Nexterion Spot is a twofold concentrated spotting solution that can be modified to create different solutions by adding detergents. Nexterion Spot I is identical to the base Nexterion Spot solution, while Nexterion Spot II and III are prepared by adding cetyltrimethylammoniumbromide or Triton X-100 respectively to Nexterion Spot in concentrations of 0.02%. Nexterion Spot AM I contains 6xSSC with 3 M betaine instead of Nexterion Spot, and Nexterion Spot AM II is prepared by adding betaine to Nexterion Spot. The detergent concentration can be varied between 0.01-0.1% for modifying spot size.