The TRP53 gene contains 11 exons and 10 introns. Exon 1 is 128 base pairs and exon 11 is 523 base pairs, which is the longest exon. The introns range in size from 5 base pairs to 793 base pairs. The gene is located on chromosome 11 in humans and encodes a tumor protein involved in regulating the cell cycle and suppressing tumor formation.

1. Allegato 2
Schema della struttura del gene TRP53
Legenda:
Regione esonica non tradotta
Regione esonica tradotta
Regione intronica
Struttura del gene Trp53 (Mus Musculus)
5' ..........gggggttgctgggattgggactttcccctcccacgtgctcaccctgg
downstream cta
sequence
Exon Lunghezza(nt)
1 128 AAGTTCTGTAGCTTCAGTTCATTGGGACCATCCTGGCTGTAGGTAGCGACTACAGTTAGG
GGGCACCTAGCATTCAGGCCCTCATCCTCCTCCTTCCCAGCAGGGTGTCACGCTTCTCCG
AAGACTGG
Intron 6,37 gtaagtaattgatgagcgtgacgagacctctcggtcactggctctctccgtttgcatcca
1-2 5 taaaactagagaaaaccgtggggtttgggggtggggcagt....................
....................attctaccctttcctataagccataggggtttgtttgttt
gtatgttttttaattgacaagttatgcatccatacagtacacaatctcttctctctacag
2 83 ATGACTGCCATGGAGGAGTCACAGTCGGATATCAGCCTCGAGCTCCCTCTGAGCCAGGAG
ACATTTTCAGGCTTATGGAAACT
Intron 283 gtgagtggatctttttggggcccttaagatacatcccgccatacctgtatcctccccttg
2-3 cctgagagaaacaaaaacagtagtgttcaaacatggtatg....................
....................tgactacatagcaagttggaggccagcctgggataagtga
gattctgtcttcaaaaaatggaaggaaatcaggaactaactctctgctcttgttttccag
3 22 ACTTCCTCCAGAAGATATCCTG
Intron 93 gtaaggcccagagcagaaagggacttgggctttggtgttgggtggtaggctgagaacac
3-4 agtcctgagggttcttctttgtcccatccacag
4 261 CCATCACCTCACTGCATGGACGATCTGTTGCTGCCCCAGGATGTTGAGGAGTTTTTTGAA
GGCCCAAGTGAAGCCCTCCGAGTGTCAGGAGCTCCTGCAGCACAGGACCCTGTCACCGAG
ACCCCTGGGCCAGTGGCCCCTGCCCCAGCCACTCCATGGCCCCTGTCATCTTTTGTCCCT
TCTCAAAAAACTTACCAGGGCAACTATGGCTTCCACCTGGGCTTCCTGCAGTCTGGGACA
GCCAAGTCTGTTATGTGCACG
Intron 731 gtgagtgggccccggggagttgtctttcgtgtgacctttcaatgcctctttttctctgtc
4-5 tagatttgggggttcctcttcagcctgtagactgtgcctc....................
....................tccaatggtgcttggacaatgtgtttcattagttccccac
cttgacacctgatcgttactcggcttgtccccgacctccgttctctctcctctcttccag
5 184 TACTCTCCTCCCCTCAATAAGCTATTCTGCCAGCTGGCGAAGACGTGCCCTGTGCAGTTG
TGGGTCAGCGCCACACCTCCAGCTGGGAGCCGTGTCCGCGCCATGGCCATCTACAAGAAG
TCACAGCACATGACGGAGGTCGTGAGACGCTGCCCCCACCATGAGCGCTGCTCCGATGGT
GATG
Intron 78 gtaagccctcaacaccgcctgtggggttaggactggcagcctcccatctcccggcttctg
5-6 acttattcttgctcttag
6 113 GCCTGGCTCCTCCCCAGCATCTTATCCGGGTGGAAGGAAATTTGTATCCCGAGTATCTGG
USR – IFOM – ANSAS
“LO STUDENTE RICERCATORE - Anno 2010 – Relazione finale scientifica dello studente

2. Allegato 2
AAGACAGGCAGACTTTTCGCCACAGCGTGGTGGTACCTTATGAGCCACCCGAG
Intron 402 gtctgtaattttgttttggtttgtgcgtcttagagacagttgactccagcctagactgat
6-7 gttgactttctagcaacccgtttgcctcaccctcctgagt....................
....................tgaaacttattagaggctatagccagccattcccggctgc
tgcaggtcacctgtagtgaggtagggagcgacttcacctggatcctgtgtcttcccccag
7 110 GCCGGCTCTGAGTATACCACCATCCACTACAAGTACATGTGTAATAGCTCCTGCATGGGG
GGCATGAACCGCCGACCTATCCTTACCATCATCACACTGGAAGACTCCAG
Intron 322 gtaggaaggcgcgtggtaggttaggttagcctgtttcttccccagcttctgcctgtttct
7-8 gttccacgagtcccgccccctaccacatgcccaacgctct....................
....................aagaggggttgggaacaggtgggggcctagtttacacaca
gtcaggatggggcccagctttcttactgccttgtgctggtccttttcttgtcccggatag
8 137 TGGGAACCTTCTGGGACGGGACAGCTTTGAGGTTCGTGTTTGTGCCTGCCCTGGGAGAGA
CCGCCGTACAGAAGAAGAAAATTTCCGCAAAAAGGAAGTCCTTTGCCCTGAACTGCCCCC
AGGGAGCGCAAAGAGAG
Intron 79 gtacgcaggcgggaccaaggaggcggaggagcctgttgagcttcaccccaaagtcacctc
8-9 ttgctctctccttccacag
9 74 CGCTGCCCACCTGCACAAGCGCCTCTCCCCCGCAAAAGAAAAAACCACTTGATGGAGAGT
ATTTCACCCTCAAG
Intron 793 gtaccaaggctggagagtcgcatgccagagacaagctgtacccattattgcctctgtctc
9-10 tcgcatgtataaaatagtgttattagcaggttgccaggtc....................
....................ttaagttgggaaccaactttcagaaagaaagttgttaaaa
tcgtgaaagtggttgtgtgaccttgtccagtgcttccatctcacttcatctctgctgcag
10 107 ATCCGCGGGCGTAAACGCTTCGAGATGTTCCGGGAGCTGAATGAGGCCTTAGAGTTAAAG
GATGCCCATGCTACAGAGGAGTCTGGAGACAGCAGGGCTCACTCCAG
Intron 587 gtaagtggcctggggcagcgcctgcctgtggtgctctacccagacctccctccagctcag
10-11 cctttgtagtgaaagataaaaaccccaccctgctagatgc....................
....................tcagcctccagcctagagccttccaagccttgatcaagga
ggaaagcccaaactgctagctcccatcacttcatccctccccttttctgtcttcctatag
11 523 CTACCTGAAGACCAAGAAGGGCCAGTCTACTTCCCGCCATAAAAAAACAATGGTCAAGAA
AGTGGGGCCTGACTCAGACTGACTGCCTCTGCATCCCGTCCCCATCACCAGCCTCCCCCT
CTCCTTGCTGTCTTATGACTTCAGGGCTGAGACACAATCCTCCCGGTCCCTTCTGCTGCC
TTTTTTACCTTGTAGCTAGGGCTCAGCCCCCTCTCTGAGTAGTGGTTCCTGGCCCAAGTT
GGGGAATAGGTTGATAGTTGTCAGGTCTCTGCTGGCCCAGCGAAATTCTATCCAGCCAGT
TGTTGGACCCTGGCACCTACAATGAAATCTCACCCTACCCCACACCCTGTAAGATTCTAT
CTTGGGCCCTCATAGGGTCCATATCCTCCAGGGCCTACTTTCCTTCCATTCTGCAAAGCC
TGTCTGCATTTATCCACCCCCCACCCTGTCTCCCTCTTTTTTTTTTTTTTACCCCTTTTT
ATATATCAATTTCCTATTTTACAATAAAATTTTGTTATCACTT
3' atatggttttgagaggttgatatcagcataagctgtctgggcccccaggg..........
downstream
sequence
Le sequenze in minuscolo verde corrispondono alle regioni non tradotte alle
estremità del gene (UTRs)
Le sequenze in minuscolo blu corrispondono alle regioni introniche situate tra gli
esoni
Le sequenze in maiuscolo nero corrispondono alle regioni esoniche tradotte
Le sequenze in maiuscolo viola corrispondono alle regioni esoniche non tradotte
La sequenza codificante inizia con lo start codon ATG (Met) e termina con lo stop
codon TGA
USR – IFOM – ANSAS
“LO STUDENTE RICERCATORE - Anno 2010 – Relazione finale scientifica dello studente