1. PRODUCTION OF INSULIN
FROM MICROBIAL
CULTURE
RUI BAI
MUHAMMAD AKLIL BIN AYOB
YANGJIE CUI
CHEN 60351 : CORE
BIOTECHNOLOGY 1

2. CONTENT
1.
2.
3.
4.
5.
Abstract
Introduction
Main process of insulin production
Significance of human insulin
Conclusion

3. Abstract




Hormone secreted by
pancreas
Control blood sugar level
Lack of insulin caused
diabetes mellitus
Industrial production by
manipulating genetic
engineering and microbial

4. Introduction



Protein generated
by beta cell of
pancreas (islet of
Langerhans)
Exist in animal:
porcine and bovine
Build up of 51 amino
acids



30 amino acids form
chain B
21 amino acids form
chain A
2 chain linked by
disulfide bonds

5. (Introduction)…cont
www.bio.davidson.edu
www.depositphotos.com

6. (Introduction)…cont


Diabetes mellitus
(DM) lack of insulin
to control blood
sugar level
Need to insulin
therapy to keep
healthy
www.som.uq.edu.au

7. Main process of insulin production
Microorganisms
Fungi
Yeast

8. Microorganisms
Kinds of bacteria
E. coli
www.google.com.hk

9. Genetic Engineering
http://blogs.swa-

10. Microbial Fermentation

11. Significance of human insulin


1978: insulin from animal; pig and cow
Switch to microbial culture. Why?

Quantity & quality is better


E. coli culture is convenient


No allergy and side effect
Cheaper for commercial
Predictable

Problems easily solved

12. Conclusion




Insulin market grown
at 7% annual rate
Company: Novo
Nordisk, Eli Lilly &
Sanofi Aventis
Cost : £75 – 200 per
vial
Different delivery ways
www.novonordisk.com

13. Thank you!
http://www.bd.com