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To elucidate the mechanism of bromodeoxyuridine (BrdU) induced cellular senescence, we treated HeLa cells with D4476, a potent and specific inhibitor of casein kinase 1(CK1). We found that D4476 (10µM) treatment could arrest cell growth at G1 stage and induced cellular senescence when treated together with BrdU (10µM). However neither D4476 nor BrdU can induce cellular senescence alone, at a concentration of 10µM. These results suggest that the targets of CK1 may be involved in maintaining normal cellular process and their inactivation potentiates BrdU to induce senescence like phenomena.

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