 900-1200 PSFH 67 Punjab 90-95 1000-1500 PKVSH 27 Punjab 75-80 900-1100 MODERN All 70-80 600-800 EC 68414 Karnataka 100-110 800-1000 SURYA Maharashtra 90-95 800-1000 CO 1 Tamil Nadu 65-70 500-700 CO 2 Tamil Nadu 85-90 800-1000
  • 24. AICRP ON SUNFLOWER AND VARITIES AICRP on sunflower was established in the year 1972,with head quarters at UAS, Bangalore. Directorate of oil seed Research (DOR), Hyderabad.  1974- No. of hybrids were developed by CMS lines. BSH1 and BSH2 were found promising hybrids among them. 1975- EC-68414, 68415 for cultivation. 1978 -Morden –Early duration variety.
  • 25. FUTURE PROSPECTS  Sunflower is one of the fastest developing oil seed crop. It was introduced in the year 1969.  Today more than 1 million ha area is under sunflower cultivation.  The productivity of sunflower is quite low in India (4-6 q/ha) as compared to other countries.
  • 26. REFERENCE ◦ Ram H. H., 2016. Crop Breeding and Biotechnology, Sunflower, pp 394-408, Kalyani Publishers. ◦ Singh, B. D., 2019. Plant Breeding Principles and Methods, pp 563-583, Kalyani Publishers ◦ Sindagi, S. S., Virupaskshappa, K.1986. Sunflower. Indian Council of Agricultural Research, Krishi Bhavan