1. Curriculum Vitae
Nikeh Shariatian
Personal
Information
Gender: Female
Date of Birth:24/02/1980
Marital Status: Married
Telephone: 03 85722806
Mobile: 0430653656
Email: nikeh.shariatian@hudson.org.au
Home Address: 25 Willow Ave Rowville Vic 3178
Work Address: 27-31 Wright St Clayton Vic 3168
Tertiary
Qualification
Sep 2003 - Sep 2006, Azad University , Tehran, Iran
MSc. Majoring in Cellular and Molecular Biology
Sep 1998 - June 2002, Azad University, Tehran, Iran
BSc. Majoring in Microbiology
Work
Experiences
July 2010- Present,
Hudson Institute of Medical Research and Monash Institute of Medical Research
The Ritchie Centre Position: Research Assistant
• July 2010- December 2014 - Monash Institute of Medical Research
The Ritchie Centre - Nold Group
• January 2015 - Present - Hudson Institute of Medical Research
The Ritchie Centre - Perinatal Inflammation Group
Responsibilities:
• Performing “Molecular Techniques” such as Real time PCR, DNA & RNA extraction
techniques in aseptic conditions, Polymerase Chain Reaction and gel
electrophoresis DNA analysis
• Protein extraction techniques, Western hybridization analysis using SDS PAGE,
performing protein assays, ELISA and Proteome Profiler assays
• Tissue and cell culture techniques such as isolating Splenocytes and Dendritic Cells
from Mouse Tissue, culturing cell lines, isolating mononuclear cells from human
blood samples
• Histology techniques such as Embedding, cutting and H&E staining
• Analyzing biological images using software such as Image J and Image Scope
• Handling and working with laboratory animals such as mice
• Keeping proper and detailed records of day to day lab procedures and experiments
• Ordering all the materials for the lab using the Hudson ordering system
• Communicating with suppliers on purchasing chemicals and equipment
• Managing national and international shipments
• Preparing Animal and Human Ethics applications, grants and drafts for papers.
• Providing inductions to staff and students in compliance with Hudson Institute
rules and regulations
• Being in charge of the keeping and running of the Protein laboratory at The Ritchie
Centre
• Being appointed as the Fire warden and Safety Officer of Monash University located
at MIMR

2. • Being appointed as a member of the Social Committee of the Hudson Institute of
Medical Research
• Keeping to standard operation procedures (SOPs)
February 2005 - June 2009, Alzahra University, Tehran, Iran
Position: Research Assistant (Molecular Biology)
Responsibilities:
• DNA & RNA extraction techniques in aseptic conditions including phenol
choloroform
• Design of oligonucleotides using NetPrimer and BlastN
• Polymerase Chain Reaction and gel electrophoresis DNA analysis
• Protein extraction techniques; Salting precipitation with Amonium Sulfate,
Histone-Like Proteins extraction by John’s method using PCA
• Western hybridization analysis using SDS PAGE
• Keeping proper and detailed records of day to day lab procedures and experiments
• Keeping to standard operation procedures (SOPs)
Volunteer
work
experience
May 2010 – July 2010
Ocular Developmental Laboratory
University of Melbourne
Currently learning and working with techniques such as:
• DNA and RNA extraction from mouse tissue
• Use of microtome for slide preparation from paraffin-embedded retina tissue
• Mouse Genotyping using PCR
May 2010 – July 2010
Genomics and Systems Biology Laboratory
DNA & Blood Profiling Facility
Baker IDI Heart and Diabetes Institute
Currently learning and working with techniques such as:
• DNA & RNA quantification and assessment using Nanodrop and Qubit
• Gene Expression Microarray using illumina BeadStudio platform
Publications
(2)
Assaying the presence of histone-like protein HU in Halobacillus karajensis.
Ghadam P, Shariatian N, Amoozegar MA, Rabbani A, Shahriari SH, Pakistan Journal of
Biological Sciences 10 (19): 3248-3253, 2007
“Nold, M.F., N.E. Mangan, I. Rudloff, S.X. Cho, N. Shariatian, T.D. Samarasinghe, E.M.
Skuza, J. Pedersen, A. Veldman, P.J. Berger, and C.A. Nold-Petry, Interleukin-1
receptor antagonist prevents murine bronchopulmonary dysplasia induced by
perinatal inflammation and hyperoxia. Proceedings of the National Academy of
Sciences of the United States of America, 2013. 110(35): p. 14384-9.”
Posters (2)
• Assaying the Presence of Some Histone-Like Proteins (for example HU) in
Halobacillus karajensis; Parinaz Ghadam, Nikeh Shariatian, Azra Rabbani and
Mohammad Ali Amoozegar
Published in Journal of Peptide Science, Volume 12 Issue S1 - August 2006 (81-
243) Supplement: 29th European Peptide Symposium, September 2006, Gdansk,

3. Poland www.Interscience.wiley.com
• Assaying the presence of H-NS protein in a native strain of Halomonas species;
Parinaz Ghadam,Shadab Shahriari, Mohammad Reza Soudi, Ashraf Shabani and
Nikeh Shariatian
Published in Journal of Peptide Science, Volume 12 Issue S1 - August 2006 (81-
243) Supplement: 29th European Peptide Symposium, September 3 - 8, 2006,
Gdansk, Poland www.Interscience.wiley.com
• The Lethal Impact Of Prenatal Maternal Nicotine And Inflammation On Newborn
Mice; Thilini Samarasinghe, Marcel F Nold, Ina Rudloff, Elizabeth M Skuza, Nikeh
Shariatian, Mandar S Joshi, Philip J Berger, Claudia A Nold-Petry
Presented at the Lorne Infection and Immunity Conference 2014
Languages
• English (fluent)
• Farsi/Persian (fluent)
IT Skills
• Proficient in Microsoft Office Programs (Microsoft Word, Excel, Access, Power
point)
• Proficient in Biological softwares (Clustal X, Biochem, DNA man, Net primer, Primer
3)
Personal
Attributes
• Good knowledge of Molecular Biology
• Quick learner
• Hardworking and dedicated
• Persistent
• Team player
• Ability to work independently
• Excellent organizational skills
• Good communication skills
Referees
• Associate Professor Tim Moss
• Dr. Rob Bischof