Introduction Vaccines do? History Types Stages Conclusion

1. VACCINE PRODUCTION
PRESENTED BY-
NILAMBAR KHURA
M.SC IN ZOOLOGY
18PZOL12
GOVT.AUTONOMOUS COLLEGE,ROURKELA

2. CONTENTS
 Introduction
 History
 What Vaccines do?
 Types of Vaccine
 Production of Vaccine
 Significance
 Reference

3. INTRODUCTION
•Vaccine may be defined as the agent which enhance
the active immunity of the body.
•They give us a healthy defense.
•The vaccine used to treat for a particular infection is
called “vaccination”.

4. HISTORY
•The word ‘Vaccine” originates from the Latin
word vaccinus .
•Edward Jenner demonstrated in 1798 could
prevent smallpox in humans.
•Today the term “Vaccine” applies to all
biological preparations, produced from living
organisms, that enhance immunity against
disease.Edward Jenner
1749-1823

5. What vaccines do?
 When inactivated or weakened disease- causing microorganisms enter
the body, they initiate an immune response.
 This response mimics the body’s natural response to infection.
 These antigens triggers the production of Antibodies by the immune
system.
 Antibodies bind to corresponding antigens and induce their
destruction by other immune cells.

6. Source –www.sonofi.co.in
TYPES OF VACCINES

7. Production of vaccine
 Vaccines are produced in large scale as they need to be
administrated to large populations of children and
adults to be effective as a public health tool.
 For the production of vaccines has several stages
through which vaccine produced.

8. Stages of vaccine production
1. Generation /Isolation of Antigen
2. Purification
3. Inactivation
4. Formulation
5. Filling
6. Batch release
7. Packing and shipping

9. Stages of vaccine production
Sourse-www.snofi.com

10. 01.Generation/Isolation of antigen
•Viruses are grown on primary cells such as cells from chicken embryos or using
fertilized eggs (e. g. influenza vaccine)or cell lines that reproduce repeatedly( e.g.
hepatitis A).
•Bacteria are grown in bioreactors which are devices that use a particular growth
medium that optimises the production of the antigens
•Recombinant proteins derived from the pathogen can be generated either in
yeast, bacteria or cell cultures.
•Production starts by growing viruses bacteria in the lab a wide range of program
meter manages like –
Temperatures
PH
Oxygen Rate
Sterility
Homogeneity

11. 02.Purification
 The microorganisms are extracted from the
environment through a process called purification
which are ruminates any traces in culture media.

12. 03.Inactivation
 Then inactivate the virus or Bacteria that ruminates to
ability to cause disease.
 We retain its ability to leisure precautionary immune
response from the body.
 That activated virus or bacteria is called a valence.

13. 04.Formulation
 Once the antigen is developed the vaccine is
formulated by adding adjuvant, stabilizers, and
preservatives.
 The role of the adjuvant is to enhance the immune
response of the antigen.
 The stabilizers increase the storage life, and
preservatives use of allow multi dose vials.

14. 05.Filling
 When its times for the vaccine to be administrated it
would be reconstituted by combining the powder with
diluents.
 The vaccine is liquid or freeze dried form and diluents
is needed and then filled into seeds or syringes .
 Each phase undergoes visual inspection.
 Quality containers are scrutinized by human eye and
sterile in digital surveillance technology.

15. 06.Batch release
 Samples are taken from each batch.
 After vaccines are successfully passed both sides of test
distribution began.
 No exception if a batch is not matched with quality
criteria will be destroyed.

16. 07.Packing and shipping
 For the packing and shipping vaccine must be stored in 20 to 80 C.
 Temperatures tracking device is included in each shipment.

17. Significance of vaccine production
 Makes good economic sense, and meets the need to
care for the weakest members of societies.
 Reducing global child mortality.
 Global life saving immunization against deadly dieses
like meales, Hib and rotavirus.

18. REFERENCES
1. http://www.biotec.or.th/biosafety/download/biotec%20seminar%202.pdf
2. http://www.cfsciences.com/pdf?3.%20vaccine%20production%20ver7.pdf
3. www.hsc.gov.sg
4. www.sonofi.com
5. www.sciencedirect.com
6. www.shareyourarticles.com
7. Life sciences: Fundamental and practice[5th edition]