1. Datasheet for TP22 & TP38 prevents it from falling out and facilitates the
alignment of the pin.
The pin aspirates liquid from a well/microtiter
plate by dipping the tip herein. Capillary forces
draw liquid into the slot and into a closed cavity
inside the pin. The closed cavity prevents
evaporation of the liquid. The pin attached in the
robot is then moved over the substrate and
dispenses liquid when the tip touches the surface
of the substrate. A single uptake can print up to
10.000 spots, and the TP22 pin prints uniformly
TP22_10 and TP22_50 homogenous, circular quality from the first to the
last spot.
The TOPS-PIN TP22 represents a new
generation in microspotting pins providing Please contact us if special versions with
unrivalled benefits to the customers such as: downscaled spot size are required for medium to
high density arrays.
• Reduced assay time
• More reliable results Dimensions
• Competitive pricing
• Plug & play
• Customisable pins
The TOPS-PIN TP22 is designed and
manufactured with a combination of silicon and
stainless steel materials. This enables the TP22
to combine the durability of the silicon material
with the “plug and play” features build in by the
steel guide.
The TOPS-PIN TP22 can be used in any existing
microarray robot on the market. The TP22 is
directly interchangeable with existing
microspotting pins and do not require
modifications to the pin holder or robot. Quality control
Applications The TOPS-pin Silicon pins are manufactured with
The TOPS-PIN TP22 is able to deposit sub- strict quality control involving infrared camera
nanoliter amounts of liquid onto surfaces to inspection verifying the quality of the bonding and
generate microarrays. validation in laboratory to verify performance
before pins are shipped.
The pin is specifically suited for microarrays,
DNA-arrays and protein arrays. The pin can be Ordering information
used for deposition of organic material like cells, When ordering please specify type and length.
virus, DNA, RNA, proteins, peptides etc. Orders can be made through the homepage
www.topspin.dk or direct at the address given
Description below:
The main pin is made in silicon that doesn’t wear
out as traditional steel or tungsten pins. The TOPS-PIN
TP22 comes with a tapered tip and a slot in one Oersteds Plads, Build. 345 east
end. DK-2800 Lyngby
Denmark
The TOPS-PIN TP22 is 22.3 mm but will in Tel: (+45) 45 25 58 28
December 2004 also be available in a 38.2 mm Mob: (+45) 23 82 38 36
long version (TP38). A collar in the end in Fax: (+45) 45 88 77 62
combination with a steel guide ensures precisely Email: info@topspin.dk
control of three-dimensional movement. The pin
moves almost frictionless in the holder in a
vertical position with the tip down, the collar

2. List of TOPS-PIN Products:
Product no. Pin Length Tip size Spot Dispensed Sample Number of
[mm] [µm] diameter volume volume [µl] spots per
[µm] [nl] loading
1 15.000
TP22_10 22.3 20 ~ 60 0.65 0.6 9.000
0.2 3.000
1 16500
TP22_50 22.3 60 ~100 0.90 0.6 6600
0.2 3300
1 15.000
TP38_10 38.2 20 ~ 60 0.65 0.6 9.000
0.2 3.000
1 16500
TP38_50 38.2 60 ~100 0.90 0.6 6600
0.2 3300
*Spotsize and dispensed volume is also dependent on printing parameters and type of slide applied.
Contact:
TOPS-PIN
Oersteds Plads, Build. 345 east
DK-2800 Lyngby
Denmark
Tel: (+45) 45 25 58 28
Mob: (+45) 23 82 38 36
Fax: (+45) 45 88 77 62
Email: info@topspin.dk
Homepage: www.topspin.dk