1. ANTISPERM ANTIBODY
ASSOCIATED WITH
INFERTILITY IN MEN

2.  Infertility is defined as inability of couples to achieve
pregnancy following one year of unprotected intercourse.
 Primary infertility
 Secondary infertility

3.  Male infertility may be due to:
1. Decrease in the number of sperms
2. Sperm being blocked from being released
3. Sperm that do not work properly
 Other causes of male infertility…..?

4.  Semen analysis…………………….male pathology.
 Techniques for andrology for monitoring semen
parameters, like sperm concentration, motility and
morphology.
 Computer assisted semen analysis (CASA)

5.  CASA has categorized motility
(micrometer/sec), of sperm into three classes
as:
1. average path velocity (VAP)
2. straight line velocity (VSL)
3. curvilinear velocity (VCL)

6.  Testicular pathology is divided in to subgroups as:
1. Teratozoospermia
2. Oligozoospermia
3. Asthenozoospermia
4. Azoospermia
5. Polyzoospermia

7.  Antisperm antibodies are antibodies directed against the
sperm.
 Research began in 1899
 Found systematically and in local secretion.
 Impair the fertilizing ability of spermatozoa.

8.  To compare the data of semen parameters from fertile
and infertile subject categories.
 To see the role of antisperm antibodies on sperm
motility analyzed through CASA in fertile and fertile
male subjects.
 To determine the impact of ASA in our population and
how it varies in different categories of infertile male
subjects.
 To determine whether seminal plasma ASA can be used
as diagnostic tool in evaluating male fertility potential.

9. (n=10) fertile and (n=72) infertile male
subjects.
The infertile patients were subdivided in
to different categories :
(ATZS), (AZS), (OZS), (OAZS), (SA).
Semen volume, sperm count, CASA,
motility parameters were measured
according to WHO criteria.
Antisperm antibody enzyme linked
immunosorbent assay (ELISA) kit.

11.  There was non-significant (P>0.05) difference in mean ± SEM
semen volume among fertile and different categories of
infertile male subjects (ATZS, OZS, OAZS, SA).
 However azoospermics (AZS) patients showed significant
(P<0.01) decreased in semen volume as compared to fertile
men.

12. a**
0
1
2
3
4
5
6
fertile ATZS AZS OAZS OZS SA
Semenvolume(ml)
Subjects categories
fertile
ATZS
AZS
OAZS
OZS
SA
a**
Figure 1: Mean ± SEM semen volume (ml) of fertile (n=10) and
infertile (n=72) astheno-teratozoospermics (ATZS),
azoospermics (AZS), oligoasthenozoospermics (OAZS)
oligozoospermics (OZS) and sperm agglutination (SA) male
subjects.

13.  There was highly significant (P<0.001) decrease in sperm
count OZS, OAZS as compared to fertile male subjects.
 While patients with sperm agglutination showed highly
significant (P<0.001) increase in sperm count when
compared with fertile.
 There were non significant difference (P>0.05) in mean ±
SEM sperm count (mill/ml) among fertile and ATZS infertile
male subjects.

14. Figure :Mean ± SEM sperm count (mill/ml) of fertile (n=10) and
infertile (n=72) astheno-teratozoospermics (ATZS),
azoospermics (AZS), oligoasthenozoospermics (OAZS),
oligozoospermics (OZS) and sperm agglutination (SA) male
subjects.
0
50
100
150
200
250
Fertile ATZS AZS OAZS OZS S.A
Spermcount(mill/ml)
Subjects categories
Fertile
ATZS
AZS
OAZS
OZS
S.A

15.  There was significant (P<0.05) difference in mean ± SEM
sperm motility (%) among fertile and different categories of
infertile male subjects.

16. Mean ± SEM sperm motility (%) in different classes of sperm motility (rapid
progressive motile (%), slow progressive motile (%), and non motile (%) of fertile
(n=10) and infertile (n=72) astheno-teratozoospermics (ATZS), azoospermics (AZS),
oligoasthenozoospermics (OAZS), oligozoospermics (OZS) and sperm
agglutination (SA) male subjects.
0
10
20
30
40
50
60
Fertile ATZS OAZS OZS AZS SA
Spermmotility(%)
Subjects categories

17.  Curvilinear velocity (VSL µm/sec)
There was non-significant (P>0.05) difference in mean ± SEM
curvilinear velocity (VSL µm/sec among fertile and different
categories of infertile male subjects.
 Straight line velocity (VSL µm/sec)
There was significant (P<0.05) difference in mean ± SEM
straight line velocity (VSL µm/sec) among fertile and
different categories of infertile male subjects.

18.  Average path velocity (VAP µm/sec)
There was non significant (P>0.05) difference in mean ± SEM
Average path velocity µm/sec (VAP µm/sec) among fertile
and different categories of infertile male subjects.
 Wobble (WOB µm/sec)
There was significant (P<0.05) difference in mean ± SEM
average path WOB among fertile and different categories of
infertile male subjects.

19.  Amplitude of lateral head (ALH µm/sec)
There was significant (P<0.05) difference in mean ± SEM
amplitude of lateral head µm/sec (ALH) among fertile and
different categories of infertile male subjects.

20.  ASAs had showed highly significant (P<0.001) increased in
concentration in sperm agglutination (SA) infertile male
subjects and asthenoteratozoospermics (ATZS) patients
showed significant (P<0.05) increased in ASAs as compared
to oligozoospermics (OZS), oligoasthenozoospermics
(OAZS) and azoospermics (AZS) infertile male subjects.

21. Mean ± SEM antisperm antibodies (U/ml) of fertile (n=10) and infertile
(n=72) astheno-teratozoospermics (ATZS), azoospermics (AZS),
oligoasthenozoospermics (OAZS), oligozoospermics (OZS) and sperm
agglutination (SA) male subjects.
0
20
40
60
80
100
120
Fertile ATZS OAZS OZS AZS SA
ASA(U/ml)
Subjects categories

22.  Standard semen parameters have prognostic ability to
evaluate the male fertility.
 Seminal plasma antisperm antibodies (ASAs) showed
significant increased in concentration in different
categories of infertile male’s subjects.
 Seminal plasma antisperm antibodies (ASAs) can be
used as diagnostic tool for evaluation of male infertility
especially in those infertile patients having sperm
agglutination (SA) and asthenoteratozoospermics
infertile patients (ATZS).