1. RECENT DEVELOPMENTS IN HALAL FOOD ANALYSIS YAAKOB B. CHE MAN, Ph.D INSTITUTE OF HALAL FOOD Universiti Putra Malaysia In conjuction with the Annual General Meeting of MIFT, 20 May 2006, Subang Jaya, Selangor
7. Al Quran Surah Al-Baqarah: “ O ye people! Eat of what is on earth, Halal and good ; and do not follow the footsteps of the Evil One, for he is to you an avowed enemy.” (Verse 168)
8. Al-Quran Surah Al-Maidah “ O ye who believe! Forbid not the good thing which Allah hath made Halal for you, and transgress not. Lo Allah loveth not transgressors. Eat of that which Allah hath bestowed on you as food Halal and Good , and keep your duty to Allah in Whom ye are believers.” (verse 87 –88)
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11. Halal Food Production - From Farm to Table Farm Raw material: *Animal *Plant Processing Handling e.g. slaughtering Processing/Unit Operations *Preliminary operation *Conversion operation *Preservation operation *Product development Ingredient & Additive Handling Packaging Storage Transportation Storage & Distribution Consumption
50. M A1 A2 A3 D1 D2 D3 K1 K2 P1 P2 228 bp 131 bp 500 bp ≈ 360 bp Restriction Enzyme Analysis of PCR Amplified Cytochrome b Gene (sausages) M-1Kb DNA ladder A1, A2 and A3- sosej ayam berlainan jenama D1, D2 and D3- sosej daging lembu berlainan jenama K1 and K2- sosej babi berlainan jenama P1 and P2- sosej yang tidak dikenalpasti
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52. 1 2 3 4 5 A B C D E F G H ELISA Results A1- positive control; B1 and C1- negative controls; D1- mutton, E1- beef; F1- chicken meat, G1, H1, A2 and B2-pork; C2- mutton fat; D2- beef fat; E2- chicken fat; F2, G2, H2, A3- lard; B3, C3 and D3- chicken sausages with different brands; E3, F3 and G3- beef sausages with different brands; H3 and A4- pork sausages with different brands; B4 and C4- unknown sausages; D4, E4 and F4- unknown casings; G4, H4, A5 and B5- bread with different brands; C5 and D5- biscuits with different brands; E5- homemade biscuit with 1% lard; F5- homemade biscuit with 50% lard; G5- homemade biscuits with 100% lard.