1. ADVANCING CANADIAN AGRICULTURE AND AGRI-FOOD
PROJECT # AB0159CO
‘‘Testing of Photocatalytic
Technologies for Use on Food Surfaces:
Use of Natural Products as
Photocatalytic Agents’’
Nick D. Allan, M.Sc.
Contract Research Manager

2. Biofilm Primer

3. The Problem
The Need for Hard/Food Surface
Sanitizers
 CFIA’s food safety yearly spending is CAD$360M1
 The National Cattlemen’s Beef Association
estimates $1B in beef sales are lost each year in
the United States to spoilage1
 The Canadian Cattleman’s Association estimates
that $ 200M in sales are lost each year2.
1)Food safety Report of the US Government Accountability Office, 2005
2)The Cattlemen’s news Letter. Vol. 6, 2005.

4. The Problem
Barriers to Developing a Good Hard
Surface Sanitizer
 Effectiveness
 Biofilms (ACAAF project # AB00008)
 Concentration
 Interference/Inactivation by environment
 Duration of Efficacy
 Human/Food Safety

5. The Solution: Photo Dynamic Therapy
Contaminating Biofilm

6. The Solution: Photo Dynamic Therapy
Light Source
1O
2
Innovotech compound

7. The Solution: Photo Dynamic Therapy
1O 1O bactericidal
2 2
effect
1O
2

8. Project Overview:
The Development of Sani-LuxTM
 PHASE I: Food Dye Screening
 Physical Properties
 Biological activities
 PHASE II: Hard Surface and Food Surface
Screening (Practical Testing)
 PHASE III: Filing as a Sanitizer with CFIA

9. PHASE I:
Food Dye Screening
 Evaluation of Most known food dyes
 Solubility (water, olive oil, sorbitol, PEG, Glycerin)
 Biological Activities
 Salmonella
 Staphylococcus aureus
 E. coli O157:H7
 Listeria monocytogenes
 Concentration range
 Light Intensity
 Contact Time

10. PHASE II:
Food and Hard Surface Screening
~2.0 Log ~1.0 Log
 Evaluation against E. coli and Listeria
monocytogenes
 Concentration range @ 16 hours
 Variation in effectiveness

11. PHASE II:
Food and Hard Surface Screening
 Evaluation against
Salmonella and
Listeria
monocytogenes
 Concentration range
@ 16 hours
 Variation in
effectiveness
 3.8 Log reduction for
Listeria
 2.4 Log Reduction for
Salmonella

12. PHASE II:
Food and Hard Surface Screening
 Hard Surface Evaluation against E. coli
 40 ppm @ 3.5 hours
 4.5 Log Reduction

13. PHASE III:
Filing with CFIA
 Registration Package:
 AOAC Carrier Testing
 Log10 Reduction Testing
 Label Claim Organisms (Salmonella, S. aureus, E. coli
O157:H7, Listeria monocytogenes, Campylobacter jejuni)
 Must Demonstrate 95% killing (~1.5 Log Reduction)
 40 ppm under light intensity of 680-780 lumens using
Lithonia Lighting at 24±2 °C for 3.5 hours
 COA
 Sample Label
 IFU

14. PHASE III:
Sample Label

15. PHASE III:
IFU

16. Sani-LuxTM Summary
 Broad spectrum kill: Listeria, Staphylococcus,
Campylobacter, E. coli and Salmonella, as well as
fungal pathogens such as Fusarium, Aspergillus,
Pythium, and Penicillium species.
 Effective on a variety of surfaces: can be used on
stainless steel, ceramic, plastic, concrete, or granite.
 Non-corrosive to metals encountered in household,
beverage and food processing applications.
 Non-toxic (Approved for use in foods at 100X the active
concentration).
 GREEN: contains only natural products.
 Natural Deodorizer: Leaves cleaned areas smelling
fresh.
 Continually active when a light source is present.