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AS 252 PRINCIPLES OF ANIMAL
NUTRITION
BY
FRANK IDAN, PH.D.
LECTURER
DEPARTMENT OF ANIMAL SCIENCE, KNUST
EMAIL: frank.idan@knust.edu.gh/frankkidan@gmail.com
MOB: 0243389831
BACKGROUND
2
 Teaching Philosophy
Every student has the potential to excel when given the
right opportunities and guidance
Students need to stay focus, work hard, and be committed
to their academic work
LECTURE OUTLINE
3
 What is Nutrition and Nutrient?
 Nutritional Requirements
 Components of Plants and Animals
 Six Classes of Nutrients and Functions of Nutrients
Water, CHO, Protein, Lipids, Vitamins and Minerals
THE SCIENCE OF NUTRITION
 What is Nutrition?
 The science of nutrition examines the qualitative and quantitative
requirements of the diet necessary to maintain proper health of
animals
 Involves various chemical reactions and physiological processes which
transform food into body tissues and activities
 Qualitative Requirements: Essential nutrients
 Quantitative Requirements: Balanced diet – correct nutrients, in proper
amounts, that is required by animals to stay healthy
4
DEFINITIONS AND TERMS
 Nutrients: Any chemical element or compound in the diet that is
required for normal life processes
 Components of food capable of being utilised by animals
 Presently, more than 100 nutrients are needed in animal diets
 Food: An edible material that provides nutrients
 Material which, after ingestion by animals, is capable of being digested,
absorbed, and utilised
 Animals categorized into Herbivores, Carnivores and Omnivores
depending on the type of food
 Feed: commonly used to designate animal food
5
DEFINITIONS AND TERMS
 Diets: A mixture of feedstuffs used to supply nutrients to an animal
 Ration: An amount of feed allocated to an animal in a day i.e. how
much feed an animal gets per day
 Feed: commonly used to designate animal food
 Foodstuff/Feedstuff: Any material made into or used as food for
animals
6
NUTRITIONAL REQUIREMENTS
 Plants vs Animals: Both plants and animals require Water and Energy
 Autotrophic: Type of nutrition in which organisms synthesize their own food from simple
inorganic materials such as H2O, CO2 and mineral salts in the presence of sunlight
 Plants and other chlorophyll containing organisms
 Process called Photosynthesis which is the opposite of respiration in Animals
 6 CO2 + 6H2O C6H12O6 + 6 O2
 Heterotrophic: Mode of nutrition in which organisms depend on other organisms for
their food to survive
 Cannot manufacture own food
 Mostly animals, fungi, and other non-photosynthetic organisms
7
6CO2 + 6H2O C6H12O6
Sunlight
Chlorophyll
NUTRITIONAL REQUIREMENTS
 Essential Nutrients for Most Animals:
 Water
 Energy* = Carbohydrates, Lipids and Proteins
 Amino Acids (Nitrogen sources)
 Fatty Acids
 Minerals = Macro and Micro
 Vitamins = Fat and Water soluble
 Other Nutrients (Species dependent) – Taurine, Choline, Carnitine etc.
8
6CO2 + 6H2O C6H12O6
FACTORS AFFECTING NUTRITIONAL
REQUIREMENTS
 Gastrointestinal Tracts of Species
Carnivores, Herbivores, and Omnivores
Energy sources
Carnivores/Omnivores – Fat/Protein
Herbivores - Carbohydrates
 Age
 Level of Productivity
 Production, Gestation, and Lactation
 Environmental Factors
 Temperature, Humidity
9
6CO2 + 6H2O C6H12O6
COMPOSITION OF ANIMAL FEED
10
6CO2 + 6H2O C6H12O6
What is actually contained in Animal Feed?
1. Moisture (Water)
2. Dry Matter
a. Organic Material: (N-containing compounds (proteins), lipids (fat
soluble), carbohydrates (water soluble), vitamins, nucleic acids, and
organic acids
b. Inorganic Material (Ash): Minerals Macro and micro/trace minerals;
Any mineral can be toxic in excessive amounts
COMPOSITION OF ANIMAL FEED
11
6CO2 + 6H2O C6H12O6
Plants and animals contain similar types of chemical substances, can be
grouped into classes according to constitution, properties and function
COMPOSITION OF PLANTS AND ANIMALS
 Plants – Mostly water and carbohydrates
CHO- Readily available (sugars) vs fibrous components
(crude fibre)
Low in protein and fat
 Animals – Mostly fat and protein
60% water, 20% fat, 16% protein, and 4% minerals
Very low in carbohydrates (< 1%)
12
THE CHEMICAL COMPOSITION OF PLANTS
AND ANIMALS BODY
 Physical and chemical processes occurring in the protoplasm
represents the chemical composition of plant and animal
 Animal body derives all nutrients for its physiological functions from
the digestion of plant and plant products as well as animal products
such as fish meal and milk
 Knowledge of the chemical composition of farm animals is required to
understand their nutrient requirement while that of plants is essential
because they furnish most of the food for livestock
13
THE CHEMICAL COMPOSITION OF PLANTS
AND ANIMALS BODY CONT’D
 Plants and animals' tissues are composed of similar type of chemical
substances, but their relative amounts vary
 Plants are analyzed by proximate method of analysis whereas, animal
body was first analyzed by Lawes and Gilbert (1858)by slaughter
experiments
 Plants and their by-products show much larger differences in the
chemical composition than the animals
 Proportions of various body organs of animals change during the
period before and immediately after birth and during post-natal
growth
14
COMPARISON OF PLANTS AND ANIMALS
BODY COMPOSITION
Parameters Animal Plant
Major constituent Water Water
Major Organic Constituent Protein Carbohydrate (CHO)
Structural Component Protein and Mineral CHO (Cellulose, Lignin
Hemicellulose etc.)
Reserve Material Fat (Glycogen) CHO (Starch)
CHO Amount Less More
Minerals Amount Generally constant to species Wide variation
Variations in Composition Less Wide
15
Table 1: Chemical Composition of The Pig’s
Body During Early Post-natal Life
16
Post Natal Period (days)
0 7 14 28
Empty BW (g) 1450 3044 5248 9651
Lipid (%) 1.2 10.1 15.1 18.3
Protein (%) 12.0 14.4 14.4 14.6
Ash (%) 4.2 3.1 3.0 3.3
Water (%) 82.6 72.5 67.3 63.6
SIX CLASSES OF NUTRIENTS
 Water
 Carbohydrates (CHO)
 Lipids (Fats & Oils)
 Proteins (CHON)
 Minerals
 Vitamins
17
FUNCTIONS OF NUTRIENTS
1. Source of Energy: CHO, lipids and proteins
 Energy needed for heat production, fat deposition, and work
2. Maintenance: All nutrients
 Nutrients are needed for breathing, movement, digestion, maintaining body
weight, keeping warm etc.
3. Growth: Proteins
 Most rapid at early stages of life
 Increase in height, weight, building of tissues
4. Reproduction: All nutrients
 Development of maternal tissues and foetus
18
FUNCTIONS OF NUTRIENTS CONT’D
5. Structural: Proteins, minerals, fats, and water
 Maintenance of structural Integrity
 Building/maintaining body structure, cushioning of body organs e.g., Heart
6. Regulatory: Vitamins, minerals, protein, and water
 Transport of nutrients, stimulating or catalyzing activities of hormones or
enzymes needed for particular metabolic processes
7. Production: All essential nutrients
 Meat, Milk, and Egg production
19
FACTORS AFFECTING NUTRIENT
REQUIREMENTS
1. Species
2. Breeds
3. Weight
4. Body condition
5. Growth
6. Stage of gestation
7. Level of milk production
8. Age
9. Sex
10. Work
11. Environment
20
WATER
 Composed of H and O in the ratio
of 2:1 (H2O)
 Largest single constituent of the
body mass of animals and may
differ due to age and nutritional
status
 90% of embryos
 70% of new-born animals
 50% to 65% of body mass of
adult
 Water is the most essential dietary
nutrient (indispensable)
 Excessive loss of H2O is fatal
 10% causes serious disorders
 20% results in death
21
SOURCES OF WATER
(a) Drinking water: Major source of H2O and consumed from outside sources
 Domestic farm animals all require copious amounts of water when producing at a high level
particularly when they are heat-stressed
(b) Water contained in feed: Moisture content of feed supply water to animals
 H2O content of feedstuffs consumed by animals is highly variable
 E.g., commercial diets for poultry contain approximately 10% moisture
 In forage, may range from 5 – 7% (mature plants and hays) to ≥90% (lush young vegetation)
(c) Metabolic Water (water of oxidation): Obtained from oxidation of organic components or
metabolism of nutrients in body cells
 Catabolism of I kg of fat, carbohydrate or protein produces 1190g, 560g or 450g of water
22
FUNCTIONS OF WATER
 Absorption and transportation of nutrients to other parts of the body
 Temperature regulation
 Growth, digestion, reproduction, lactation, and lubrication of joints
 Gives body shape or form to cells
 Essential part of foodstuff. Makes food soft and palatable
 Involved in most biochemical & physiological reactions
 Essential constituents of almost all the juices or secretions of body
 Excretion of waste products from the body in the form of urine, faeces
 Helps in homeostasis (acid-base balance, pH, osmotic pressure, electrolyte
concentration)
23
LOSS OF WATER
 Urine:
 Acts as solvent for products excreted from the kidney
 Increases when excess water is consumed or diuretics (alcohol & caffeine)
 Faeces:
 May exceed urinary water in ruminants such as cattle
 Consumption of fibrous feeds results in excretion of higher amount of fecal water
 Evaporation from Body Surface and Respiratory Tracts:
 Water loss through vaporization from the lungs and dissipation through the skin and
sweat from the sweat glands in the skin during warm or hot weather
 Accounts for relatively large amount of total water loss
24
WATER REQUIREMENTS
Difficult to state/predict and depends on several factors
 Environmental Factors:
 ↑ Env’tal temp and humidity ↑ H2O requirement
 Accessibility: greater the distance between water and feed the less water the animal will
consume
 Consumption of fibrous feeds results in excretion of higher amount of fecal water
 Dietary Factors:
 DM intake is highly correlated with water intake at moderate temp
 Water content of feed affects total water intake
 Protein level of diet: High levels of protein increases water intake
 Increases in fat and salt intake increase water requirement
25
WATER REQUIREMENTS CONT’D
 Animal Factors:
 Age, stage of growth, level of production, activity, health condition and pregnancy has
direct effect on water requirement
 Frequency and periodicity of watering
 Social or behavioural interaction of animals with the environment
 Water Metabolism:
 Includes absorption, homeostasis and excretion
 Absorption: occurs in all parts of the GIT mainly large intestine
 Homeostasis: maintenance of uniformity and stability of water, balance affected by water intake
and water losses
 Excretion: H2O is excreted from body by evaporation through skin, perspiration through expired
air, and through faeces, urine, milk, tear, and saliva
26
WATER DEPRIVATION/ RESTRICTION
 Moderate Restriction:
 Reduced feed intake and
productivity
 Reduced urine and fecal water
excretion
 Severe Restriction:
 Rapid weight loss
 Dehydration accompanied by
increased renal excretion of N and
electrolytes
 Increased respiration rate
 Increased blood concentration
 Decreased blood pressure and
cardiac output
 Discomfort and incoordination in
movement
 Nausea
 Prostration and eventually death if
deprivation continues
27
ENERGY
 Energy is not a nutrient but x’tics
of some feeds
 Energy is stored in Carbohydrate,
Fats and Proteins
 Functions
 Growth
 Production (milk, meat, eggs)
 Maintenance of normal body temp
 Carrying out of vital physical
activities
 Symptoms of Energy Deficiency
 Increased feed intake
 Weight loss
 Wasting of tissues
 Death in extreme cases
28
CARBOHYDRATES (CHO)
 Defined as polyhydroxy aldehyde, ketones or acids and their derivatives or
compounds that yield these derivatives on hydrolysis
 Neutral chemical compounds containing the element C, H and O with the
empirical formula (CH2O)n, where n ≥ 3 e.g. Glucose (C6H12O6)
 Basic units of CHO is Monosaccharides
 CHO serves as both structural and reserve material in the plant
 The animal body contains < 1% CHO, which are present in blood, muscles and
liver
 The carbohydrate present in animal body is also known as glycogen
29
CARBOHYDRATES CONT’D
Based on digestibility and solubility, CHO can be divided into two groups
(a) Soluble Carbohydrates: Called nitrogen free extract (NFE) and
include simple sugar and starch, which are easily digestible in the
body
(b) Insoluble Carbohydrates: Include hard fibrous substance like crude
fibre, cellulose, hemicellulose and lignin
 Less digestible by non-ruminants and easily digested in ruminants by
rumen microflora and microfauna
30
FUNCTIONS OF CARBOHYDRATES
1. Serves as a major source of energy in animal body
2. Involved in temp production, temp control and proper functioning of
different parts of the animal body
3. Essential components of milk as lactose
4. Stored as glycogen, excess of CHO in the diet is converted into fat and
stored in the fat depot. These are reserve energy materials of the body
in liver and muscles of animals and starch in plants
5. Aids in the absorption of Ca and P in younger animals
31
FUNCTIONS OF CARBOHYDRATES
6. Help in the secretion of digestive juices in GIT
7. Provide suitable environment for the growth of rumen bacteria and
protozoa
8. Help in peristaltic movement of food
9. Maintain the glucose level of plasma
10. Component of several important bio-chemical compounds such as
nucleic acids, coenzymes and blood group substance
11. Play a key role in the metabolism of amino acids and fatty acids
32
CLASSIFICATION OF CARBOHYDRATES
CHO is divided into 2 major groups:
1. SUGARS: Generally restricted to those
carbohydrates, which contain < 10
monosaccharide residues
 Subdivided into 2 groups: Mono & Oligo
a) Monosaccharides: Simplest sugars and
cannot be hydrolyzed into smaller units.
Further divided based on the number of C
atoms. E.g. Triose (C3H603), Tetroses
(C4H604), Pentoses (C5H10O5) and Hexoses
(C6H1206)
33
CLASSIFICATION OF CARBOHYDRATES
b) Oligosaccharides: Includes all
sugars except monosaccharides
 Monosaccharides linked together with
elimination of H2O at each linkage and
 Produces di, tri, tetra or polysaccharide
containing 2,3,4 and large number of
simple sugar molecules, respectively
34
CLASSIFICATION OF CARBOHYDRATES
2. NON-SUGARS: Tasteless, insoluble, amorphous compounds with a
high molecular weight
Divided into 2 sub-groups: Polysaccharides and Complex CHO
I. Polysaccharides: Polymers of monosaccharide units. Grouped into
a) Homopolysaccharides: Single type of monosaccharide unit upon hydrolysis
 Classified according to the kind of sugar which produces the hydrolysis
 E.g. Glucans are condensation polymer of glucose, fructans of fructose and
xylans of xylose
35
CLASSIFICATION OF CARBOHYDRATES
b) Heteropolysaccharides: Mixed polysaccharides, which on
hydrolysis yield mixtures of monosaccharides and derived
products
II. Complex CHO: ill-defined group of compounds that contain CHO in
combination with non-carbohydrate molecules. They include the
glycolipids and glycoproteins
36
CLASSIFICATION OF CARBOHYDRATES
37
LIPIDS
 Lipids are a group of substances found in plants and animals, which are
insoluble in water but soluble in organic solvents such as ether, chloroform
and benzene
 They act as electron carriers, as substrate carriers in enzymic reactions, as
components of biological membranes, and as sources and stores of energy
 Produces 2.25 times the energy of CHO or proteins
 Most are triglyceride (glycerol + 3 fatty acids)
 Fats are lipids solid at room temp whilst Oils are liquid at room temp
38
FUNCTIONS OF LIPIDS
1. Main function of fats is to supply energy to the animal body.
One gram of fat after complete oxidation produces 9.3 Kcal
heat. Fats are reserved source of energy to the animal body
2. After hydrolysis, fats are converted into fatty acid and glycerol,
thus providing essential fatty acids (linoleic, arachidonic and
linolenic) to the body
3. Essential component of milk
4. Helps in the absorption of Ca and P in the body
39
FUNCTIONS OF LIPIDS
5. Aid in the absorption of fat soluble vitamins (A,D,E, and K) in the
blood
6. Essential constituent of the body protoplasm.
7. Helps in temp regulation & insulation for vital organs, protecting them
from shock.
8. Required for the lubrication of joints
9. Fats are important nutrient of nervous metabolism
10. Delays the sensation' of hunger, as it requires a longer period of time
to pass through the stomach than carbohydrate and protein
40
CLASSIFICATION OF LIPIDS
 Categorized into 3 main
groups
1. Simple Lipids:
 Are esters of fatty acids with
various alcohols, particularly
glycerol and cholesterol
 Fats, oils, and waxes are simple
lipids
 Fats and oil are esters of fatty
acids with glycerol and waxes are
esters of fatty acids with an
alcohol
2. Compound lipids:
 Are esters of glycerol which contain two
fatty acid residues plus another
chemical group such as choline (linked
through phosphoric acid)
 They include phospholipids,
glycolipids, and lipoproteins
41
CLASSIFICATION OF LIPIDS
2. Compound lipids:
 They include phospholipids, glycolipids, and lipoproteins
 Phospholipids are fats containing phosphoric acid and N
 Glycolipids are fats containing CHO and often N
 Lipoproteins are lipids bound to proteins in blood and other tissues
3. Derived lipids:
 Substances derived from groups simple and compound lipids by
hydrolysis, - i.e., fatty acids, glycerol, and other alcohols such as
cholesterol, ergosterol and sitosterol
42
CLASSIFICATION OF LIPIDS
43
STRUCTURE OF LIPIDS
44
 Fats and Oils have the same general
structure and chemical properties but
different physical characteristics
 The melting point of the oils is such that
at ordinary room temperature they are
liquid while fats are solid
 Chemically fats are ester of fatty acid
with glycerol
 In nature 3 fatty acid molecules combine
with one glycerol molecule with release
of three molecules of water
FATTY ACIDS (FA)
45
 FA are the building blocks of fat
 Consist of chains of C atoms ranging from 2 to 30 or more C’s in length
with a carboxyl group on the end of each chain
 The general structure is RCOOH, where R is a chain of variable length
 The chain may be saturated (containing only single bonds) or unsaturated
(containing one or more double bonds)
FATTY ACIDS (S FA)
46
Common Name No. of C Atoms Systemic Name
Acetic 2 Ethanoic
Propionic 3 Propionic
Butyric 4 Butanoic
Caproic 6 Hexanoic
Caprylic 8 Octanoic
Capric 10 Decanoic
Lauric 12 Dodecanoic
Myristic 14 Tetra decanoic
Palmitic 16 Hexa decanoic
Stearic 18 Octa decanoic
FATTY ACIDS
47
Types of Fatty Acids
 Essential FA: They FA that cannot be synthesized by the body of
animals or in sufficient amount to carry out physiological activities
Linoleic, Linolenic, and Arachidonic acids
 Non-essential FA: Those that can be synthesized by the body and
thus not a dietary requirement
 Most fatty acids except the 3
FATTY ACIDS (U FA)
48
GLYCEROL
49
 The alcohol component of all tri-glycerides common in animal and
plant tissues and is a component of lecithin, cephalin and
sphingomyelin
Mono-, di-, and triglycerides
 These are esters of glycerol and fatty acids: - The fatty acid
composition of triglycerides is variable.
 The chain length and degree of unsaturation of the individual fatty
acids making up the triglyceride determines its physical and chemical
properties
GLYCEROL
50
 For example, simple triglycerides of saturated fatty acids
containing 10 or more Cs are solid at room temperature,
whiles those with less than 10 Cs usually are liquid.
 Triglycerides containing only long-chain saturated fatty acids
are solids, whereas those containing more of unsaturated fatty
acids are liquids.
PROTEINS (CHON)
 Proteins are complex organic nitrogenous compounds made up of
amino acids (basic units)
 All proteins contain C, H, O, N and generally S, many contain P
 Element such as I, Fe, Cu and Zn are also occasionally present
 Each species has its own specific proteins, and a single
organism has many different proteins in its cells and tissues
51
PROTEINS (CHON)
 Most proteins contain about 16% N, which means that the weight of
protein N multiplied by 6.25 (100/16 = 6.25) equal the weight of protein
 E.g. Suppose a feed sample to be analyzed yields 1.0 gram of N by
Kjeldahl process, then the weight of protein is calculated as 1.0 x 6.25 =
6.25 g
 Milk N is multiplied by 6.38 because milk protein contains 15.87 percent
N
52
AMINO ACIDS (AA)
 AAs are produced when proteins are hydrolyzed by enzymes, acids or
alkalis
 Although over 200 AA have been isolated from biological materials,
only 20 are commonly found as components of proteins
Structure of Amino Acids
 Amino acids have a basic amino group (–NH2) and an acidic carboxyl
group (–COOH)
 AA are amphoteric (both acidic and basic properties) in nature and
exist as dipolar ions in aqueous solution
53
AMINO ACIDS (AA)
 Most AAs occurring naturally in proteins are of the α type,
having the amino group attached to the C atom adjacent to
the carboxyl group
54
CLASSIFICATION OF AMINO ACIDS
 AAs can be classified into 3 groups, namely, the aliphatic, aromatic and
heterocyclic amino acids
1. Aliphatic: AA containing an aliphatic side chain functional group. They
are nonpolar and hydrophobic. Hydrophobicity ↑ with ↑sing # of C
atoms
Alanine, Glycine (simplest AA), Isoleucine, Leucine, Serine,
Threonine, Valine, Aspartic Acid, Lysine, Glutamic Acid
Cystine, Methionine, Cysteine (S containing AA)
55
CLASSIFICATION OF AMINO ACIDS
2. Aromatic: AA with little or no charge. Such AA have stable aromatic
ring that does not easily react with other compounds or elements
Phenylalanine, and Tyrosine,
3. Heterocyclic: AA forming a ring made up of C atoms and at least one
atom other than C
Histidine, Proline, Hydroxyproline, Tryptophan
56
ESSENTIAL AND NON-ESSENTIAL AMINO
ACIDS
1. Essential AA: AA that can not be synthesized by the
body and must be included in the diet
(Phenylalanine, Valine, Threonine), (Tryptophan, Isoleucine,
Methionine), (Histidine, Arginine, Leucine, Lysine)
PVT TIM HALL
57
ESSENTIAL AND NON-ESSENTIAL AMINO
ACIDS
2. Nonessential AA: AA that can be synthesized in the body,
hence not a dietary requirement
 Alanine, Aspartic acid, Cystine, Glutamic acid, Glycine,
Hydroxyproline, Proline, Serine, Tyrosine, & Glutamine
 A limiting amino acid is one present in the lowest amount
relative to the requirement
Lysine (Swine), methionine (Poultry) & tryptophane are likely to be
limiting in diets for monogastric animals
58
ESSENTIAL AMINO ACIDS
 Phenylalanine
 Valine
 Threonine
 Methionine
 Arginine
 Tryptophane
 Histidine
 Isoleucine
 Leucine
 Lysine
PVT MAT HILL
PVT TIM HALL
59
NONESSENTIAL AMINO ACIDS
 Alanine
 Aspartic Acid
 Citrulline
 Cystine
 Glutamic Acid*
* Required by chick for optimal
growth
 Glycine*
 Hydroxyproline
 Proline*
 Serine
 Tyrosine
60
CLASSIFICATION OF PROTEINS
 Proteins are classified based on their
shape, chemical composition, solubilities
in water, salt, acids, bases and alcohol
1. Globular Proteins: Soluble in water or in
dilute acids or bases or in alcohol. Includes
all the enzymes, antigens and hormones
 Albumins, Globulins, Glutelins, Prolamines,
Histones, Protamines
2. Fibrous Proteins: Insoluble in water and
resistant to digestive enzymes of animals
 Collagens – the major protein of skeletal
connective tissues
 Elastins – the proteins of the elastic tissues such
as tendons and arteries
 Keratins – proteins of feathers, hairs, claws,
beak, hoofs and horns
3. Conjugated Proteins: composed of simple
protein combined with some non-protein
substances as prosthetic group
 Nucleoproteins, Muccoids, Glycoproteins,
Lipoproteins, Chymoproteins, Metalloproteins
61
FUNCTIONS OF PROTEINS
1. Proteins form muscles and tissues of the body;
hence it is essential for the growth and
development of the body.
2. Help in maintaining the loss of body tissues and
muscles.
3. Help in the formation of enzymes, hormones,
antigen, antibody, digestive juices of the body
and regulate body osmotic pressure and acid-
base balance.
4. Help in the repair of body cells as well as for the
production of new cells.
5. Also supply energy to the body in the absence
of CHO and Lipids
6. Essential for the formation of egg, milk protein,
wool and hairs of the animals.
7. Provide the basic cellular matrix within which the
bone mineral matter is deposited.
8. Under condition of non-digestion and no-
chances for denaturation, the protein
accumulates inside the cells and produce toxicity.
i.e. venoms of snakes and insects are infected by
biting into the blood.
9. Endorphins (peptide) are found in brain and are
involved in the suppression of pain.
62
SIGNS OF PROTEIN DEFICIENCY
1. Anorexia
2. Reduced growth rate
3. Reduced or negative N balance
4. Reduced efficiency of feed utilisation
5. Reduced serum protein concentration
6. Anaemia
7. Fat accumulation in the liver
8. Oedema (in severe case)
9. Reduced birth weight of young
10. Reduced milk production
11. Reduced egg production in poultry
12. Reduced synthesis of certain enzymes
and hormones
13. In severe protein or amino acid
deficiency, growth is arrested
completely
63
Signs of Excessive Intake of Protein
1. Above certain protein intake, there
is a linear depression in weight gain
with increasing protein.
2. Feed intake decreases
3. Hair becomes dull and coarse
4. High protein diet reduces activity of
several adipose tissue enzymes
associated with fatty acid synthesis
in pigs.
5. Ammonia toxicity is a practical
problem in ruminants fed urea as a
NPN source. Toxic symptoms in
ruminants include: uneasiness,
laboured breathing, excessive
salivation, muscle and skin tremors,
incoordination, tetany and death
within 2h of onset of symptoms.
64
MINERALS
 Composed of single solid substance of
uniform composition that cannot be
physically separated into simpler chemical
compounds
 About 40 mineral elements occur in
measurable amount in nature in plants and
animals tissues
 At least 21 of these have been shown to be
required by animals
 Minerals are generally classified into two
categories
1. Macro (Major) Elements : Minerals required
in relatively large amount by the body for
normal growth and development
 Mostly used in the synthesis of structural
tissues
2. Micro (Minor or Trace) Elements:
Minerals required in relatively small
amount for the physiological processes
of the body
 Usually function as activators or as a
component of enzyme system
65
MINERALS
MACRO
 Calcium
 Phosphorous
 Sodium
 Chlorine
 Sulphur
 Potassium
 Magnesium
MICRO
 Iron & Copper
 Iodine & Fluorine
 Cobalt
 Manganese
 Molybdenum
 Selenium
 Zinc
66
GENERAL FUNCTION OF MINERALS
 As a constituent of skeletal structure
 In regulating acid-base equilibrium
 They are helpful in maintaining the colloidal state of body matter and
regulating some of the physical properties of colloidal systems like
viscosity, diffusion and osmotic pressure
 They act as a component or an activator of enzymes and or other
biological systems
67
FUNCTIONS OF SOME MINERALS
68
VITAMINS
 Vitamins are the substances distinct from major components of food required in
minute quantities and whose absence causes specific deficiency disease
 Organic substances required by animals in very small amounts for regulating
various body processes toward normal health, growth, production and reproduction
 Cannot be synthesised by the animal and therefore must be obtained from the diet
 Exception: vitamin D may be synthesised on the surface of the skin by ultra-violet
irradiation, and nicotinic acid is synthesised to some extent from tryptophan
69
CLASSIFICATION OF VITAMINS
 Vitamins are grouped into 2 based upon
their solubilities in either fats and fat
solvents or in water
1. Fat Soluble vitamins: Vitamins that
dissolve in fat
 Found in in feedstuffs in association with
the lipids.
 These vitamins are absorbed along with
the dietary fats by mechanisms similar to
those involved in fat absorption
 They are stored in appreciable quantities in
the animal body and are not excreted in
the urine
2. Water-Soluble: Vitamins that readily
dissolves in water
 Except for B12, are not stored but excesses
are rapidly excreted in the urine
 A constant dietary supply of the water-
soluble vitamins is needed to avoid
deficiencies
70
FAT-SOLUBLE VITAMINS
 Fat-soluble vitamins include Vit A, D, E, K
1. A - maintenance of epithelial tissue
2. D - calcium & phosphorous metabolism
3. E - antioxidant – (selenium)
4. K - blood clotting
71
WATER SOLUBLE VITAMINS
 B1- Thiamine
 B2- Riboflavin
 B6- Pyridoxine
 Pantothenic Acid
 Folic Acid
 Niacin
 B12- Cyanocobalamin
 Choline
 Biotin
 C - Ascorbic Acid (not in
livestock)
72
IMPORTANT VITAMINS IN ANIMAL
NUTRITION
73
GENERAL FUNCTIONS OF VITAMINS
1. Vitamins are essential for the good health and play important role in the
body growth
2. Provide resistance against diseases and increases the productivity power of
animals.
3. Essential constituents of certain enzyme systems, regulate body metabolism
and clotting of blood.
4. Required during pregnancy for the development of foetus.
5. Vitamin ‘A' is responsible for the proper functioning of vision and Vitamin C
keeps the gums in healthy state
74
FUNCTIONS OF SOME VITAMINS
75

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  • 1. AS 252 PRINCIPLES OF ANIMAL NUTRITION BY FRANK IDAN, PH.D. LECTURER DEPARTMENT OF ANIMAL SCIENCE, KNUST EMAIL: frank.idan@knust.edu.gh/frankkidan@gmail.com MOB: 0243389831
  • 2. BACKGROUND 2  Teaching Philosophy Every student has the potential to excel when given the right opportunities and guidance Students need to stay focus, work hard, and be committed to their academic work
  • 3. LECTURE OUTLINE 3  What is Nutrition and Nutrient?  Nutritional Requirements  Components of Plants and Animals  Six Classes of Nutrients and Functions of Nutrients Water, CHO, Protein, Lipids, Vitamins and Minerals
  • 4. THE SCIENCE OF NUTRITION  What is Nutrition?  The science of nutrition examines the qualitative and quantitative requirements of the diet necessary to maintain proper health of animals  Involves various chemical reactions and physiological processes which transform food into body tissues and activities  Qualitative Requirements: Essential nutrients  Quantitative Requirements: Balanced diet – correct nutrients, in proper amounts, that is required by animals to stay healthy 4
  • 5. DEFINITIONS AND TERMS  Nutrients: Any chemical element or compound in the diet that is required for normal life processes  Components of food capable of being utilised by animals  Presently, more than 100 nutrients are needed in animal diets  Food: An edible material that provides nutrients  Material which, after ingestion by animals, is capable of being digested, absorbed, and utilised  Animals categorized into Herbivores, Carnivores and Omnivores depending on the type of food  Feed: commonly used to designate animal food 5
  • 6. DEFINITIONS AND TERMS  Diets: A mixture of feedstuffs used to supply nutrients to an animal  Ration: An amount of feed allocated to an animal in a day i.e. how much feed an animal gets per day  Feed: commonly used to designate animal food  Foodstuff/Feedstuff: Any material made into or used as food for animals 6
  • 7. NUTRITIONAL REQUIREMENTS  Plants vs Animals: Both plants and animals require Water and Energy  Autotrophic: Type of nutrition in which organisms synthesize their own food from simple inorganic materials such as H2O, CO2 and mineral salts in the presence of sunlight  Plants and other chlorophyll containing organisms  Process called Photosynthesis which is the opposite of respiration in Animals  6 CO2 + 6H2O C6H12O6 + 6 O2  Heterotrophic: Mode of nutrition in which organisms depend on other organisms for their food to survive  Cannot manufacture own food  Mostly animals, fungi, and other non-photosynthetic organisms 7 6CO2 + 6H2O C6H12O6 Sunlight Chlorophyll
  • 8. NUTRITIONAL REQUIREMENTS  Essential Nutrients for Most Animals:  Water  Energy* = Carbohydrates, Lipids and Proteins  Amino Acids (Nitrogen sources)  Fatty Acids  Minerals = Macro and Micro  Vitamins = Fat and Water soluble  Other Nutrients (Species dependent) – Taurine, Choline, Carnitine etc. 8 6CO2 + 6H2O C6H12O6
  • 9. FACTORS AFFECTING NUTRITIONAL REQUIREMENTS  Gastrointestinal Tracts of Species Carnivores, Herbivores, and Omnivores Energy sources Carnivores/Omnivores – Fat/Protein Herbivores - Carbohydrates  Age  Level of Productivity  Production, Gestation, and Lactation  Environmental Factors  Temperature, Humidity 9 6CO2 + 6H2O C6H12O6
  • 10. COMPOSITION OF ANIMAL FEED 10 6CO2 + 6H2O C6H12O6 What is actually contained in Animal Feed? 1. Moisture (Water) 2. Dry Matter a. Organic Material: (N-containing compounds (proteins), lipids (fat soluble), carbohydrates (water soluble), vitamins, nucleic acids, and organic acids b. Inorganic Material (Ash): Minerals Macro and micro/trace minerals; Any mineral can be toxic in excessive amounts
  • 11. COMPOSITION OF ANIMAL FEED 11 6CO2 + 6H2O C6H12O6 Plants and animals contain similar types of chemical substances, can be grouped into classes according to constitution, properties and function
  • 12. COMPOSITION OF PLANTS AND ANIMALS  Plants – Mostly water and carbohydrates CHO- Readily available (sugars) vs fibrous components (crude fibre) Low in protein and fat  Animals – Mostly fat and protein 60% water, 20% fat, 16% protein, and 4% minerals Very low in carbohydrates (< 1%) 12
  • 13. THE CHEMICAL COMPOSITION OF PLANTS AND ANIMALS BODY  Physical and chemical processes occurring in the protoplasm represents the chemical composition of plant and animal  Animal body derives all nutrients for its physiological functions from the digestion of plant and plant products as well as animal products such as fish meal and milk  Knowledge of the chemical composition of farm animals is required to understand their nutrient requirement while that of plants is essential because they furnish most of the food for livestock 13
  • 14. THE CHEMICAL COMPOSITION OF PLANTS AND ANIMALS BODY CONT’D  Plants and animals' tissues are composed of similar type of chemical substances, but their relative amounts vary  Plants are analyzed by proximate method of analysis whereas, animal body was first analyzed by Lawes and Gilbert (1858)by slaughter experiments  Plants and their by-products show much larger differences in the chemical composition than the animals  Proportions of various body organs of animals change during the period before and immediately after birth and during post-natal growth 14
  • 15. COMPARISON OF PLANTS AND ANIMALS BODY COMPOSITION Parameters Animal Plant Major constituent Water Water Major Organic Constituent Protein Carbohydrate (CHO) Structural Component Protein and Mineral CHO (Cellulose, Lignin Hemicellulose etc.) Reserve Material Fat (Glycogen) CHO (Starch) CHO Amount Less More Minerals Amount Generally constant to species Wide variation Variations in Composition Less Wide 15
  • 16. Table 1: Chemical Composition of The Pig’s Body During Early Post-natal Life 16 Post Natal Period (days) 0 7 14 28 Empty BW (g) 1450 3044 5248 9651 Lipid (%) 1.2 10.1 15.1 18.3 Protein (%) 12.0 14.4 14.4 14.6 Ash (%) 4.2 3.1 3.0 3.3 Water (%) 82.6 72.5 67.3 63.6
  • 17. SIX CLASSES OF NUTRIENTS  Water  Carbohydrates (CHO)  Lipids (Fats & Oils)  Proteins (CHON)  Minerals  Vitamins 17
  • 18. FUNCTIONS OF NUTRIENTS 1. Source of Energy: CHO, lipids and proteins  Energy needed for heat production, fat deposition, and work 2. Maintenance: All nutrients  Nutrients are needed for breathing, movement, digestion, maintaining body weight, keeping warm etc. 3. Growth: Proteins  Most rapid at early stages of life  Increase in height, weight, building of tissues 4. Reproduction: All nutrients  Development of maternal tissues and foetus 18
  • 19. FUNCTIONS OF NUTRIENTS CONT’D 5. Structural: Proteins, minerals, fats, and water  Maintenance of structural Integrity  Building/maintaining body structure, cushioning of body organs e.g., Heart 6. Regulatory: Vitamins, minerals, protein, and water  Transport of nutrients, stimulating or catalyzing activities of hormones or enzymes needed for particular metabolic processes 7. Production: All essential nutrients  Meat, Milk, and Egg production 19
  • 20. FACTORS AFFECTING NUTRIENT REQUIREMENTS 1. Species 2. Breeds 3. Weight 4. Body condition 5. Growth 6. Stage of gestation 7. Level of milk production 8. Age 9. Sex 10. Work 11. Environment 20
  • 21. WATER  Composed of H and O in the ratio of 2:1 (H2O)  Largest single constituent of the body mass of animals and may differ due to age and nutritional status  90% of embryos  70% of new-born animals  50% to 65% of body mass of adult  Water is the most essential dietary nutrient (indispensable)  Excessive loss of H2O is fatal  10% causes serious disorders  20% results in death 21
  • 22. SOURCES OF WATER (a) Drinking water: Major source of H2O and consumed from outside sources  Domestic farm animals all require copious amounts of water when producing at a high level particularly when they are heat-stressed (b) Water contained in feed: Moisture content of feed supply water to animals  H2O content of feedstuffs consumed by animals is highly variable  E.g., commercial diets for poultry contain approximately 10% moisture  In forage, may range from 5 – 7% (mature plants and hays) to ≥90% (lush young vegetation) (c) Metabolic Water (water of oxidation): Obtained from oxidation of organic components or metabolism of nutrients in body cells  Catabolism of I kg of fat, carbohydrate or protein produces 1190g, 560g or 450g of water 22
  • 23. FUNCTIONS OF WATER  Absorption and transportation of nutrients to other parts of the body  Temperature regulation  Growth, digestion, reproduction, lactation, and lubrication of joints  Gives body shape or form to cells  Essential part of foodstuff. Makes food soft and palatable  Involved in most biochemical & physiological reactions  Essential constituents of almost all the juices or secretions of body  Excretion of waste products from the body in the form of urine, faeces  Helps in homeostasis (acid-base balance, pH, osmotic pressure, electrolyte concentration) 23
  • 24. LOSS OF WATER  Urine:  Acts as solvent for products excreted from the kidney  Increases when excess water is consumed or diuretics (alcohol & caffeine)  Faeces:  May exceed urinary water in ruminants such as cattle  Consumption of fibrous feeds results in excretion of higher amount of fecal water  Evaporation from Body Surface and Respiratory Tracts:  Water loss through vaporization from the lungs and dissipation through the skin and sweat from the sweat glands in the skin during warm or hot weather  Accounts for relatively large amount of total water loss 24
  • 25. WATER REQUIREMENTS Difficult to state/predict and depends on several factors  Environmental Factors:  ↑ Env’tal temp and humidity ↑ H2O requirement  Accessibility: greater the distance between water and feed the less water the animal will consume  Consumption of fibrous feeds results in excretion of higher amount of fecal water  Dietary Factors:  DM intake is highly correlated with water intake at moderate temp  Water content of feed affects total water intake  Protein level of diet: High levels of protein increases water intake  Increases in fat and salt intake increase water requirement 25
  • 26. WATER REQUIREMENTS CONT’D  Animal Factors:  Age, stage of growth, level of production, activity, health condition and pregnancy has direct effect on water requirement  Frequency and periodicity of watering  Social or behavioural interaction of animals with the environment  Water Metabolism:  Includes absorption, homeostasis and excretion  Absorption: occurs in all parts of the GIT mainly large intestine  Homeostasis: maintenance of uniformity and stability of water, balance affected by water intake and water losses  Excretion: H2O is excreted from body by evaporation through skin, perspiration through expired air, and through faeces, urine, milk, tear, and saliva 26
  • 27. WATER DEPRIVATION/ RESTRICTION  Moderate Restriction:  Reduced feed intake and productivity  Reduced urine and fecal water excretion  Severe Restriction:  Rapid weight loss  Dehydration accompanied by increased renal excretion of N and electrolytes  Increased respiration rate  Increased blood concentration  Decreased blood pressure and cardiac output  Discomfort and incoordination in movement  Nausea  Prostration and eventually death if deprivation continues 27
  • 28. ENERGY  Energy is not a nutrient but x’tics of some feeds  Energy is stored in Carbohydrate, Fats and Proteins  Functions  Growth  Production (milk, meat, eggs)  Maintenance of normal body temp  Carrying out of vital physical activities  Symptoms of Energy Deficiency  Increased feed intake  Weight loss  Wasting of tissues  Death in extreme cases 28
  • 29. CARBOHYDRATES (CHO)  Defined as polyhydroxy aldehyde, ketones or acids and their derivatives or compounds that yield these derivatives on hydrolysis  Neutral chemical compounds containing the element C, H and O with the empirical formula (CH2O)n, where n ≥ 3 e.g. Glucose (C6H12O6)  Basic units of CHO is Monosaccharides  CHO serves as both structural and reserve material in the plant  The animal body contains < 1% CHO, which are present in blood, muscles and liver  The carbohydrate present in animal body is also known as glycogen 29
  • 30. CARBOHYDRATES CONT’D Based on digestibility and solubility, CHO can be divided into two groups (a) Soluble Carbohydrates: Called nitrogen free extract (NFE) and include simple sugar and starch, which are easily digestible in the body (b) Insoluble Carbohydrates: Include hard fibrous substance like crude fibre, cellulose, hemicellulose and lignin  Less digestible by non-ruminants and easily digested in ruminants by rumen microflora and microfauna 30
  • 31. FUNCTIONS OF CARBOHYDRATES 1. Serves as a major source of energy in animal body 2. Involved in temp production, temp control and proper functioning of different parts of the animal body 3. Essential components of milk as lactose 4. Stored as glycogen, excess of CHO in the diet is converted into fat and stored in the fat depot. These are reserve energy materials of the body in liver and muscles of animals and starch in plants 5. Aids in the absorption of Ca and P in younger animals 31
  • 32. FUNCTIONS OF CARBOHYDRATES 6. Help in the secretion of digestive juices in GIT 7. Provide suitable environment for the growth of rumen bacteria and protozoa 8. Help in peristaltic movement of food 9. Maintain the glucose level of plasma 10. Component of several important bio-chemical compounds such as nucleic acids, coenzymes and blood group substance 11. Play a key role in the metabolism of amino acids and fatty acids 32
  • 33. CLASSIFICATION OF CARBOHYDRATES CHO is divided into 2 major groups: 1. SUGARS: Generally restricted to those carbohydrates, which contain < 10 monosaccharide residues  Subdivided into 2 groups: Mono & Oligo a) Monosaccharides: Simplest sugars and cannot be hydrolyzed into smaller units. Further divided based on the number of C atoms. E.g. Triose (C3H603), Tetroses (C4H604), Pentoses (C5H10O5) and Hexoses (C6H1206) 33
  • 34. CLASSIFICATION OF CARBOHYDRATES b) Oligosaccharides: Includes all sugars except monosaccharides  Monosaccharides linked together with elimination of H2O at each linkage and  Produces di, tri, tetra or polysaccharide containing 2,3,4 and large number of simple sugar molecules, respectively 34
  • 35. CLASSIFICATION OF CARBOHYDRATES 2. NON-SUGARS: Tasteless, insoluble, amorphous compounds with a high molecular weight Divided into 2 sub-groups: Polysaccharides and Complex CHO I. Polysaccharides: Polymers of monosaccharide units. Grouped into a) Homopolysaccharides: Single type of monosaccharide unit upon hydrolysis  Classified according to the kind of sugar which produces the hydrolysis  E.g. Glucans are condensation polymer of glucose, fructans of fructose and xylans of xylose 35
  • 36. CLASSIFICATION OF CARBOHYDRATES b) Heteropolysaccharides: Mixed polysaccharides, which on hydrolysis yield mixtures of monosaccharides and derived products II. Complex CHO: ill-defined group of compounds that contain CHO in combination with non-carbohydrate molecules. They include the glycolipids and glycoproteins 36
  • 38. LIPIDS  Lipids are a group of substances found in plants and animals, which are insoluble in water but soluble in organic solvents such as ether, chloroform and benzene  They act as electron carriers, as substrate carriers in enzymic reactions, as components of biological membranes, and as sources and stores of energy  Produces 2.25 times the energy of CHO or proteins  Most are triglyceride (glycerol + 3 fatty acids)  Fats are lipids solid at room temp whilst Oils are liquid at room temp 38
  • 39. FUNCTIONS OF LIPIDS 1. Main function of fats is to supply energy to the animal body. One gram of fat after complete oxidation produces 9.3 Kcal heat. Fats are reserved source of energy to the animal body 2. After hydrolysis, fats are converted into fatty acid and glycerol, thus providing essential fatty acids (linoleic, arachidonic and linolenic) to the body 3. Essential component of milk 4. Helps in the absorption of Ca and P in the body 39
  • 40. FUNCTIONS OF LIPIDS 5. Aid in the absorption of fat soluble vitamins (A,D,E, and K) in the blood 6. Essential constituent of the body protoplasm. 7. Helps in temp regulation & insulation for vital organs, protecting them from shock. 8. Required for the lubrication of joints 9. Fats are important nutrient of nervous metabolism 10. Delays the sensation' of hunger, as it requires a longer period of time to pass through the stomach than carbohydrate and protein 40
  • 41. CLASSIFICATION OF LIPIDS  Categorized into 3 main groups 1. Simple Lipids:  Are esters of fatty acids with various alcohols, particularly glycerol and cholesterol  Fats, oils, and waxes are simple lipids  Fats and oil are esters of fatty acids with glycerol and waxes are esters of fatty acids with an alcohol 2. Compound lipids:  Are esters of glycerol which contain two fatty acid residues plus another chemical group such as choline (linked through phosphoric acid)  They include phospholipids, glycolipids, and lipoproteins 41
  • 42. CLASSIFICATION OF LIPIDS 2. Compound lipids:  They include phospholipids, glycolipids, and lipoproteins  Phospholipids are fats containing phosphoric acid and N  Glycolipids are fats containing CHO and often N  Lipoproteins are lipids bound to proteins in blood and other tissues 3. Derived lipids:  Substances derived from groups simple and compound lipids by hydrolysis, - i.e., fatty acids, glycerol, and other alcohols such as cholesterol, ergosterol and sitosterol 42
  • 44. STRUCTURE OF LIPIDS 44  Fats and Oils have the same general structure and chemical properties but different physical characteristics  The melting point of the oils is such that at ordinary room temperature they are liquid while fats are solid  Chemically fats are ester of fatty acid with glycerol  In nature 3 fatty acid molecules combine with one glycerol molecule with release of three molecules of water
  • 45. FATTY ACIDS (FA) 45  FA are the building blocks of fat  Consist of chains of C atoms ranging from 2 to 30 or more C’s in length with a carboxyl group on the end of each chain  The general structure is RCOOH, where R is a chain of variable length  The chain may be saturated (containing only single bonds) or unsaturated (containing one or more double bonds)
  • 46. FATTY ACIDS (S FA) 46 Common Name No. of C Atoms Systemic Name Acetic 2 Ethanoic Propionic 3 Propionic Butyric 4 Butanoic Caproic 6 Hexanoic Caprylic 8 Octanoic Capric 10 Decanoic Lauric 12 Dodecanoic Myristic 14 Tetra decanoic Palmitic 16 Hexa decanoic Stearic 18 Octa decanoic
  • 47. FATTY ACIDS 47 Types of Fatty Acids  Essential FA: They FA that cannot be synthesized by the body of animals or in sufficient amount to carry out physiological activities Linoleic, Linolenic, and Arachidonic acids  Non-essential FA: Those that can be synthesized by the body and thus not a dietary requirement  Most fatty acids except the 3
  • 48. FATTY ACIDS (U FA) 48
  • 49. GLYCEROL 49  The alcohol component of all tri-glycerides common in animal and plant tissues and is a component of lecithin, cephalin and sphingomyelin Mono-, di-, and triglycerides  These are esters of glycerol and fatty acids: - The fatty acid composition of triglycerides is variable.  The chain length and degree of unsaturation of the individual fatty acids making up the triglyceride determines its physical and chemical properties
  • 50. GLYCEROL 50  For example, simple triglycerides of saturated fatty acids containing 10 or more Cs are solid at room temperature, whiles those with less than 10 Cs usually are liquid.  Triglycerides containing only long-chain saturated fatty acids are solids, whereas those containing more of unsaturated fatty acids are liquids.
  • 51. PROTEINS (CHON)  Proteins are complex organic nitrogenous compounds made up of amino acids (basic units)  All proteins contain C, H, O, N and generally S, many contain P  Element such as I, Fe, Cu and Zn are also occasionally present  Each species has its own specific proteins, and a single organism has many different proteins in its cells and tissues 51
  • 52. PROTEINS (CHON)  Most proteins contain about 16% N, which means that the weight of protein N multiplied by 6.25 (100/16 = 6.25) equal the weight of protein  E.g. Suppose a feed sample to be analyzed yields 1.0 gram of N by Kjeldahl process, then the weight of protein is calculated as 1.0 x 6.25 = 6.25 g  Milk N is multiplied by 6.38 because milk protein contains 15.87 percent N 52
  • 53. AMINO ACIDS (AA)  AAs are produced when proteins are hydrolyzed by enzymes, acids or alkalis  Although over 200 AA have been isolated from biological materials, only 20 are commonly found as components of proteins Structure of Amino Acids  Amino acids have a basic amino group (–NH2) and an acidic carboxyl group (–COOH)  AA are amphoteric (both acidic and basic properties) in nature and exist as dipolar ions in aqueous solution 53
  • 54. AMINO ACIDS (AA)  Most AAs occurring naturally in proteins are of the α type, having the amino group attached to the C atom adjacent to the carboxyl group 54
  • 55. CLASSIFICATION OF AMINO ACIDS  AAs can be classified into 3 groups, namely, the aliphatic, aromatic and heterocyclic amino acids 1. Aliphatic: AA containing an aliphatic side chain functional group. They are nonpolar and hydrophobic. Hydrophobicity ↑ with ↑sing # of C atoms Alanine, Glycine (simplest AA), Isoleucine, Leucine, Serine, Threonine, Valine, Aspartic Acid, Lysine, Glutamic Acid Cystine, Methionine, Cysteine (S containing AA) 55
  • 56. CLASSIFICATION OF AMINO ACIDS 2. Aromatic: AA with little or no charge. Such AA have stable aromatic ring that does not easily react with other compounds or elements Phenylalanine, and Tyrosine, 3. Heterocyclic: AA forming a ring made up of C atoms and at least one atom other than C Histidine, Proline, Hydroxyproline, Tryptophan 56
  • 57. ESSENTIAL AND NON-ESSENTIAL AMINO ACIDS 1. Essential AA: AA that can not be synthesized by the body and must be included in the diet (Phenylalanine, Valine, Threonine), (Tryptophan, Isoleucine, Methionine), (Histidine, Arginine, Leucine, Lysine) PVT TIM HALL 57
  • 58. ESSENTIAL AND NON-ESSENTIAL AMINO ACIDS 2. Nonessential AA: AA that can be synthesized in the body, hence not a dietary requirement  Alanine, Aspartic acid, Cystine, Glutamic acid, Glycine, Hydroxyproline, Proline, Serine, Tyrosine, & Glutamine  A limiting amino acid is one present in the lowest amount relative to the requirement Lysine (Swine), methionine (Poultry) & tryptophane are likely to be limiting in diets for monogastric animals 58
  • 59. ESSENTIAL AMINO ACIDS  Phenylalanine  Valine  Threonine  Methionine  Arginine  Tryptophane  Histidine  Isoleucine  Leucine  Lysine PVT MAT HILL PVT TIM HALL 59
  • 60. NONESSENTIAL AMINO ACIDS  Alanine  Aspartic Acid  Citrulline  Cystine  Glutamic Acid* * Required by chick for optimal growth  Glycine*  Hydroxyproline  Proline*  Serine  Tyrosine 60
  • 61. CLASSIFICATION OF PROTEINS  Proteins are classified based on their shape, chemical composition, solubilities in water, salt, acids, bases and alcohol 1. Globular Proteins: Soluble in water or in dilute acids or bases or in alcohol. Includes all the enzymes, antigens and hormones  Albumins, Globulins, Glutelins, Prolamines, Histones, Protamines 2. Fibrous Proteins: Insoluble in water and resistant to digestive enzymes of animals  Collagens – the major protein of skeletal connective tissues  Elastins – the proteins of the elastic tissues such as tendons and arteries  Keratins – proteins of feathers, hairs, claws, beak, hoofs and horns 3. Conjugated Proteins: composed of simple protein combined with some non-protein substances as prosthetic group  Nucleoproteins, Muccoids, Glycoproteins, Lipoproteins, Chymoproteins, Metalloproteins 61
  • 62. FUNCTIONS OF PROTEINS 1. Proteins form muscles and tissues of the body; hence it is essential for the growth and development of the body. 2. Help in maintaining the loss of body tissues and muscles. 3. Help in the formation of enzymes, hormones, antigen, antibody, digestive juices of the body and regulate body osmotic pressure and acid- base balance. 4. Help in the repair of body cells as well as for the production of new cells. 5. Also supply energy to the body in the absence of CHO and Lipids 6. Essential for the formation of egg, milk protein, wool and hairs of the animals. 7. Provide the basic cellular matrix within which the bone mineral matter is deposited. 8. Under condition of non-digestion and no- chances for denaturation, the protein accumulates inside the cells and produce toxicity. i.e. venoms of snakes and insects are infected by biting into the blood. 9. Endorphins (peptide) are found in brain and are involved in the suppression of pain. 62
  • 63. SIGNS OF PROTEIN DEFICIENCY 1. Anorexia 2. Reduced growth rate 3. Reduced or negative N balance 4. Reduced efficiency of feed utilisation 5. Reduced serum protein concentration 6. Anaemia 7. Fat accumulation in the liver 8. Oedema (in severe case) 9. Reduced birth weight of young 10. Reduced milk production 11. Reduced egg production in poultry 12. Reduced synthesis of certain enzymes and hormones 13. In severe protein or amino acid deficiency, growth is arrested completely 63
  • 64. Signs of Excessive Intake of Protein 1. Above certain protein intake, there is a linear depression in weight gain with increasing protein. 2. Feed intake decreases 3. Hair becomes dull and coarse 4. High protein diet reduces activity of several adipose tissue enzymes associated with fatty acid synthesis in pigs. 5. Ammonia toxicity is a practical problem in ruminants fed urea as a NPN source. Toxic symptoms in ruminants include: uneasiness, laboured breathing, excessive salivation, muscle and skin tremors, incoordination, tetany and death within 2h of onset of symptoms. 64
  • 65. MINERALS  Composed of single solid substance of uniform composition that cannot be physically separated into simpler chemical compounds  About 40 mineral elements occur in measurable amount in nature in plants and animals tissues  At least 21 of these have been shown to be required by animals  Minerals are generally classified into two categories 1. Macro (Major) Elements : Minerals required in relatively large amount by the body for normal growth and development  Mostly used in the synthesis of structural tissues 2. Micro (Minor or Trace) Elements: Minerals required in relatively small amount for the physiological processes of the body  Usually function as activators or as a component of enzyme system 65
  • 66. MINERALS MACRO  Calcium  Phosphorous  Sodium  Chlorine  Sulphur  Potassium  Magnesium MICRO  Iron & Copper  Iodine & Fluorine  Cobalt  Manganese  Molybdenum  Selenium  Zinc 66
  • 67. GENERAL FUNCTION OF MINERALS  As a constituent of skeletal structure  In regulating acid-base equilibrium  They are helpful in maintaining the colloidal state of body matter and regulating some of the physical properties of colloidal systems like viscosity, diffusion and osmotic pressure  They act as a component or an activator of enzymes and or other biological systems 67
  • 68. FUNCTIONS OF SOME MINERALS 68
  • 69. VITAMINS  Vitamins are the substances distinct from major components of food required in minute quantities and whose absence causes specific deficiency disease  Organic substances required by animals in very small amounts for regulating various body processes toward normal health, growth, production and reproduction  Cannot be synthesised by the animal and therefore must be obtained from the diet  Exception: vitamin D may be synthesised on the surface of the skin by ultra-violet irradiation, and nicotinic acid is synthesised to some extent from tryptophan 69
  • 70. CLASSIFICATION OF VITAMINS  Vitamins are grouped into 2 based upon their solubilities in either fats and fat solvents or in water 1. Fat Soluble vitamins: Vitamins that dissolve in fat  Found in in feedstuffs in association with the lipids.  These vitamins are absorbed along with the dietary fats by mechanisms similar to those involved in fat absorption  They are stored in appreciable quantities in the animal body and are not excreted in the urine 2. Water-Soluble: Vitamins that readily dissolves in water  Except for B12, are not stored but excesses are rapidly excreted in the urine  A constant dietary supply of the water- soluble vitamins is needed to avoid deficiencies 70
  • 71. FAT-SOLUBLE VITAMINS  Fat-soluble vitamins include Vit A, D, E, K 1. A - maintenance of epithelial tissue 2. D - calcium & phosphorous metabolism 3. E - antioxidant – (selenium) 4. K - blood clotting 71
  • 72. WATER SOLUBLE VITAMINS  B1- Thiamine  B2- Riboflavin  B6- Pyridoxine  Pantothenic Acid  Folic Acid  Niacin  B12- Cyanocobalamin  Choline  Biotin  C - Ascorbic Acid (not in livestock) 72
  • 73. IMPORTANT VITAMINS IN ANIMAL NUTRITION 73
  • 74. GENERAL FUNCTIONS OF VITAMINS 1. Vitamins are essential for the good health and play important role in the body growth 2. Provide resistance against diseases and increases the productivity power of animals. 3. Essential constituents of certain enzyme systems, regulate body metabolism and clotting of blood. 4. Required during pregnancy for the development of foetus. 5. Vitamin ‘A' is responsible for the proper functioning of vision and Vitamin C keeps the gums in healthy state 74
  • 75. FUNCTIONS OF SOME VITAMINS 75