DEAE-Dextran method

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2. DEAE-DEXTRAN
METHOD

3. Content
DEAE-Dextran
Principle
DEAE-dextran method
Example “mammalian cultured cells”
Advantages and disadvantages
Conclusion
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5. DEAE-DEXTRAN
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7. STEPS of DEAE-DEXTRAN
Step:1 Step:2 Step:3
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Step:4
Step:5

8. STEP NO: 1
MIX
NUCLEIC
ACID WITH
DEAE
• Mix nucleic acid with DEAE-
dextran solution in transfection
medium or phosphate-buffered
saline solution.
• Nucleic acid-DEAE-dextran
complexes are formed via
electrostatic interactions between
the polymer and phosphate
backbone of the nucleic acid.
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9. STEP NO: 2
ADD THE
NUCLEIC
ACID DEAE
Add the nucleic acid-
DEAE-dextran
complexes to the cells,
which adhere to the cell
surface via
electrostactic
interactions.
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10. STEP NO: 3
INDUCE
THE
UPTAKE
• Induce the uptake of
the nucleic acid-DEAE-
dextran complexes by
osmotic shock using
DMSO or glycerol.
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11. STEP NO: 4
WASH
CELLS
Wash cells to remove
the complexes and
incubate to allow
gene expression.
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12. STEP NO: 5
ASSAY
CELLS
Assay cells for
transient gene
expression.
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15. ADVANTAGES DISADVANTAGES
 Low Cost
 Easy Technique
 Quick process
 Can be used for wide
variety of cells
High conc of DEAE
dextran can be toxic
Efficiency of transfection is
low
Uptake of genes is also
less compared to other
techniques
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16. CONCLUSION
 DIETHYLAMINOETHYL – dextran Cationic Nature (+)
 DEAE + DNA +ive
 Electrostatic interaction / Cytoplasm Cell transformed
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