A phylogenetic study og Mdm2 using tools like ClustalW2 and unrooted trees.

1. Phylogenetic Study of mdm2
Yosok PunY

2. p53 and mdm2 interactions

3. Mdm2

Negative regulator of p53 tumor suppressor
gene

Discovery from transformed mouse cell line

Mdm2 over expression with Ras oncogene led
to tumor formation in nude mice

Human homolog is sometimes called Hdm2

4. Mdm2

Increased levels of
mdm2 found in:

Soft tissue sarcomas

Osteosarcomas

Breast tumors

8. Cn3D

9. E3 ligase activity

Targets both itself and p53
for degradation by
proteasome

Inhibitor of MDM2-p53
interactions is nutlin

MDM2 also interacts with
ubiquitin protease, USP7
that reverses
ubiquitylation to prevent
degradation with
proteasome

Fine regulatory circuit

10. JalView

12. Mdm2 [Homo sapiens] FASTA
>gi|155183770|gb|ABT17086.1| Mdm2 [Homo sapiens]
MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEK
QQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQ
ELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERS
SSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSE

14. ClustalW2

17. HomoloGene
Homologs were
discovered in these
vertebrates:
Chimpanzee, Rhesus
macaque, Dog,
Cattle, House mouse,
Brown rat, Red
Junglefowl, Zebrafish

18. References

Harris. Curtis C. et. al. Proceedings of the National Academy of Sciences.
http://www.pnas.org/content/103/6/1659/F1.expansion.html. Accessed May
7th
2013.

Proteasome. Wikipedia. http://en.wikipedia.org/wiki/Proteasome. Accessed
May 7th
2013

Mdm2. Wikipedia. http://en.wikipedia.org/wiki/Mdm2. Accessed May 7th
2013.

Knappskog et. al. MDM2 promoter SNP285 and SNP309; phylogeny and
impact on cancer risk. PubMed Central.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260817/. Accessed May 13th
2013

Jayaraman A et. al. The interaction of p53 and MDM2 genes in cancers, in
silico studies and phylogenetic analysis. Biology and Medicine Vol 3 (3): 01-
12, 2011.

Databases/Tools Used: NCBI Protein. NCBI BLASTp. ClustalW2.
ClustalW2 Phylogeny. Cn3D. HomoloGene.